SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8GEA9

- BGAL1_THESP

UniProt

Q8GEA9 - BGAL1_THESP

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-galactosidase BgaA

Gene
bgaA
Organism
Thermus sp.
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and p-nitrophenyl-beta-D-galactoside (pNPGal).1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Kineticsi

  1. KM=0.41 mM for pNPGal (at 70 degrees Celsius and pH 7.0)1 Publication
  2. KM=42 mM for lactose (at 70 degrees Celsius and pH 7.0)

Vmax=140 µmol/min/mg enzyme with pNPGal as substrate (at 70 degrees Celsius and pH 7.0)

Vmax=8.5 µmol/min/mg enzyme with lactose as substrate (at 70 degrees Celsius and pH 7.0)

pH dependencei

Optimum pH is around 5.0-6.0. Retains more than 80% of activity at pH range between 4.5 and 6.5. Retains 10% of activity at pH 4.0.

Temperature dependencei

Optimum temperature is 90 degrees Celsius. Retains 90% of activity even at 95 degrees Celsius. Retains 80% of activity during a 12-hour period of incubation at 70 degrees Celsius. Half-lives at 80 and 90 degrees Celsius are 23 and 4.7 hours, respectively.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021Substrate By similarity
Metal bindingi106 – 1061Zinc By similarity
Binding sitei140 – 1401Substrate By similarity
Active sitei141 – 1411Proton donor By similarity
Metal bindingi150 – 1501Zinc By similarity
Metal bindingi152 – 1521Zinc By similarity
Metal bindingi155 – 1551Zinc By similarity
Active sitei312 – 3121Nucleophile By similarity
Binding sitei320 – 3201Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaA (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:bgaA
OrganismiThermus sp.
Taxonomic identifieri275 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Beta-galactosidase BgaAPRO_0000407695Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8GEA9.
SMRiQ8GEA9. Positions 1-644.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 3634Substrate binding

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GEA9-1 [UniParc]FASTAAdd to Basket

« Hide

MLGVCYYPEH WPKARWKEDA RRMREAGLSY VRVGEFAWAL LEPEPGRLEW    50
GWLDEALATL AAEGLKVVLG TPTATPPKWL VDRYPEVLPV DREGRRRRFG 100
GRRHYCFSSP AYREEARRIV TLLAERYGGL EAVAGFQTDN EYGCHGTVRC 150
YCPRCQEAFR GWLKARYGTI EALNEAWGTA FWSQRYRNFT EVELPHLTVA 200
EPNPSHLLDY YRFASDQVRA FNRLQVEILR AHAPGKFITH NFMGFFTDLD 250
AFALAQDLDF ASWDSYPLGF TDLMPLPPEE KLRYARTGHP DVAAFHHDLY 300
RGVGRGRFWV MEQQPGPVNW APHNPSPTPG MVRLWTWEAL AHGAEVVSYF 350
RWRQAPFAQE QMHAGLHRPD SAPDQGFFEA KQVAEELAAL ALPPVAQAPV 400
ALVFDYEAAW VYEVQPQGAE WSYLGLIYLF YSALRRLGLD VDVVPPGASL 450
RGYALTVVPS LPIVRGEALK AFQEAEGIVL FGPRSGSKTE TFQIPRELPP 500
GPLQALLPLK VVRVESLPPG LLEVAEGPMG RFSLGLWREW VESPLRPWLA 550
FADGGGALYR EGRYLYLAAW PSPELLGVLL AGLAQEAGLR PVFLPEGLRL 600
RRRGPWVFAF NYGPEAVEAP APEGARFLLG GKRVGPYDLA VWEEA 645
Length:645
Mass (Da):72,714
Last modified:March 1, 2003 - v1
Checksum:iE1F13D0D598857BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY130259 Genomic DNA. Translation: AAN05443.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY130259 Genomic DNA. Translation: AAN05443.1 .

3D structure databases

ProteinModelPortali Q8GEA9.
SMRi Q8GEA9. Positions 1-644.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization."
    Kang S.K., Cho K.K., Ahn J.K., Bok J.D., Kang S.H., Woo J.H., Lee H.G., You S.K., Choi Y.J.
    J. Biotechnol. 116:337-346(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 43815 / IB-21.

Entry informationi

Entry nameiBGAL1_THESP
AccessioniPrimary (citable) accession number: Q8GEA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi