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Protein

Muconolactone Delta-isomerase

Gene

clcF

Organism
Rhodococcus opacus (Nocardia opaca)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. muconolactone delta-isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotation

Keywords - Biological processi

Aromatic hydrocarbons catabolismUniRule annotation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14666.
UniPathwayiUPA00157; UER00260.

Names & Taxonomyi

Protein namesi
Recommended name:
Muconolactone Delta-isomeraseUniRule annotation (EC:5.3.3.4UniRule annotation)
Short name:
MIaseUniRule annotation
Gene namesi
Name:clcFImported
Encoded oniPlasmid p1CPImported
OrganismiRhodococcus opacus (Nocardia opaca)Imported
Taxonomic identifieri37919 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZNJX-ray2.101/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f1-94[»]
3ZNUX-ray1.65A/B/C/D/E/F/G/H/I/J1-94[»]
3ZO7X-ray2.22A/B/C/D/E/F/G/H/I/J1-94[»]
4FPIX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U1-94[»]
ProteinModelPortaliQ8G9L0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the muconolactone Delta-isomerase family.UniRule annotation

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view]
PfamiPF02426. MIase. 1 hit.
[Graphical view]
PIRSFiPIRSF001486. CatC. 1 hit.
ProDomiPD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54909. SSF54909. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G9L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYLVRMTVN LPRNLDPREE ERLKASEKAR SRTLQEQGQW RYLWRTTGKY
60 70 80 90
GNISVFDVNS HDELHEILWS LPFFPYLTID VEPLSHHPAR VGKD
Length:94
Mass (Da):11,194
Last modified:March 1, 2003 - v1
Checksum:i610E6289A5EE9F5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ439407 Genomic DNA. Translation: CAD28145.1.
RefSeqiWP_032492678.1. NG_036922.1.
YP_009083241.1. NG_036922.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ439407 Genomic DNA. Translation: CAD28145.1.
RefSeqiWP_032492678.1. NG_036922.1.
YP_009083241.1. NG_036922.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZNJX-ray2.101/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f1-94[»]
3ZNUX-ray1.65A/B/C/D/E/F/G/H/I/J1-94[»]
3ZO7X-ray2.22A/B/C/D/E/F/G/H/I/J1-94[»]
4FPIX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U1-94[»]
ProteinModelPortaliQ8G9L0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00157; UER00260.
BioCyciMetaCyc:MONOMER-14666.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view]
PfamiPF02426. MIase. 1 hit.
[Graphical view]
PIRSFiPIRSF001486. CatC. 1 hit.
ProDomiPD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54909. SSF54909. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence."
    Moiseeva O.V., Solyanikova I.P., Kaschabek S.R., Groening J., Thiel M., Golovleva L.A., Schloemann M.
    J. Bacteriol. 184:5282-5292(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 1CPImported.
    Plasmid: p1CP
  2. "Identification and expression analysis of three two-component (chloro)phenol hydroxylases in Rhodococcus opacus 1CP."
    Groening J.A.D., Eulberg D., Benndorf D., Kaschabek S.R., Archer J.A.C., Schloemann M.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 1CPImported.
    Plasmid: p1CP
  3. "X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP."
    Ferraroni M., Kolomytseva M., Golovleva L.A., Scozzafava A.
    J. Struct. Biol. 182:44-50(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
  4. "Crystal structure and catalytic mechanism of chloromuconolactone dehalogenase ClcF from Rhodococcus opacus 1CP."
    Roth C., Groning J.A., Kaschabek S.R., Schlomann M., Strater N.
    Mol. Microbiol. 88:254-267(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Entry informationi

Entry nameiQ8G9L0_RHOOP
AccessioniPrimary (citable) accession number: Q8G9L0
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: February 4, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, PlasmidImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.