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Q8GEA9

- BGAL1_THESP

UniProt

Q8GEA9 - BGAL1_THESP

Protein

Beta-galactosidase BgaA

Gene

bgaA

Organism
Thermus sp.
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and p-nitrophenyl-beta-D-galactoside (pNPGal).1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Kineticsi

    1. KM=0.41 mM for pNPGal (at 70 degrees Celsius and pH 7.0)1 Publication
    2. KM=42 mM for lactose (at 70 degrees Celsius and pH 7.0)1 Publication

    Vmax=140 µmol/min/mg enzyme with pNPGal as substrate (at 70 degrees Celsius and pH 7.0)1 Publication

    Vmax=8.5 µmol/min/mg enzyme with lactose as substrate (at 70 degrees Celsius and pH 7.0)1 Publication

    pH dependencei

    Optimum pH is around 5.0-6.0. Retains more than 80% of activity at pH range between 4.5 and 6.5. Retains 10% of activity at pH 4.0.1 Publication

    Temperature dependencei

    Optimum temperature is 90 degrees Celsius. Retains 90% of activity even at 95 degrees Celsius. Retains 80% of activity during a 12-hour period of incubation at 70 degrees Celsius. Half-lives at 80 and 90 degrees Celsius are 23 and 4.7 hours, respectively.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei102 – 1021SubstrateBy similarity
    Metal bindingi106 – 1061ZincBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Active sitei141 – 1411Proton donorBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Metal bindingi152 – 1521ZincBy similarity
    Metal bindingi155 – 1551ZincBy similarity
    Active sitei312 – 3121NucleophileBy similarity
    Binding sitei320 – 3201SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaA (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:bgaA1 Publication
    OrganismiThermus sp.
    Taxonomic identifieri275 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 645645Beta-galactosidase BgaAPRO_0000407695Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GEA9.
    SMRiQ8GEA9. Positions 1-644.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni360 – 3634Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR013780. Glyco_hydro_13_b.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8GEA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGVCYYPEH WPKARWKEDA RRMREAGLSY VRVGEFAWAL LEPEPGRLEW    50
    GWLDEALATL AAEGLKVVLG TPTATPPKWL VDRYPEVLPV DREGRRRRFG 100
    GRRHYCFSSP AYREEARRIV TLLAERYGGL EAVAGFQTDN EYGCHGTVRC 150
    YCPRCQEAFR GWLKARYGTI EALNEAWGTA FWSQRYRNFT EVELPHLTVA 200
    EPNPSHLLDY YRFASDQVRA FNRLQVEILR AHAPGKFITH NFMGFFTDLD 250
    AFALAQDLDF ASWDSYPLGF TDLMPLPPEE KLRYARTGHP DVAAFHHDLY 300
    RGVGRGRFWV MEQQPGPVNW APHNPSPTPG MVRLWTWEAL AHGAEVVSYF 350
    RWRQAPFAQE QMHAGLHRPD SAPDQGFFEA KQVAEELAAL ALPPVAQAPV 400
    ALVFDYEAAW VYEVQPQGAE WSYLGLIYLF YSALRRLGLD VDVVPPGASL 450
    RGYALTVVPS LPIVRGEALK AFQEAEGIVL FGPRSGSKTE TFQIPRELPP 500
    GPLQALLPLK VVRVESLPPG LLEVAEGPMG RFSLGLWREW VESPLRPWLA 550
    FADGGGALYR EGRYLYLAAW PSPELLGVLL AGLAQEAGLR PVFLPEGLRL 600
    RRRGPWVFAF NYGPEAVEAP APEGARFLLG GKRVGPYDLA VWEEA 645
    Length:645
    Mass (Da):72,714
    Last modified:March 1, 2003 - v1
    Checksum:iE1F13D0D598857BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY130259 Genomic DNA. Translation: AAN05443.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY130259 Genomic DNA. Translation: AAN05443.1 .

    3D structure databases

    ProteinModelPortali Q8GEA9.
    SMRi Q8GEA9. Positions 1-644.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR013780. Glyco_hydro_13_b.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization."
      Kang S.K., Cho K.K., Ahn J.K., Bok J.D., Kang S.H., Woo J.H., Lee H.G., You S.K., Choi Y.J.
      J. Biotechnol. 116:337-346(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 43815 / IB-21Imported.

    Entry informationi

    Entry nameiBGAL1_THESP
    AccessioniPrimary (citable) accession number: Q8GEA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3