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Q8GGG1

- WSD_ACIAD

UniProt

Q8GGG1 - WSD_ACIAD

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Protein

O-acyltransferase WSD

Gene

wax-dgaT

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS uses C(12)-CoA to C(18)-CoA substrates whereas DGAT prefers C(20)-CoA. Upon expression in E.coli and Pseudomonas citronellolis (DSM 50332) both WS and DGAT activities increase.

Catalytic activityi

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Kineticsi

  1. KM=15.6 µM for palmitoyl-CoA (C(16), in wax synthase)1 Publication
  2. KM=21.1 µM for palmitoyl-CoA (diacylglycerol acyltransferase)1 Publication

Vmax=212.8 pmol/min/mg enzyme (wax synthase)1 Publication

Vmax=54.3 pmol/min/mg enzyme (diacylglycerol acyltransferase)1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei133 – 1331Proton acceptorSequence Analysis

GO - Molecular functioni

  1. diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
  2. long-chain-alcohol O-fatty-acyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerol metabolic process Source: UniProtKB-KW
  2. triglyceride biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciASP62977:GJVV-784-MONOMER.
MetaCyc:ACIAD0832-MONOMER.
BRENDAi2.3.1.20. 99.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
O-acyltransferase WSD
Alternative name(s):
Diacylglycerol O-acyltransferase (EC:2.3.1.20)
Short name:
DGAT
Long-chain-alcohol O-fatty-acyltransferase (EC:2.3.1.75)
Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
Wax synthase
Short name:
WS
Gene namesi
Name:wax-dgaT
Ordered Locus Names:ACIAD0832
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not make wax ester and only trace amounts of triacylglycerol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458O-acyltransferase WSDPRO_0000393350Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi62977.ACIAD0832.

Structurei

3D structure databases

ProteinModelPortaliQ8GGG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG09285.
HOGENOMiHOG000277728.
KOiK00635.
OMAiAKSTEYV.
OrthoDBiEOG6XWTZM.

Family and domain databases

InterProiIPR014292. Acyl_transf_WS/DGAT.
IPR009721. O-acyltransferase_WSD1_C.
IPR004255. O-acyltransferase_WSD1_N.
[Graphical view]
PfamiPF06974. DUF1298. 1 hit.
PF03007. WES_acyltransf. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02946. acyl_WS_DGAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GGG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRPLHPIDFI FLSLEKRQQP MHVGGLFLFQ IPDNAPDTFI QDLVNDIRIS
60 70 80 90 100
KSIPVPPFNN KLNGLFWDED EEFDLDHHFR HIALPHPGRI RELLIYISQE
110 120 130 140 150
HSTLLDRAKP LWTCNIIEGI EGNRFAMYFK IHHAMVDGVA GMRLIEKSLS
160 170 180 190 200
HDVTEKSIVP PWCVEGKRAK RLREPKTGKI KKIMSGIKSQ LQATPTVIQE
210 220 230 240 250
LSQTVFKDIG RNPDHVSSFQ APCSILNQRV SSSRRFAAQS FDLDRFRNIA
260 270 280 290 300
KSLNVTINDV VLAVCSGALR AYLMSHNSLP SKPLIAMVPA SIRNDDSDVS
310 320 330 340 350
NRITMILANL ATHKDDPLQR LEIIRRSVQN SKQRFKRMTS DQILNYSAVV
360 370 380 390 400
YGPAGLNIIS GMMPKRQAFN LVISNVPGPR EPLYWNGAKL DALYPASIVL
410 420 430 440 450
DGQALNITMT SYLDKLEVGL IACRNALPRM QNLLTHLEEE IQLFEGVIAK

QEDIKTAN
Length:458
Mass (Da):51,780
Last modified:March 1, 2003 - v1
Checksum:iAEFB9A1C83FE3823
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF529086 Genomic DNA. Translation: AAO17391.1.
CR543861 Genomic DNA. Translation: CAG67733.1.
RefSeqiYP_045555.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG67733; CAG67733; ACIAD0832.
GeneIDi2879218.
KEGGiaci:ACIAD0832.
PATRICi20739558. VBIAciSp98416_0743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF529086 Genomic DNA. Translation: AAO17391.1 .
CR543861 Genomic DNA. Translation: CAG67733.1 .
RefSeqi YP_045555.1. NC_005966.1.

3D structure databases

ProteinModelPortali Q8GGG1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 62977.ACIAD0832.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG67733 ; CAG67733 ; ACIAD0832 .
GeneIDi 2879218.
KEGGi aci:ACIAD0832.
PATRICi 20739558. VBIAciSp98416_0743.

Phylogenomic databases

eggNOGi NOG09285.
HOGENOMi HOG000277728.
KOi K00635.
OMAi AKSTEYV.
OrthoDBi EOG6XWTZM.

Enzyme and pathway databases

UniPathwayi UPA00282 .
BioCyci ASP62977:GJVV-784-MONOMER.
MetaCyc:ACIAD0832-MONOMER.
BRENDAi 2.3.1.20. 99.

Family and domain databases

InterProi IPR014292. Acyl_transf_WS/DGAT.
IPR009721. O-acyltransferase_WSD1_C.
IPR004255. O-acyltransferase_WSD1_N.
[Graphical view ]
Pfami PF06974. DUF1298. 1 hit.
PF03007. WES_acyltransf. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02946. acyl_WS_DGAT. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1."
    Kalscheuer R., Steinbuchel A.
    J. Biol. Chem. 278:8075-8082(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES, EXPRESSION IN OTHER BACTERIA, DISRUPTION PHENOTYPE.
  2. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiWSD_ACIAD
AccessioniPrimary (citable) accession number: Q8GGG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2003
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3