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Protein

Thiazole synthase

Gene

thiG

Organism
Erwinia pyrifoliae (strain Ep1/96)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Schiff-base intermediate with DXPUniRule annotation1
Binding sitei152DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciEPYR634499:G1GJ0-3876-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:EpC_pEp360130
Encoded oniPlasmid pEP360 Publication
OrganismiErwinia pyrifoliae (strain Ep1/96)
Taxonomic identifieri634499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeErwinia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001628201 – 252Thiazole synthaseAdd BLAST252

Proteomic databases

PRIDEiQ8GEI4

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8GEI4
SMRiQ8GEI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 180DXP bindingUniRule annotation2
Regioni201 – 202DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GEI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYDFVPQSR FLLGTAGYPS PQILQQAVMA SESEIITVSL RREGSQGGAF
60 70 80 90 100
RELLTQLNKR ILPNTAGCHT VKEAVTTAHM ARELFNTRWI KLEVIGHADT
110 120 130 140 150
LQPDPFALVE AARILCADGF QVFPYTTEDL ILGEKLLEAG CELLMPWGAP
160 170 180 190 200
IGSGQGLRNI EGLRSMRLWF KDIPLIIDAG IGAPSQAAQA MEMGFDGILL
210 220 230 240 250
NTAVARAQDP LRMAQAFAAA VRAGYDAHGA GLIERRDMAT ASTPIFGMAQ

FS
Length:252
Mass (Da):27,355
Last modified:February 8, 2011 - v2
Checksum:i561772A042A9DCDB
GO

Sequence cautioni

The sequence AAN04534 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123045 Genomic DNA Translation: AAN04534.1 Different initiation.
FP236829 Genomic DNA Translation: CAX53415.1
RefSeqiNP_758746.1, NC_004445.1
WP_011078061.1, NC_004445.1
WP_012814638.1, NC_013263.1

Genome annotation databases

EnsemblBacteriaiCAX53415; CAX53415; EpC_pEp360130
GeneIDi1047230
KEGGiepy:EpC_pEp360130

Similar proteinsi

Entry informationi

Entry nameiTHIG_ERWPE
AccessioniPrimary (citable) accession number: Q8GEI4
Secondary accession number(s): C8ZLR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: February 8, 2011
Last modified: May 23, 2018
This is version 88 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

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