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Q8G9C6 (TRMB_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:AZOSEA12950
ORF Names:c1A43
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum ›

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171287

Regions

Region237 – 2404Substrate binding Potential

Sites

Binding site911S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1661S-adenosyl-L-methionine By similarity
Binding site1701Substrate By similarity
Binding site2021Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q8G9C6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 0659D4B4511FD1C1

FASTA25928,575
        10         20         30         40         50         60 
MTFPSHNPPE TGHPSAAPDE ALPAAEAPVP GDPEARFSSR SIRSFVLRQG RMSVAQQRHL 

        70         80         90        100        110        120 
DETLPKVGIP YRVAPLDLDA AFGRAAPKIV EIGFGMGETT AKIAAALPDK DFLTIEVHGP 

       130        140        150        160        170        180 
GVGSLCKLIA EGVLDNVRIV QHDAVEVLRD MIPERALAGV HVFFPDPWHK KRHHKRRIIQ 

       190        200        210        220        230        240 
PDFVALIASR LAPGAYLHCA TDWEEYAQWM LEVLAAEPAL ENTADGYAPR PAYRPLTKFE 

       250 
NRGLKLGHGV WDLVFRRRG

« Hide

References

« Hide 'large scale' references
[1]"Genes involved in the anaerobic degradation of ethylbenzene in a denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Beck A., Widdel F., Reinhardt R.
Arch. Microbiol. 178:506-516(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR555306 Genomic DNA. Translation: CAI07420.1.
RefSeqYP_158321.1. NC_006513.1.

3D structure databases

ProteinModelPortalQ8G9C6.
ModBaseSearch...

Protein-protein interaction databases

STRING76114.c1A43.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI07420; CAI07420; c1A43.
GeneID3180480.
KEGGeba:c1A43.
PATRIC20968707. VBIAroAro98752_1314.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAFIHCATD.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycAARO76114:GJTA-1314-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_AROAE
AccessionPrimary (citable) accession number: Q8G9C6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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