Q8GI16 (CARAC_PSERE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferredoxin CarAc

Alternative name(s):
Carbazole 1,9a-dioxygenase, ferredoxin component
Short name=CARDO

Gene names

Name:

carAc

Encoded on

Plasmid pCAR1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Organism

Pseudomonas resinovorans

Taxonomic identifier

53412 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the multicomponent carbazole 1,9a-dioxygenase (CARDO), that converts carbazole (CAR) into 2-aminobiphenyl-2,3-diol. Acts as a mediator in the electron transfer from CarAd to CarAa. Ref.2 Ref.13
Cofactor	Binds 1 2Fe-2S cluster per subunit. Ref.13 Ref.15
Subunit structure	Monomer. Carbazole 1,9a-dioxygenase complex consists of a terminal oxygenase component CarAa, a ferredoxin reductase component CarAd and a ferredoxin component CarAc. Ref.13
Sequence similarities	Contains 1 Rieske domain.
Biophysicochemical properties	pH dependence: Optimum pH is between 7 and 7.5. Ref.13 Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	carbazole catabolic process Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from direct assay Ref.15. Source: UniProtKB ferredoxin hydrogenase activity Inferred from direct assay Ref.13. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 107

107

Ferredoxin CarAc

PRO_0000419028

Regions

Domain

6 – 102

97

Rieske

Sites

Metal binding

46

1

Iron-sulfur (2Fe-2S)

Metal binding

48

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Metal binding

65

1

Iron-sulfur (2Fe-2S)

Metal binding

68

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

107

Helix Strand Turn

Details...

Beta strand

6 – 10

5

Helix

11 – 13

3

Beta strand

19 – 24

6

Beta strand

27 – 35

9

Beta strand

38 – 45

8

Beta strand

47 – 50

4

Helix

53 – 55

3

Beta strand

56 – 59

4

Beta strand

62 – 64

3

Turn

66 – 68

3

Beta strand

71 – 73

3

Turn

74 – 76

3

Beta strand

79 – 81

3

Beta strand

93 – 102

10

Sequences

	Sequence		Length	Mass (Da)
	Q8GI16 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4B60F233E57C19A4	FASTA	107	11,367
	10 20 30 40 50 60 MNQIWLKVCA ASDMQPGTIR RVNRVGAAPL AVYRVGDQFY ATEDTCTHGI ASLSEGTLDG 70 80 90 100 DVIECPFHGG AFNVCTGMPA SSPCTVPLGV FEVEVKEGEV YVAGEKK

