Q8GGG1 (WSD_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
O-acyltransferase WSD

Alternative name(s):
Diacylglycerol O-acyltransferase
Short name=DGAT
EC=2.3.1.20
Long-chain-alcohol O-fatty-acyltransferase
EC=2.3.1.75
Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
Wax synthase
Short name=WS

Gene names

Name:	wax-dgaT
Ordered Locus Names:	ACIAD0832

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS uses C(12)-CoA to C(18)-CoA substrates whereas DGAT prefers C(20)-CoA. Upon expression in E.coli and Pseudomonas citronellolis (DSM 50332) both WS and DGAT activities increase.
Catalytic activity	Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester. Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Disruption phenotype	Cells lacking this gene do not make wax ester and only trace amounts of triacylglycerol. Ref.1
Sequence similarities	Belongs to the long-chain O-acyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=15.6 µM for palmitoyl-CoA (C16, in wax synthase) Ref.1 KM=21.1 µM for palmitoyl-CoA (diacylglycerol acyltransferase) Vmax=212.8 pmol/min/mg enzyme (wax synthase) Vmax=54.3 pmol/min/mg enzyme (diacylglycerol acyltransferase)

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid biosynthesis Lipid metabolism
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC long-chain-alcohol O-fatty-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	O-acyltransferase WSD		PRO_0000393350
Sites
	Active site	133	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8GGG1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: AEFB9A1C83FE3823	FASTA	458	51,780
	10 20 30 40 50 60 MRPLHPIDFI FLSLEKRQQP MHVGGLFLFQ IPDNAPDTFI QDLVNDIRIS KSIPVPPFNN 70 80 90 100 110 120 KLNGLFWDED EEFDLDHHFR HIALPHPGRI RELLIYISQE HSTLLDRAKP LWTCNIIEGI 130 140 150 160 170 180 EGNRFAMYFK IHHAMVDGVA GMRLIEKSLS HDVTEKSIVP PWCVEGKRAK RLREPKTGKI 190 200 210 220 230 240 KKIMSGIKSQ LQATPTVIQE LSQTVFKDIG RNPDHVSSFQ APCSILNQRV SSSRRFAAQS 250 260 270 280 290 300 FDLDRFRNIA KSLNVTINDV VLAVCSGALR AYLMSHNSLP SKPLIAMVPA SIRNDDSDVS 310 320 330 340 350 360 NRITMILANL ATHKDDPLQR LEIIRRSVQN SKQRFKRMTS DQILNYSAVV YGPAGLNIIS 370 380 390 400 410 420 GMMPKRQAFN LVISNVPGPR EPLYWNGAKL DALYPASIVL DGQALNITMT SYLDKLEVGL 430 440 450 IACRNALPRM QNLLTHLEEE IQLFEGVIAK QEDIKTAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1." Kalscheuer R., Steinbuchel A. J. Biol. Chem. 278:8075-8082(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES, EXPRESSION IN OTHER BACTERIA, DISRUPTION PHENOTYPE.
[2]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF529086 Genomic DNA. Translation: AAO17391.1. CR543861 Genomic DNA. Translation: CAG67733.1.
RefSeq	YP_045555.1. NC_005966.1.
3D structure databases
ProteinModelPortal	Q8GGG1.
ModBase	Search...
Protein-protein interaction databases
STRING	62977.ACIAD0832.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG67733; CAG67733; ACIAD0832.
GeneID	2879218.
KEGG	aci:ACIAD0832.
PATRIC	20739558. VBIAciSp98416_0743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG09285.
HOGENOM	HOG000277728.
KO	K00635.
OMA	APRTMLN.
ProtClustDB	CLSK830521.
Enzyme and pathway databases
BioCyc	ASP62977:GJVV-784-MONOMER. MetaCyc:ACIAD0832-MONOMER.
BRENDA	2.3.1.20. 99.
UniPathway	UPA00282.
Family and domain databases
InterPro	IPR014292. Acyl_transf_WS/DGAT. IPR009721. O-acyltransferase_WSD1_C. IPR004255. O-acyltransferase_WSD1_N. [Graphical view]
Pfam	PF06974. DUF1298. 1 hit. PF03007. WES_acyltransf. 1 hit. [Graphical view]
TIGRFAMs	TIGR02946. acyl_WS_DGAT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

WSD_ACIAD

Accession

Primary (citable) accession number: Q8GGG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program