Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ferredoxin CarAc

Gene

carAc

Organism
Pseudomonas resinovorans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the multicomponent carbazole 1,9a-dioxygenase (CARDO), that converts carbazole (CAR) into 2-aminobiphenyl-2,3-diol. Acts as a mediator in the electron transfer from CarAd to CarAa.2 Publications

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotation2 PublicationsNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation2 Publications

pH dependencei

Optimum pH is between 7 and 7.5.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation2 Publications
Metal bindingi48 – 481Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation2 Publications
Metal bindingi65 – 651Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation2 Publications
Metal bindingi68 – 681Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation2 Publications

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB
  • dioxygenase activity Source: UniProtKB-KW
  • ferredoxin hydrogenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • carbazole catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BRENDAi1.14.12.22. 7692.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin CarAc
Alternative name(s):
Carbazole 1,9a-dioxygenase, ferredoxin component
Short name:
CARDO
Gene namesi
Name:carAc
Encoded oniPlasmid pCAR19 Publications
OrganismiPseudomonas resinovorans
Taxonomic identifieri53412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 107107Ferredoxin CarAcPRO_0000419028Add
BLAST

Interactioni

Subunit structurei

Monomer. Carbazole 1,9a-dioxygenase complex consists of a terminal oxygenase component CarAa, a ferredoxin reductase component CarAd and a ferredoxin component CarAc.3 Publications

Protein-protein interaction databases

DIPiDIP-29166N.

Structurei

Secondary structure

1
107
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Helixi11 – 133Combined sources
Beta strandi19 – 246Combined sources
Beta strandi27 – 359Combined sources
Beta strandi38 – 458Combined sources
Beta strandi47 – 504Combined sources
Helixi53 – 553Combined sources
Beta strandi56 – 594Combined sources
Beta strandi62 – 643Combined sources
Turni66 – 683Combined sources
Beta strandi71 – 733Combined sources
Turni74 – 763Combined sources
Beta strandi79 – 813Combined sources
Beta strandi93 – 10210Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCKX-ray1.90A1-107[»]
2DE5X-ray1.90D/E/F1-107[»]
2DE6X-ray1.80D/E/F1-107[»]
2DE7X-ray2.00D/E/F1-107[»]
3VMGX-ray1.95D/E/F1-107[»]
3VMHX-ray1.85D/E/F1-107[»]
3VMIX-ray2.00D/E/F1-107[»]
4NB8X-ray2.01D/E/F1-107[»]
4NB9X-ray2.05D/E/F1-107[»]
4NBAX-ray2.10D/E/F1-107[»]
4NBBX-ray2.05D/E/F1-107[»]
4NBCX-ray1.94D/E/F1-107[»]
4NBDX-ray1.95D/E1-107[»]
4NBEX-ray2.10D/E1-107[»]
4NBFX-ray2.00D/E/F1-107[»]
4NBGX-ray1.85D/E/F1-107[»]
4NBHX-ray2.15D/E/F1-107[»]
ProteinModelPortaliQ8GI16.
SMRiQ8GI16. Positions 3-107.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GI16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 10297RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK15752.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GI16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQIWLKVCA ASDMQPGTIR RVNRVGAAPL AVYRVGDQFY ATEDTCTHGI
60 70 80 90 100
ASLSEGTLDG DVIECPFHGG AFNVCTGMPA SSPCTVPLGV FEVEVKEGEV

YVAGEKK
Length:107
Mass (Da):11,367
Last modified:March 1, 2003 - v1
Checksum:i4B60F233E57C19A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047548 Genomic DNA. Translation: BAB32770.1.
AB088420 Genomic DNA. Translation: BAC41549.1.
RefSeqiNP_758571.1. NC_004444.1.
WP_011077882.1. NC_004444.1.

Genome annotation databases

GeneIDi1047047.
KEGGipg:1047047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047548 Genomic DNA. Translation: BAB32770.1.
AB088420 Genomic DNA. Translation: BAC41549.1.
RefSeqiNP_758571.1. NC_004444.1.
WP_011077882.1. NC_004444.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCKX-ray1.90A1-107[»]
2DE5X-ray1.90D/E/F1-107[»]
2DE6X-ray1.80D/E/F1-107[»]
2DE7X-ray2.00D/E/F1-107[»]
3VMGX-ray1.95D/E/F1-107[»]
3VMHX-ray1.85D/E/F1-107[»]
3VMIX-ray2.00D/E/F1-107[»]
4NB8X-ray2.01D/E/F1-107[»]
4NB9X-ray2.05D/E/F1-107[»]
4NBAX-ray2.10D/E/F1-107[»]
4NBBX-ray2.05D/E/F1-107[»]
4NBCX-ray1.94D/E/F1-107[»]
4NBDX-ray1.95D/E1-107[»]
4NBEX-ray2.10D/E1-107[»]
4NBFX-ray2.00D/E/F1-107[»]
4NBGX-ray1.85D/E/F1-107[»]
4NBHX-ray2.15D/E/F1-107[»]
ProteinModelPortaliQ8GI16.
SMRiQ8GI16. Positions 3-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29166N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1047047.
KEGGipg:1047047.

Phylogenomic databases

KOiK15752.

Enzyme and pathway databases

BRENDAi1.14.12.22. 7692.

