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Protein

Ferredoxin CarAc

Gene

carAc

Organism
Pseudomonas resinovorans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the multicomponent carbazole 1,9a-dioxygenase (CARDO), that converts carbazole (CAR) into 2-aminobiphenyl-2,3-diol. Acts as a mediator in the electron transfer from CarAd to CarAa.2 Publications

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotation2 PublicationsNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation2 Publications

pH dependencei

Optimum pH is between 7 and 7.5.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation2 Publications
Metal bindingi48 – 481Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation2 Publications
Metal bindingi65 – 651Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation2 Publications
Metal bindingi68 – 681Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation2 Publications

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB
  • dioxygenase activity Source: UniProtKB-KW
  • ferredoxin hydrogenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • carbazole catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BRENDAi1.14.12.22. 7692.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin CarAc
Alternative name(s):
Carbazole 1,9a-dioxygenase, ferredoxin component
Short name:
CARDO
Gene namesi
Name:carAc
Encoded oniPlasmid pCAR19 Publications
OrganismiPseudomonas resinovorans
Taxonomic identifieri53412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 107107Ferredoxin CarAcPRO_0000419028Add
BLAST

Interactioni

Subunit structurei

Monomer. Carbazole 1,9a-dioxygenase complex consists of a terminal oxygenase component CarAa, a ferredoxin reductase component CarAd and a ferredoxin component CarAc.3 Publications

Protein-protein interaction databases

DIPiDIP-29166N.

Structurei

Secondary structure

1
107
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Helixi11 – 133Combined sources
Beta strandi19 – 246Combined sources
Beta strandi27 – 359Combined sources
Beta strandi38 – 458Combined sources
Beta strandi47 – 504Combined sources
Helixi53 – 553Combined sources
Beta strandi56 – 594Combined sources
Beta strandi62 – 643Combined sources
Turni66 – 683Combined sources
Beta strandi71 – 733Combined sources
Turni74 – 763Combined sources
Beta strandi79 – 813Combined sources
Beta strandi93 – 10210Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCKX-ray1.90A1-107[»]
2DE5X-ray1.90D/E/F1-107[»]
2DE6X-ray1.80D/E/F1-107[»]
2DE7X-ray2.00D/E/F1-107[»]
3VMGX-ray1.95D/E/F1-107[»]
3VMHX-ray1.85D/E/F1-107[»]
3VMIX-ray2.00D/E/F1-107[»]
4NB8X-ray2.01D/E/F1-107[»]
4NB9X-ray2.05D/E/F1-107[»]
4NBAX-ray2.10D/E/F1-107[»]
4NBBX-ray2.05D/E/F1-107[»]
4NBCX-ray1.94D/E/F1-107[»]
4NBDX-ray1.95D/E1-107[»]
4NBEX-ray2.10D/E1-107[»]
4NBFX-ray2.00D/E/F1-107[»]
4NBGX-ray1.85D/E/F1-107[»]
4NBHX-ray2.15D/E/F1-107[»]
ProteinModelPortaliQ8GI16.
SMRiQ8GI16. Positions 3-107.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GI16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 10297RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK15752.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GI16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQIWLKVCA ASDMQPGTIR RVNRVGAAPL AVYRVGDQFY ATEDTCTHGI
60 70 80 90 100
ASLSEGTLDG DVIECPFHGG AFNVCTGMPA SSPCTVPLGV FEVEVKEGEV

YVAGEKK
Length:107
Mass (Da):11,367
Last modified:March 1, 2003 - v1
Checksum:i4B60F233E57C19A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047548 Genomic DNA. Translation: BAB32770.1.
AB088420 Genomic DNA. Translation: BAC41549.1.
RefSeqiNP_758571.1. NC_004444.1.
WP_011077882.1. NC_004444.1.

Genome annotation databases

GeneIDi1047047.
KEGGipg:1047047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047548 Genomic DNA. Translation: BAB32770.1.
AB088420 Genomic DNA. Translation: BAC41549.1.
RefSeqiNP_758571.1. NC_004444.1.
WP_011077882.1. NC_004444.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCKX-ray1.90A1-107[»]
2DE5X-ray1.90D/E/F1-107[»]
2DE6X-ray1.80D/E/F1-107[»]
2DE7X-ray2.00D/E/F1-107[»]
3VMGX-ray1.95D/E/F1-107[»]
3VMHX-ray1.85D/E/F1-107[»]
3VMIX-ray2.00D/E/F1-107[»]
4NB8X-ray2.01D/E/F1-107[»]
4NB9X-ray2.05D/E/F1-107[»]
4NBAX-ray2.10D/E/F1-107[»]
4NBBX-ray2.05D/E/F1-107[»]
4NBCX-ray1.94D/E/F1-107[»]
4NBDX-ray1.95D/E1-107[»]
4NBEX-ray2.10D/E1-107[»]
4NBFX-ray2.00D/E/F1-107[»]
4NBGX-ray1.85D/E/F1-107[»]
4NBHX-ray2.15D/E/F1-107[»]
ProteinModelPortaliQ8GI16.
SMRiQ8GI16. Positions 3-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29166N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1047047.
KEGGipg:1047047.

Phylogenomic databases

KOiK15752.

Enzyme and pathway databases

BRENDAi1.14.12.22. 7692.

Miscellaneous databases

EvolutionaryTraceiQ8GI16.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARAC_PSERE
AccessioniPrimary (citable) accession number: Q8GI16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2003
Last modified: October 14, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.