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Q8GGG1

- WSD_ACIAD

UniProt

Q8GGG1 - WSD_ACIAD

Protein

O-acyltransferase WSD

Gene

wax-dgaT

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS uses C(12)-CoA to C(18)-CoA substrates whereas DGAT prefers C(20)-CoA. Upon expression in E.coli and Pseudomonas citronellolis (DSM 50332) both WS and DGAT activities increase.

    Catalytic activityi

    Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.
    Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

    Kineticsi

    1. KM=15.6 µM for palmitoyl-CoA (C(16), in wax synthase)1 Publication
    2. KM=21.1 µM for palmitoyl-CoA (diacylglycerol acyltransferase)1 Publication

    Vmax=212.8 pmol/min/mg enzyme (wax synthase)1 Publication

    Vmax=54.3 pmol/min/mg enzyme (diacylglycerol acyltransferase)1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei133 – 1331Proton acceptorSequence Analysis

    GO - Molecular functioni

    1. diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
    2. long-chain-alcohol O-fatty-acyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycerol metabolic process Source: UniProtKB-KW
    2. triglyceride biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciASP62977:GJVV-784-MONOMER.
    MetaCyc:ACIAD0832-MONOMER.
    BRENDAi2.3.1.20. 99.
    UniPathwayiUPA00282.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    O-acyltransferase WSD
    Alternative name(s):
    Diacylglycerol O-acyltransferase (EC:2.3.1.20)
    Short name:
    DGAT
    Long-chain-alcohol O-fatty-acyltransferase (EC:2.3.1.75)
    Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
    Wax synthase
    Short name:
    WS
    Gene namesi
    Name:wax-dgaT
    Ordered Locus Names:ACIAD0832
    OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
    Taxonomic identifieri62977 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
    ProteomesiUP000000430: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene do not make wax ester and only trace amounts of triacylglycerol.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 458458O-acyltransferase WSDPRO_0000393350Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi62977.ACIAD0832.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GGG1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG09285.
    HOGENOMiHOG000277728.
    KOiK00635.
    OMAiAKSTEYV.
    OrthoDBiEOG6XWTZM.

    Family and domain databases

    InterProiIPR014292. Acyl_transf_WS/DGAT.
    IPR009721. O-acyltransferase_WSD1_C.
    IPR004255. O-acyltransferase_WSD1_N.
    [Graphical view]
    PfamiPF06974. DUF1298. 1 hit.
    PF03007. WES_acyltransf. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02946. acyl_WS_DGAT. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8GGG1-1 [UniParc]FASTAAdd to Basket

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    MRPLHPIDFI FLSLEKRQQP MHVGGLFLFQ IPDNAPDTFI QDLVNDIRIS    50
    KSIPVPPFNN KLNGLFWDED EEFDLDHHFR HIALPHPGRI RELLIYISQE 100
    HSTLLDRAKP LWTCNIIEGI EGNRFAMYFK IHHAMVDGVA GMRLIEKSLS 150
    HDVTEKSIVP PWCVEGKRAK RLREPKTGKI KKIMSGIKSQ LQATPTVIQE 200
    LSQTVFKDIG RNPDHVSSFQ APCSILNQRV SSSRRFAAQS FDLDRFRNIA 250
    KSLNVTINDV VLAVCSGALR AYLMSHNSLP SKPLIAMVPA SIRNDDSDVS 300
    NRITMILANL ATHKDDPLQR LEIIRRSVQN SKQRFKRMTS DQILNYSAVV 350
    YGPAGLNIIS GMMPKRQAFN LVISNVPGPR EPLYWNGAKL DALYPASIVL 400
    DGQALNITMT SYLDKLEVGL IACRNALPRM QNLLTHLEEE IQLFEGVIAK 450
    QEDIKTAN 458
    Length:458
    Mass (Da):51,780
    Last modified:March 1, 2003 - v1
    Checksum:iAEFB9A1C83FE3823
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF529086 Genomic DNA. Translation: AAO17391.1.
    CR543861 Genomic DNA. Translation: CAG67733.1.
    RefSeqiYP_045555.1. NC_005966.1.

    Genome annotation databases

    EnsemblBacteriaiCAG67733; CAG67733; ACIAD0832.
    GeneIDi2879218.
    KEGGiaci:ACIAD0832.
    PATRICi20739558. VBIAciSp98416_0743.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF529086 Genomic DNA. Translation: AAO17391.1 .
    CR543861 Genomic DNA. Translation: CAG67733.1 .
    RefSeqi YP_045555.1. NC_005966.1.

    3D structure databases

    ProteinModelPortali Q8GGG1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 62977.ACIAD0832.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG67733 ; CAG67733 ; ACIAD0832 .
    GeneIDi 2879218.
    KEGGi aci:ACIAD0832.
    PATRICi 20739558. VBIAciSp98416_0743.

    Phylogenomic databases

    eggNOGi NOG09285.
    HOGENOMi HOG000277728.
    KOi K00635.
    OMAi AKSTEYV.
    OrthoDBi EOG6XWTZM.

    Enzyme and pathway databases

    UniPathwayi UPA00282 .
    BioCyci ASP62977:GJVV-784-MONOMER.
    MetaCyc:ACIAD0832-MONOMER.
    BRENDAi 2.3.1.20. 99.

    Family and domain databases

    InterProi IPR014292. Acyl_transf_WS/DGAT.
    IPR009721. O-acyltransferase_WSD1_C.
    IPR004255. O-acyltransferase_WSD1_N.
    [Graphical view ]
    Pfami PF06974. DUF1298. 1 hit.
    PF03007. WES_acyltransf. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02946. acyl_WS_DGAT. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1."
      Kalscheuer R., Steinbuchel A.
      J. Biol. Chem. 278:8075-8082(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES, EXPRESSION IN OTHER BACTERIA, DISRUPTION PHENOTYPE.
    2. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
      Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
      Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33305 / BD413 / ADP1.

    Entry informationi

    Entry nameiWSD_ACIAD
    AccessioniPrimary (citable) accession number: Q8GGG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    External Data

    Dasty 3