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Q8G9L0 (Q8G9L0_RHOOP) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Muconolactone Delta-isomerase PIRNR PIRNR001486

Short name=MIase PIRNR PIRNR001486
EC=5.3.3.4 PIRNR PIRNR001486
Gene names
Name:clcF EMBL CAD28145.1
OrganismRhodococcus opacus (Nocardia opaca) EMBL CAD28145.1
Taxonomic identifier37919 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Protein attributes

Sequence length94 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate. PIRNR PIRNR001486

Pathway

Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from catechol: step 3/3. PIRNR PIRNR001486

Sequence similarities

Belongs to the muconolactone Delta-isomerase family. PIRNR PIRNR001486

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism PIRNR PIRNR001486
   LigandChloride PDB 3ZNJ PDB 3ZNU PDB 3ZO7
   Molecular functionIsomerase PIRNR PIRNR001486
   Technical term3D-structure PDB 3ZNJ PDB 3ZNU PDB 3ZO7 PDB 4FPI
Gene Ontology (GO)
   Biological_processbeta-ketoadipate pathway

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmuconolactone delta-isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site521Chloride 1
Binding site521Chloride 2
Binding site521Chloride 3
Binding site621Chloride 6; via amide nitrogen
Binding site651Chloride 7 PDB 3ZO7
Binding site651Chloride 8 PDB 3ZO7
Binding site871Chloride 1 PDB 3ZNJ PDB 3ZNU
Binding site871Chloride 2 PDB 3ZNJ PDB 3ZNU
Binding site871Chloride 3 PDB 3ZNJ PDB 3ZNU

Sequences

Sequence LengthMass (Da)Tools
Q8G9L0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 610E6289A5EE9F5B

FASTA9411,194
        10         20         30         40         50         60 
MLYLVRMTVN LPRNLDPREE ERLKASEKAR SRTLQEQGQW RYLWRTTGKY GNISVFDVNS 

        70         80         90 
HDELHEILWS LPFFPYLTID VEPLSHHPAR VGKD 

« Hide

References

[1]"A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence."
Moiseeva O.V., Solyanikova I.P., Kaschabek S.R., Groning J., Thiel M., Golovleva L.A., Schlomann M.
J. Bacteriol. 184:5282-5292(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 1CP EMBL CAD28145.1.
[2]"X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP."
Ferraroni M., Kolomytseva M., Golovleva L.A., Scozzafava A.
J. Struct. Biol. 182:44-50(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
[3]"Crystal structure and catalytic mechanism of chloromuconolactone dehalogenase ClcF from Rhodococcus opacus 1CP."
Roth C., Groning J.A., Kaschabek S.R., Schlomann M., Strater N.
Mol. Microbiol. 88:254-267(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH CHLORIDE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ439407 Genomic DNA. Translation: CAD28145.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZNJX-ray2.101/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f1-94[»]
3ZNUX-ray1.65A/B/C/D/E/F/G/H/I/J1-94[»]
3ZO7X-ray2.22A/B/C/D/E/F/G/H/I/J1-94[»]
4FPIX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U1-94[»]
ProteinModelPortalQ8G9L0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14666.
UniPathwayUPA00157; UER00260.

Family and domain databases

InterProIPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view]
PfamPF02426. MIase. 1 hit.
[Graphical view]
PIRSFPIRSF001486. CatC. 1 hit.
ProDomPD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF54909. SSF54909. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ8G9L0_RHOOP
AccessionPrimary (citable) accession number: Q8G9L0
Entry history
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)