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Q8GGG1 (WSD_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
O-acyltransferase WSD
Alternative name(s):
Diacylglycerol O-acyltransferase
Short name=DGAT
EC=2.3.1.20
Long-chain-alcohol O-fatty-acyltransferase
EC=2.3.1.75
Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
Wax synthase
Short name=WS
Gene names
Name:wax-dgaT
Ordered Locus Names:ACIAD0832
OrganismAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) [Complete proteome] [HAMAP]
Taxonomic identifier62977 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS uses C(12)-CoA to C(18)-CoA substrates whereas DGAT prefers C(20)-CoA. Upon expression in E.coli and Pseudomonas citronellolis (DSM 50332) both WS and DGAT activities increase.

Catalytic activity

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Pathway

Glycerolipid metabolism; triacylglycerol biosynthesis.

Disruption phenotype

Cells lacking this gene do not make wax ester and only trace amounts of triacylglycerol. Ref.1

Sequence similarities

Belongs to the long-chain O-acyltransferase family.

Biophysicochemical properties

Kinetic parameters:

KM=15.6 µM for palmitoyl-CoA (C16, in wax synthase) Ref.1

KM=21.1 µM for palmitoyl-CoA (diacylglycerol acyltransferase)

Vmax=212.8 pmol/min/mg enzyme (wax synthase)

Vmax=54.3 pmol/min/mg enzyme (diacylglycerol acyltransferase)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 458458O-acyltransferase WSD
PRO_0000393350

Sites

Active site1331Proton acceptor Potential

Sequences

Sequence LengthMass (Da)Tools
Q8GGG1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: AEFB9A1C83FE3823

FASTA45851,780
        10         20         30         40         50         60 
MRPLHPIDFI FLSLEKRQQP MHVGGLFLFQ IPDNAPDTFI QDLVNDIRIS KSIPVPPFNN 

        70         80         90        100        110        120 
KLNGLFWDED EEFDLDHHFR HIALPHPGRI RELLIYISQE HSTLLDRAKP LWTCNIIEGI 

       130        140        150        160        170        180 
EGNRFAMYFK IHHAMVDGVA GMRLIEKSLS HDVTEKSIVP PWCVEGKRAK RLREPKTGKI 

       190        200        210        220        230        240 
KKIMSGIKSQ LQATPTVIQE LSQTVFKDIG RNPDHVSSFQ APCSILNQRV SSSRRFAAQS 

       250        260        270        280        290        300 
FDLDRFRNIA KSLNVTINDV VLAVCSGALR AYLMSHNSLP SKPLIAMVPA SIRNDDSDVS 

       310        320        330        340        350        360 
NRITMILANL ATHKDDPLQR LEIIRRSVQN SKQRFKRMTS DQILNYSAVV YGPAGLNIIS 

       370        380        390        400        410        420 
GMMPKRQAFN LVISNVPGPR EPLYWNGAKL DALYPASIVL DGQALNITMT SYLDKLEVGL 

       430        440        450 
IACRNALPRM QNLLTHLEEE IQLFEGVIAK QEDIKTAN 

« Hide

References

« Hide 'large scale' references
[1]"A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1."
Kalscheuer R., Steinbuchel A.
J. Biol. Chem. 278:8075-8082(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES, EXPRESSION IN OTHER BACTERIA, DISRUPTION PHENOTYPE.
[2]"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33305 / BD413 / ADP1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF529086 Genomic DNA. Translation: AAO17391.1.
CR543861 Genomic DNA. Translation: CAG67733.1.
RefSeqYP_045555.1. NC_005966.1.

3D structure databases

ProteinModelPortalQ8GGG1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING62977.ACIAD0832.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG67733; CAG67733; ACIAD0832.
GeneID2879218.
KEGGaci:ACIAD0832.
PATRIC20739558. VBIAciSp98416_0743.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG09285.
HOGENOMHOG000277728.
KOK00635.
OMAAKSTEYV.
OrthoDBEOG6XWTZM.

Enzyme and pathway databases

BioCycASP62977:GJVV-784-MONOMER.
MetaCyc:ACIAD0832-MONOMER.
BRENDA2.3.1.20. 99.
UniPathwayUPA00282.

Family and domain databases

InterProIPR014292. Acyl_transf_WS/DGAT.
IPR009721. O-acyltransferase_WSD1_C.
IPR004255. O-acyltransferase_WSD1_N.
[Graphical view]
PfamPF06974. DUF1298. 1 hit.
PF03007. WES_acyltransf. 1 hit.
[Graphical view]
TIGRFAMsTIGR02946. acyl_WS_DGAT. 1 hit.
ProtoNetSearch...

Entry information

Entry nameWSD_ACIAD
AccessionPrimary (citable) accession number: Q8GGG1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways