Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

O-acyltransferase WSD

Gene

wax-dgaT

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS uses C(12)-CoA to C(18)-CoA substrates whereas DGAT prefers C(20)-CoA. Upon expression in E.coli and Pseudomonas citronellolis (DSM 50332) both WS and DGAT activities increase.

Catalytic activityi

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Kineticsi

  1. KM=15.6 µM for palmitoyl-CoA (C(16), in wax synthase)1 Publication
  2. KM=21.1 µM for palmitoyl-CoA (diacylglycerol acyltransferase)1 Publication
  1. Vmax=212.8 pmol/min/mg enzyme (wax synthase)1 Publication
  2. Vmax=54.3 pmol/min/mg enzyme (diacylglycerol acyltransferase)1 Publication

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133Proton acceptorSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciASP62977:G1GJ8-770-MONOMER
MetaCyc:ACIAD0832-MONOMER
BRENDAi2.3.1.20 8909
2.3.1.75 95
UniPathwayiUPA00282

Names & Taxonomyi

Protein namesi
Recommended name:
O-acyltransferase WSD
Alternative name(s):
Diacylglycerol O-acyltransferase (EC:2.3.1.20)
Short name:
DGAT
Long-chain-alcohol O-fatty-acyltransferase (EC:2.3.1.75)
Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
Wax synthase
Short name:
WS
Gene namesi
Name:wax-dgaT
Ordered Locus Names:ACIAD0832
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
Proteomesi
  • UP000000430 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not make wax ester and only trace amounts of triacylglycerol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003933501 – 458O-acyltransferase WSDAdd BLAST458

Interactioni

Protein-protein interaction databases

STRINGi62977.ACIAD0832

Structurei

3D structure databases

ProteinModelPortaliQ8GGG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D8T Bacteria
ENOG410XS7A LUCA
HOGENOMiHOG000277728
KOiK00635
OMAiFQPTFRK
OrthoDBiPOG091H0AJ0

Family and domain databases

InterProiView protein in InterPro
IPR014292 Acyl_transf_WS/DGAT
IPR009721 O-acyltransferase_WSD1_C
IPR004255 O-acyltransferase_WSD1_N
PfamiView protein in Pfam
PF06974 DUF1298, 1 hit
PF03007 WES_acyltransf, 1 hit
TIGRFAMsiTIGR02946 acyl_WS_DGAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GGG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLHPIDFI FLSLEKRQQP MHVGGLFLFQ IPDNAPDTFI QDLVNDIRIS
60 70 80 90 100
KSIPVPPFNN KLNGLFWDED EEFDLDHHFR HIALPHPGRI RELLIYISQE
110 120 130 140 150
HSTLLDRAKP LWTCNIIEGI EGNRFAMYFK IHHAMVDGVA GMRLIEKSLS
160 170 180 190 200
HDVTEKSIVP PWCVEGKRAK RLREPKTGKI KKIMSGIKSQ LQATPTVIQE
210 220 230 240 250
LSQTVFKDIG RNPDHVSSFQ APCSILNQRV SSSRRFAAQS FDLDRFRNIA
260 270 280 290 300
KSLNVTINDV VLAVCSGALR AYLMSHNSLP SKPLIAMVPA SIRNDDSDVS
310 320 330 340 350
NRITMILANL ATHKDDPLQR LEIIRRSVQN SKQRFKRMTS DQILNYSAVV
360 370 380 390 400
YGPAGLNIIS GMMPKRQAFN LVISNVPGPR EPLYWNGAKL DALYPASIVL
410 420 430 440 450
DGQALNITMT SYLDKLEVGL IACRNALPRM QNLLTHLEEE IQLFEGVIAK

QEDIKTAN
Length:458
Mass (Da):51,780
Last modified:March 1, 2003 - v1
Checksum:iAEFB9A1C83FE3823
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF529086 Genomic DNA Translation: AAO17391.1
CR543861 Genomic DNA Translation: CAG67733.1
RefSeqiWP_004922247.1, NC_005966.1

Genome annotation databases

EnsemblBacteriaiCAG67733; CAG67733; ACIAD0832
KEGGiaci:ACIAD0832

Similar proteinsi

Entry informationi

Entry nameiWSD_ACIAD
AccessioniPrimary (citable) accession number: Q8GGG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health