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Q8GEA9

- BGAL1_THESP

UniProt

Q8GEA9 - BGAL1_THESP

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Protein

Beta-galactosidase BgaA

Gene

bgaA

Organism
Thermus sp.
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and p-nitrophenyl-beta-D-galactoside (pNPGal).1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Kineticsi

  1. KM=0.41 mM for pNPGal (at 70 degrees Celsius and pH 7.0)1 Publication
  2. KM=42 mM for lactose (at 70 degrees Celsius and pH 7.0)1 Publication

Vmax=140 µmol/min/mg enzyme with pNPGal as substrate (at 70 degrees Celsius and pH 7.0)1 Publication

Vmax=8.5 µmol/min/mg enzyme with lactose as substrate (at 70 degrees Celsius and pH 7.0)1 Publication

pH dependencei

Optimum pH is around 5.0-6.0. Retains more than 80% of activity at pH range between 4.5 and 6.5. Retains 10% of activity at pH 4.0.1 Publication

Temperature dependencei

Optimum temperature is 90 degrees Celsius. Retains 90% of activity even at 95 degrees Celsius. Retains 80% of activity during a 12-hour period of incubation at 70 degrees Celsius. Half-lives at 80 and 90 degrees Celsius are 23 and 4.7 hours, respectively.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021SubstrateBy similarity
Metal bindingi106 – 1061ZincBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Active sitei141 – 1411Proton donorBy similarity
Metal bindingi150 – 1501ZincBy similarity
Metal bindingi152 – 1521ZincBy similarity
Metal bindingi155 – 1551ZincBy similarity
Active sitei312 – 3121NucleophileBy similarity
Binding sitei320 – 3201SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaA (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:bgaA1 Publication
OrganismiThermus sp.
Taxonomic identifieri275 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Beta-galactosidase BgaAPRO_0000407695Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8GEA9.
SMRiQ8GEA9. Positions 1-644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 3634Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GEA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGVCYYPEH WPKARWKEDA RRMREAGLSY VRVGEFAWAL LEPEPGRLEW
60 70 80 90 100
GWLDEALATL AAEGLKVVLG TPTATPPKWL VDRYPEVLPV DREGRRRRFG
110 120 130 140 150
GRRHYCFSSP AYREEARRIV TLLAERYGGL EAVAGFQTDN EYGCHGTVRC
160 170 180 190 200
YCPRCQEAFR GWLKARYGTI EALNEAWGTA FWSQRYRNFT EVELPHLTVA
210 220 230 240 250
EPNPSHLLDY YRFASDQVRA FNRLQVEILR AHAPGKFITH NFMGFFTDLD
260 270 280 290 300
AFALAQDLDF ASWDSYPLGF TDLMPLPPEE KLRYARTGHP DVAAFHHDLY
310 320 330 340 350
RGVGRGRFWV MEQQPGPVNW APHNPSPTPG MVRLWTWEAL AHGAEVVSYF
360 370 380 390 400
RWRQAPFAQE QMHAGLHRPD SAPDQGFFEA KQVAEELAAL ALPPVAQAPV
410 420 430 440 450
ALVFDYEAAW VYEVQPQGAE WSYLGLIYLF YSALRRLGLD VDVVPPGASL
460 470 480 490 500
RGYALTVVPS LPIVRGEALK AFQEAEGIVL FGPRSGSKTE TFQIPRELPP
510 520 530 540 550
GPLQALLPLK VVRVESLPPG LLEVAEGPMG RFSLGLWREW VESPLRPWLA
560 570 580 590 600
FADGGGALYR EGRYLYLAAW PSPELLGVLL AGLAQEAGLR PVFLPEGLRL
610 620 630 640
RRRGPWVFAF NYGPEAVEAP APEGARFLLG GKRVGPYDLA VWEEA
Length:645
Mass (Da):72,714
Last modified:March 1, 2003 - v1
Checksum:iE1F13D0D598857BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY130259 Genomic DNA. Translation: AAN05443.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY130259 Genomic DNA. Translation: AAN05443.1 .

3D structure databases

ProteinModelPortali Q8GEA9.
SMRi Q8GEA9. Positions 1-644.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization."
    Kang S.K., Cho K.K., Ahn J.K., Bok J.D., Kang S.H., Woo J.H., Lee H.G., You S.K., Choi Y.J.
    J. Biotechnol. 116:337-346(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 43815 / IB-21Imported.

Entry informationi

Entry nameiBGAL1_THESP
AccessioniPrimary (citable) accession number: Q8GEA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 1, 2003
Last modified: October 1, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3