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Protein

Thiazole synthase

Gene

thiG

Organism
Erwinia pyrifoliae (strain Ep1/96)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911Schiff-base intermediate with DXPUniRule annotation
Binding sitei152 – 1521DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciEPYR634499:GJIP-3812-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:EpC_pEp360130
Encoded oniPlasmid pEP360 Publication
OrganismiErwinia pyrifoliae (strain Ep1/96)
Taxonomic identifieri634499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Thiazole synthasePRO_0000162820Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8GEI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 1802DXP bindingUniRule annotation
Regioni201 – 2022DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiEOG6KMBD9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GEI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYDFVPQSR FLLGTAGYPS PQILQQAVMA SESEIITVSL RREGSQGGAF
60 70 80 90 100
RELLTQLNKR ILPNTAGCHT VKEAVTTAHM ARELFNTRWI KLEVIGHADT
110 120 130 140 150
LQPDPFALVE AARILCADGF QVFPYTTEDL ILGEKLLEAG CELLMPWGAP
160 170 180 190 200
IGSGQGLRNI EGLRSMRLWF KDIPLIIDAG IGAPSQAAQA MEMGFDGILL
210 220 230 240 250
NTAVARAQDP LRMAQAFAAA VRAGYDAHGA GLIERRDMAT ASTPIFGMAQ

FS
Length:252
Mass (Da):27,355
Last modified:February 8, 2011 - v2
Checksum:i561772A042A9DCDB
GO

Sequence cautioni

The sequence AAN04534.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123045 Genomic DNA. Translation: AAN04534.1. Different initiation.
FP236829 Genomic DNA. Translation: CAX53415.1.
RefSeqiNP_758746.1. NC_004445.1.
WP_011078061.1. NC_004445.1.
WP_012814638.1. NC_013263.1.

Genome annotation databases

EnsemblBacteriaiCAX53415; CAX53415; EpC_pEp360130.
GeneIDi1047230.
KEGGiepy:EpC_pEp360130.
pg:1047230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123045 Genomic DNA. Translation: AAN04534.1. Different initiation.
FP236829 Genomic DNA. Translation: CAX53415.1.
RefSeqiNP_758746.1. NC_004445.1.
WP_011078061.1. NC_004445.1.
WP_012814638.1. NC_013263.1.

3D structure databases

ProteinModelPortaliQ8GEI4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX53415; CAX53415; EpC_pEp360130.
GeneIDi1047230.
KEGGiepy:EpC_pEp360130.
pg:1047230.

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiEOG6KMBD9.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciEPYR634499:GJIP-3812-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Relatedness of chromosomal and plasmid DNAs of Erwinia pyrifoliae and Erwinia amylovora."
    McGhee G.C., Schnabel E.L., Maxson-Stein K., Jones B., Stromberg V.K., Lacy G.H., Jones A.L.
    Appl. Environ. Microbiol. 68:6182-6192(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Ep1/96.
  2. "Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae."
    Kube M., Migdoll A.M., Gehring I., Heitmann K., Mayer Y., Kuhl H., Knaust F., Geider K., Reinhardt R.
    BMC Genomics 11:393-393(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ep1/96.

Entry informationi

Entry nameiTHIG_ERWPE
AccessioniPrimary (citable) accession number: Q8GEI4
Secondary accession number(s): C8ZLR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: February 8, 2011
Last modified: April 13, 2016
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.