Reviewed, UniProtKB/Swiss-Prot Q8G816 (CARA_BIFLO)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	BL0067

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112257
Regions
	Domain	207 – 405	199	Glutamine amidotransferase type-1
	Region	1 – 203	203	CPSase HAMAP MF_01209
Sites
	Active site	283	1	Nucleophile By similarity
	Active site	378	1	By similarity
	Active site	380	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G816-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EC2232518EFFFD48	FASTA	407	44,265
	10 20 30 40 50 60 MSQNESGTIA IPMYDKDDAV LVLEDGQVYV GKPYGALGET TGEIVFATGM TGYQETLTDP 70 80 90 100 110 120 SYDRQIVVQT FPHIGDTGVN SEDPESSRIW VAGYIVRDPS PNVSNWRAEG SLDDDLAKNG 130 140 150 160 170 180 IVGLSHIDTR KLVRHLRSAG VMRAGIFSGD ALTDQATGAL KTIEQLLEDV KNTPQMQGLS 190 200 210 220 230 240 LYDEVSTKET YTIEPCGEYE GKEPLYTVAA VDLGIKGMTP HRMAERGCRV HVVPSTITFA 250 260 270 280 290 300 EIENLNPDGV FFSNGPGDPE QAGPEIELLR QVLDAGYPFF GICFGNQLLG RALGFGTYKL 310 320 330 340 350 360 KFGHRGINQP VKDLTTGKVE VTAHNHGFAV DAPIGKQVDA PFENGKYGKV FVSHIDLNDD 370 380 390 400 VVEGLQCVDI PAFSVQYHPE AAAGPHDAAY LFDRFCELMK NNSKEGK

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN23933.1.
RefSeq	NP_695297.1.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1022933.
GenomeReviews	Gene locus BL0067 in contig AE014295_GR.
KEGG	blo:BL0067.
NMPDR	fig|206672.1.peg.66.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G816.
OMA	Q8G816. LFDGSNC.
Enzyme and pathway databases
BioCyc	BLON206672:BL0067-MON.
BRENDA	6.3.5.5. 39917.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BIFLO

Accession

Primary (citable) accession number: Q8G816

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)