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Q8G9L0

- Q8G9L0_RHOOP

UniProt

Q8G9L0 - Q8G9L0_RHOOP

Protein

Muconolactone Delta-isomerase

Gene

clcF

Organism
Rhodococcus opacus (Nocardia opaca)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    (S)-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521Chloride 1
    Binding sitei52 – 521Chloride 2
    Binding sitei52 – 521Chloride 3
    Binding sitei62 – 621Chloride 6; via amide nitrogen
    Binding sitei65 – 651Chloride 7Imported
    Binding sitei65 – 651Chloride 8Imported
    Binding sitei87 – 871Chloride 1Imported
    Binding sitei87 – 871Chloride 2Imported
    Binding sitei87 – 871Chloride 3Imported

    GO - Molecular functioni

    1. muconolactone delta-isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-ketoadipate pathway Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    IsomeraseUniRule annotation

    Keywords - Biological processi

    Aromatic hydrocarbons catabolismUniRule annotation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14666.
    UniPathwayiUPA00157; UER00260.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muconolactone Delta-isomeraseUniRule annotation (EC:5.3.3.4UniRule annotation)
    Short name:
    MIaseUniRule annotation
    Gene namesi
    Name:clcFImported
    Encoded oniPlasmid p1CPImported
    OrganismiRhodococcus opacus (Nocardia opaca)Imported
    Taxonomic identifieri37919 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZNJX-ray2.101/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f1-94[»]
    3ZNUX-ray1.65A/B/C/D/E/F/G/H/I/J1-94[»]
    3ZO7X-ray2.22A/B/C/D/E/F/G/H/I/J1-94[»]
    4FPIX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U1-94[»]
    ProteinModelPortaliQ8G9L0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the muconolactone Delta-isomerase family.UniRule annotation

    Family and domain databases

    InterProiIPR011008. Dimeric_a/b-barrel.
    IPR026029. MLI_dom.
    IPR003464. Muconolactone_d_Isoase.
    [Graphical view]
    PfamiPF02426. MIase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001486. CatC. 1 hit.
    ProDomiPD006619. Muconolactone_d_Isoase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF54909. SSF54909. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8G9L0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLYLVRMTVN LPRNLDPREE ERLKASEKAR SRTLQEQGQW RYLWRTTGKY   50
    GNISVFDVNS HDELHEILWS LPFFPYLTID VEPLSHHPAR VGKD 94
    Length:94
    Mass (Da):11,194
    Last modified:March 1, 2003 - v1
    Checksum:i610E6289A5EE9F5B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ439407 Genomic DNA. Translation: CAD28145.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ439407 Genomic DNA. Translation: CAD28145.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZNJ X-ray 2.10 1/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f 1-94 [» ]
    3ZNU X-ray 1.65 A/B/C/D/E/F/G/H/I/J 1-94 [» ]
    3ZO7 X-ray 2.22 A/B/C/D/E/F/G/H/I/J 1-94 [» ]
    4FPI X-ray 2.20 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S/T/U 1-94 [» ]
    ProteinModelPortali Q8G9L0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00157 ; UER00260 .
    BioCyci MetaCyc:MONOMER-14666.

    Family and domain databases

    InterProi IPR011008. Dimeric_a/b-barrel.
    IPR026029. MLI_dom.
    IPR003464. Muconolactone_d_Isoase.
    [Graphical view ]
    Pfami PF02426. MIase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001486. CatC. 1 hit.
    ProDomi PD006619. Muconolactone_d_Isoase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence."
      Moiseeva O.V., Solyanikova I.P., Kaschabek S.R., Groening J., Thiel M., Golovleva L.A., Schloemann M.
      J. Bacteriol. 184:5282-5292(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 1CPImported.
      Plasmid: p1CP
    2. "Identification and expression analysis of three two-component (chloro)phenol hydroxylases in Rhodococcus opacus 1CP."
      Groening J.A.D., Eulberg D., Benndorf D., Kaschabek S.R., Archer J.A.C., Schloemann M.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 1CPImported.
      Plasmid: p1CP
    3. "X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP."
      Ferraroni M., Kolomytseva M., Golovleva L.A., Scozzafava A.
      J. Struct. Biol. 182:44-50(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
    4. "Crystal structure and catalytic mechanism of chloromuconolactone dehalogenase ClcF from Rhodococcus opacus 1CP."
      Roth C., Groning J.A., Kaschabek S.R., Schlomann M., Strater N.
      Mol. Microbiol. 88:254-267(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

    Entry informationi

    Entry nameiQ8G9L0_RHOOP
    AccessioniPrimary (citable) accession number: Q8G9L0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 1, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, PlasmidImported

    External Data

    Dasty 3