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Q8G9L0

- Q8G9L0_RHOOP

UniProt

Q8G9L0 - Q8G9L0_RHOOP

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Protein

Muconolactone Delta-isomerase

Gene

clcF

Organism
Rhodococcus opacus (Nocardia opaca)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Chloride 1
Binding sitei52 – 521Chloride 2
Binding sitei52 – 521Chloride 3
Binding sitei62 – 621Chloride 6; via amide nitrogen
Binding sitei65 – 651Chloride 7Imported
Binding sitei65 – 651Chloride 8Imported
Binding sitei87 – 871Chloride 1Imported
Binding sitei87 – 871Chloride 2Imported
Binding sitei87 – 871Chloride 3Imported

GO - Molecular functioni

  1. muconolactone delta-isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotation

Keywords - Biological processi

Aromatic hydrocarbons catabolismUniRule annotation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14666.
UniPathwayiUPA00157; UER00260.

Names & Taxonomyi

Protein namesi
Recommended name:
Muconolactone Delta-isomeraseUniRule annotation (EC:5.3.3.4UniRule annotation)
Short name:
MIaseUniRule annotation
Gene namesi
Name:clcFImported
Encoded oniPlasmid p1CPImported
OrganismiRhodococcus opacus (Nocardia opaca)Imported
Taxonomic identifieri37919 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZNJX-ray2.101/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f1-94[»]
3ZNUX-ray1.65A/B/C/D/E/F/G/H/I/J1-94[»]
3ZO7X-ray2.22A/B/C/D/E/F/G/H/I/J1-94[»]
4FPIX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U1-94[»]
ProteinModelPortaliQ8G9L0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the muconolactone Delta-isomerase family.UniRule annotation

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view]
PfamiPF02426. MIase. 1 hit.
[Graphical view]
PIRSFiPIRSF001486. CatC. 1 hit.
ProDomiPD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54909. SSF54909. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G9L0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLYLVRMTVN LPRNLDPREE ERLKASEKAR SRTLQEQGQW RYLWRTTGKY
60 70 80 90
GNISVFDVNS HDELHEILWS LPFFPYLTID VEPLSHHPAR VGKD
Length:94
Mass (Da):11,194
Last modified:March 1, 2003 - v1
Checksum:i610E6289A5EE9F5B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ439407 Genomic DNA. Translation: CAD28145.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ439407 Genomic DNA. Translation: CAD28145.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ZNJ X-ray 2.10 1/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f 1-94 [» ]
3ZNU X-ray 1.65 A/B/C/D/E/F/G/H/I/J 1-94 [» ]
3ZO7 X-ray 2.22 A/B/C/D/E/F/G/H/I/J 1-94 [» ]
4FPI X-ray 2.20 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U 1-94 [» ]
ProteinModelPortali Q8G9L0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00157 ; UER00260 .
BioCyci MetaCyc:MONOMER-14666.

Family and domain databases

InterProi IPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view ]
Pfami PF02426. MIase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001486. CatC. 1 hit.
ProDomi PD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF54909. SSF54909. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence."
    Moiseeva O.V., Solyanikova I.P., Kaschabek S.R., Groening J., Thiel M., Golovleva L.A., Schloemann M.
    J. Bacteriol. 184:5282-5292(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 1CPImported.
    Plasmid: p1CP
  2. "Identification and expression analysis of three two-component (chloro)phenol hydroxylases in Rhodococcus opacus 1CP."
    Groening J.A.D., Eulberg D., Benndorf D., Kaschabek S.R., Archer J.A.C., Schloemann M.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 1CPImported.
    Plasmid: p1CP
  3. "X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP."
    Ferraroni M., Kolomytseva M., Golovleva L.A., Scozzafava A.
    J. Struct. Biol. 182:44-50(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
  4. "Crystal structure and catalytic mechanism of chloromuconolactone dehalogenase ClcF from Rhodococcus opacus 1CP."
    Roth C., Groning J.A., Kaschabek S.R., Schlomann M., Strater N.
    Mol. Microbiol. 88:254-267(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Entry informationi

Entry nameiQ8G9L0_RHOOP
AccessioniPrimary (citable) accession number: Q8G9L0
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, PlasmidImported

External Data

Dasty 3