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Protein

Carboxylic ester hydrolase

Gene
N/A
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Enzyme and pathway databases

SABIO-RKQ8GCC7.

Protein family/group databases

ESTHERigeost-est50. Carb_B_Bacteria.
MEROPSiS09.948.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxylic ester hydrolaseUniRule annotation (EC:3.1.1.-UniRule annotation)
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OGSX-ray2.02A1-498[»]
2OGTX-ray1.58A1-498[»]
ProteinModelPortaliQ8GCC7.
SMRiQ8GCC7. Positions 3-496.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GCC7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 481479COesteraseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type-B carboxylesterase/lipase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GCC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTVVETRY GRLRGEMNEG VFVWKGIPYA KAPVGERRFL PPEPPDAWDG
60 70 80 90 100
VREATSFGPV VMQPSDPIFS GLLGRMSEAP SEDGLYLNIW SPAADGKKRP
110 120 130 140 150
VLFWIHGGAF LFGSGSSPWY DGTAFAKHGD VVVVTINYRM NVFGFLHLGD
160 170 180 190 200
SFGEAYAQAG NLGILDQVAA LRWVKENIAA FGGDPDNITI FGESAGAASV
210 220 230 240 250
GVLLSLPEAS GLFRRAMLQS GSGSLLLRSP ETAMAMTERI LDKAGIRPGD
260 270 280 290 300
RERLLSIPAE ELLRAALSLG PGVMYGPVVD GRVLRRHPIE ALRYGAASGI
310 320 330 340 350
PILIGVTKDE YNLFTLTDPS WTKLGEKELL DRINREVGPV PEEAIRYYKE
360 370 380 390 400
TAEPSAPTWQ TWLRIMTYRV FVEGMLRTAD AQAAQGADVY MYRFDYETPV
410 420 430 440 450
FGGQLKACHA LELPFVFHNL HQPGVANFVG NRPEREAIAN EMHYAWLSFA
460 470 480 490
RTGDPNGAHL PEAWPAYTNE RKAAFVFSAA SHVEDDPFGR ERAAWQGR
Length:498
Mass (Da):54,865
Last modified:March 1, 2003 - v1
Checksum:i70EEF9936AC1D882
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186196 Genomic DNA. Translation: AAN81910.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186196 Genomic DNA. Translation: AAN81910.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OGSX-ray2.02A1-498[»]
2OGTX-ray1.58A1-498[»]
ProteinModelPortaliQ8GCC7.
SMRiQ8GCC7. Positions 3-496.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERigeost-est50. Carb_B_Bacteria.
MEROPSiS09.948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKQ8GCC7.

Miscellaneous databases

EvolutionaryTraceiQ8GCC7.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus."
    Ewis H.E., Abdelal A.T., Lu C.D.
    Gene 329:187-195(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 12980Imported.
  2. "Crystal structure of the Geobacillus stearothermophilus carboxylesterase Est55 and its activation of prodrug CPT-11."
    Liu P., Ewis H.E., Tai P.C., Lu C.D., Weber I.T.
    J. Mol. Biol. 367:212-223(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS).

Entry informationi

Entry nameiQ8GCC7_GEOSE
AccessioniPrimary (citable) accession number: Q8GCC7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.