References

[1]	"Cloning of genes involved in carbazole degradation of Pseudomonas sp. strain CA10: nucleotide sequences of genes and characterization of meta-cleavage enzymes and hydrolase." Sato S.I., Ouchiyama N., Kimura T., Nojiri H., Yamane H., Omori T. J. Bacteriol. 179:4841-4849(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NOMENCLATURE. Strain: CA10.
[2]	"Identification and characterization of genes encoding carbazole 1,9a-dioxygenase in Pseudomonas sp. strain CA10." Sato S.I., Nam J.W., Kasuga K., Nojiri H., Yamane H., Omori T. J. Bacteriol. 179:4850-4858(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN THE CARBAZOLE DEGRADATION. Strain: CA10.
[3]	"Genetic characterization and evolutionary implications of a car gene cluster in the carbazole degrader Pseudomonas sp. strain CA10." Nojiri H., Sekiguchi H., Maeda K., Urata M., Nakai S., Yoshida T., Habe H., Omori T. J. Bacteriol. 183:3663-3679(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CA10.
[4]	"Complete nucleotide sequence of carbazole/dioxin-degrading plasmid pCAR1 in Pseudomonas resinovorans strain CA10 indicates its mosaicity and the presence of large catabolic transposon Tn4676." Maeda K., Nojiri H., Shintani M., Yoshida T., Habe H., Omori T. J. Mol. Biol. 326:21-33(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Transcriptional regulation of the ant operon, encoding two-component anthranilate 1,2-dioxygenase, on the carbazole-degradative plasmid pCAR1 of Pseudomonas resinovorans strain CA10." Urata M., Miyakoshi M., Kai S., Maeda K., Habe H., Omori T., Yamane H., Nojiri H. J. Bacteriol. 186:6815-6823(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CA10.
[6]	"Characterization of the replication, maintenance, and transfer features of the IncP-7 plasmid pCAR1, which carries genes involved in carbazole and dioxin degradation." Shintani M., Yano H., Habe H., Omori T., Yamane H., Tsuda M., Nojiri H. Appl. Environ. Microbiol. 72:3206-3216(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"Transcriptome analysis of Pseudomonas putida KT2440 harboring the completely sequenced IncP-7 plasmid pCAR1." Miyakoshi M., Shintani M., Terabayashi T., Kai S., Yamane H., Nojiri H. J. Bacteriol. 189:6849-6860(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	"Carbazole-degradative IncP-7 plasmid pCAR1.2 is structurally unstable in Pseudomonas fluorescens Pf0-1, which accumulates catechol, the intermediate of the carbazole degradation pathway." Takahashi Y., Shintani M., Li L., Yamane H., Nojiri H. Appl. Environ. Microbiol. 75:3920-3929(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[9]	"The complete nucleotide sequence of pCAR2: pCAR2 and pCAR1 were structurally identical IncP-7 carbazole degradative plasmids." Takahashi Y., Shintani M., Yamane H., Nojiri H. Biosci. Biotechnol. Biochem. 73:744-746(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[10]	"High-resolution mapping of plasmid transcriptomes in different host bacteria." Miyakoshi M., Nishida H., Shintani M., Yamane H., Nojiri H. BMC Genomics 10:12-12(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[11]	"Response of the Pseudomonas host chromosomal transcriptome to carriage of the IncP-7 plasmid pCAR1." Shintani M., Takahashi Y., Tokumaru H., Kadota K., Hara H., Miyakoshi M., Naito K., Yamane H., Nishida H., Nojiri H. Environ. Microbiol. 12:1413-1426(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[12]	"Pmr, a histone-like protein H1 (H-NS) family protein encoded by the IncP-7 plasmid pCAR1, is a key global regulator that alters host function." Yun C.S., Suzuki C., Naito K., Takeda T., Takahashi Y., Sai F., Terabayashi T., Miyakoshi M., Shintani M., Nishida H., Yamane H., Nojiri H. J. Bacteriol. 192:4720-4731(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[13]	"Purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10." Nam J.W., Nojiri H., Noguchi H., Uchimura H., Yoshida T., Habe H., Yamane H., Omori T. Appl. Environ. Microbiol. 68:5882-5890(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION AS A FERREDOXIN, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT. Strain: CA10.
[14]	"Crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-heme iron oxygenase system." Nam J.W., Noguchi H., Fujimoto Z., Mizuno H., Ashikawa Y., Abo M., Fushinobu S., Kobashi N., Wakagi T., Iwata K., Yoshida T., Habe H., Yamane H., Omori T., Nojiri H. Proteins 58:779-789(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (2FE-2S). Strain: CA10.
[15]	"Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system." Ashikawa Y., Fujimoto Z., Noguchi H., Habe H., Omori T., Yamane H., Nojiri H. Structure 14:1779-1789(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (2FE-2S), COFACTOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB047548 Genomic DNA. Translation: BAB32770.1.
AB088420 Genomic DNA. Translation: BAC41549.1.

RefSeq

NP_758571.1. NC_004444.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VCK	X-ray	1.90	A	1-107	[»]
2DE5	X-ray	1.90	D/E/F	1-107	[»]
2DE6	X-ray	1.80	D/E/F	1-107	[»]
2DE7	X-ray	2.00	D/E/F	1-107	[»]
3VMG	X-ray	1.95	D/E/F	1-107	[»]
3VMH	X-ray	1.85	D/E/F	1-107	[»]
3VMI	X-ray	2.00	D/E/F	1-107	[»]

ProteinModelPortal

Q8GI16.

SMR

Q8GI16. Positions 3-107.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29166N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1047047.

Phylogenomic databases

ProtClustDB

CLSK2462194.

Family and domain databases

Gene3D

2.102.10.10. 1 hit.

InterPro

IPR017941. Rieske_2Fe-2S.
[Graphical view]

Pfam

PF00355. Rieske. 1 hit.
[Graphical view]

SUPFAM

SSF50022. Rieske_dom. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8GI16.

Entry information

Entry name

CARAC_PSERE

Accession

Primary (citable) accession number: Q8GI16

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program