Reviewed, UniProtKB/Swiss-Prot Q8GAW0 (CPNB_COMS9)

Names and origin

Protein names

Recommended name:
    Cyclopentanone 1,2-monooxygenase
      Short name=CPMO
    EC=1.14.13.16

Gene names

Name:

cpnB

Organism

Comamonas sp. (strain NCIMB 9872)

Taxonomic identifier

213664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cyclopentanone + NADPH + O2 = 5-valerolactone + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Alcohol metabolism; cyclopentanol degradation; 5-valerolactone from cyclopentanol: step 2/2.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the FAD-binding monooxygenase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cyclopentanone monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.3
	Chain	2 – 550	549	Cyclopentanone 1,2-monooxygenase		PRO_0000186453
Regions
	Nucleotide binding	31 – 32	2	FAD By similarity
Sites
	Binding site	51	1	FAD By similarity
	Binding site	60	1	FAD By similarity
	Binding site	71	1	FAD By similarity
	Binding site	77	1	FAD By similarity
	Binding site	123	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Site	344	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8GAW0-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8956EF95203F6173	FASTA	550	62,111
	10 20 30 40 50 60 MTTMTTMTTE QLGMNNSVND KLDVLLIGAG FTGLYQLYHL RKLGYKVHLV DAGADIGGIW 70 80 90 100 110 120 HWNCYPGARV DTHCQIYQYS IPELWQEFNW KELFPNWAQM REYFHFADKK LDLSKDISFN 130 140 150 160 170 180 TRVQSAVFDE GTREWTVRSI GHQPIQARFV IANLGFGASP STPNVDGIET FKGQWYHTAL 190 200 210 220 230 240 WPQEGVNMAG KRVAIIGTGS SGVQVAQEAA LDAKQVTVYQ RTPNLALPMH QKQLSAEDNL 250 260 270 280 290 300 RMKPELPAAF ERRGKCFAGF DFDFIAKNAT ELSAAERTEI LEELWNAGGF RYWLANFQDY 310 320 330 340 350 360 LFDDKANDYV YEFWRDKVRA RIKDPKVAEK LAPMKKPHPY GAKRPSLEQW YYEIFNQNNV 370 380 390 400 410 420 TLVDVNETPV LRITEKGIVT AEGEAEFDLI VFATGFDAVT GGLTSIDFRN NQGQSFKDVW 430 440 450 460 470 480 SDGIRTQLGV ATAGFPNLLF GYGPQSPAGF CNGPSSAEYQ GDLLIQLMNY LRDNNISRIE 490 500 510 520 530 540 AQSEAQEEWS KLIADFWDSS LFPRAKSWYQ GSNIPGKKVE SLNFPLGLPT YISKFNESAE 550 KGYAGFSLAS

References

[1]	"Cloning and characterization of a gene cluster involved in cyclopentanol metabolism in Comamonas sp. strain NCIMB 9872 and biotransformations effected by Escherichia coli-expressed cyclopentanone 1,2-monooxygenase." Iwaki H., Hasegawa Y., Wang S., Kayser M.M., Lau P.C.K. Appl. Environ. Microbiol. 68:5671-5684(2002) [PubMed: 12406764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-41.
[2]	Erratum Iwaki H., Hasegawa Y., Wang S., Kayser M.M., Lau P.C.K. Appl. Environ. Microbiol. 69:2414-2414(2003)
[3]	"Oxidative biotransformations by microorganisms: production of chiral synthons by cyclopentanone monooxygenase from Pseudomonas sp. NCIMB 9872." Bes M.T., Villa R., Roberts S.M., Wan P.W.H., Willetts A. J. Mol. Catal., B Enzym. 1:127-134(1996) Cited for: PROTEIN SEQUENCE OF 2-30, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB073151 Genomic DNA. Translation: BAC22652.1. AB022102 Genomic DNA. Translation: BAC01269.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CPNB_COMS9

Accession

Primary (citable) accession number: Q8GAW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 30 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)