Miscellaneous databases

EvolutionaryTraceiQ8GI16.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of genes involved in carbazole degradation of Pseudomonas sp. strain CA10: nucleotide sequences of genes and characterization of meta-cleavage enzymes and hydrolase."
    Sato S.I., Ouchiyama N., Kimura T., Nojiri H., Yamane H., Omori T.
    J. Bacteriol. 179:4841-4849(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NOMENCLATURE.
    Strain: CA10.
  2. "Identification and characterization of genes encoding carbazole 1,9a-dioxygenase in Pseudomonas sp. strain CA10."
    Sato S.I., Nam J.W., Kasuga K., Nojiri H., Yamane H., Omori T.
    J. Bacteriol. 179:4850-4858(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN THE CARBAZOLE DEGRADATION.
    Strain: CA10.
  3. "Genetic characterization and evolutionary implications of a car gene cluster in the carbazole degrader Pseudomonas sp. strain CA10."
    Nojiri H., Sekiguchi H., Maeda K., Urata M., Nakai S., Yoshida T., Habe H., Omori T.
    J. Bacteriol. 183:3663-3679(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CA10.
  4. "Complete nucleotide sequence of carbazole/dioxin-degrading plasmid pCAR1 in Pseudomonas resinovorans strain CA10 indicates its mosaicity and the presence of large catabolic transposon Tn4676."
    Maeda K., Nojiri H., Shintani M., Yoshida T., Habe H., Omori T.
    J. Mol. Biol. 326:21-33(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  5. "Transcriptional regulation of the ant operon, encoding two-component anthranilate 1,2-dioxygenase, on the carbazole-degradative plasmid pCAR1 of Pseudomonas resinovorans strain CA10."
    Urata M., Miyakoshi M., Kai S., Maeda K., Habe H., Omori T., Yamane H., Nojiri H.
    J. Bacteriol. 186:6815-6823(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CA10.
    Plasmid: pCAR1
  6. "Characterization of the replication, maintenance, and transfer features of the IncP-7 plasmid pCAR1, which carries genes involved in carbazole and dioxin degradation."
    Shintani M., Yano H., Habe H., Omori T., Yamane H., Tsuda M., Nojiri H.
    Appl. Environ. Microbiol. 72:3206-3216(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  7. "Transcriptome analysis of Pseudomonas putida KT2440 harboring the completely sequenced IncP-7 plasmid pCAR1."
    Miyakoshi M., Shintani M., Terabayashi T., Kai S., Yamane H., Nojiri H.
    J. Bacteriol. 189:6849-6860(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  8. "Carbazole-degradative IncP-7 plasmid pCAR1.2 is structurally unstable in Pseudomonas fluorescens Pf0-1, which accumulates catechol, the intermediate of the carbazole degradation pathway."
    Takahashi Y., Shintani M., Li L., Yamane H., Nojiri H.
    Appl. Environ. Microbiol. 75:3920-3929(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  9. "The complete nucleotide sequence of pCAR2: pCAR2 and pCAR1 were structurally identical IncP-7 carbazole degradative plasmids."
    Takahashi Y., Shintani M., Yamane H., Nojiri H.
    Biosci. Biotechnol. Biochem. 73:744-746(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  10. "High-resolution mapping of plasmid transcriptomes in different host bacteria."
    Miyakoshi M., Nishida H., Shintani M., Yamane H., Nojiri H.
    BMC Genomics 10:12-12(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  11. "Response of the Pseudomonas host chromosomal transcriptome to carriage of the IncP-7 plasmid pCAR1."
    Shintani M., Takahashi Y., Tokumaru H., Kadota K., Hara H., Miyakoshi M., Naito K., Yamane H., Nishida H., Nojiri H.
    Environ. Microbiol. 12:1413-1426(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  12. "Pmr, a histone-like protein H1 (H-NS) family protein encoded by the IncP-7 plasmid pCAR1, is a key global regulator that alters host function."
    Yun C.S., Suzuki C., Naito K., Takeda T., Takahashi Y., Sai F., Terabayashi T., Miyakoshi M., Shintani M., Nishida H., Yamane H., Nojiri H.
    J. Bacteriol. 192:4720-4731(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pCAR1
  13. "Purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10."
    Nam J.W., Nojiri H., Noguchi H., Uchimura H., Yoshida T., Habe H., Yamane H., Omori T.
    Appl. Environ. Microbiol. 68:5882-5890(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION AS A FERREDOXIN, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT.
    Strain: CA10.
  14. "Crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-heme iron oxygenase system."
    Nam J.W., Noguchi H., Fujimoto Z., Mizuno H., Ashikawa Y., Abo M., Fushinobu S., Kobashi N., Wakagi T., Iwata K., Yoshida T., Habe H., Yamane H., Omori T., Nojiri H.
    Proteins 58:779-789(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (2FE-2S).
    Strain: CA10.
  15. "Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system."
    Ashikawa Y., Fujimoto Z., Noguchi H., Habe H., Omori T., Yamane H., Nojiri H.
    Structure 14:1779-1789(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (2FE-2S), COFACTOR.

Entry informationi

Entry nameiCARAC_PSERE
AccessioniPrimary (citable) accession number: Q8GI16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2003
Last modified: October 14, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.