Reviewed, UniProtKB/Swiss-Prot Q8G7I0 (RNPH_BIFLO)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BL0286

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Ribonuclease PH HAMAP MF_00564		PRO_0000139871

Sequences

	Sequence		Length	Mass (Da)
	Q8G7I0-1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 6108AAAACE749E1E	FASTA	248	27,101
	10 20 30 40 50 60 MLGNHQIIRA DGRKVDELRP VRITRHFTDA PEGSVLIECG NTRVMCTATF TPGVPRWRKD 70 80 90 100 110 120 SGLGWVTAEY SMLPRATAER TDRESVRGKI GGRTHEISRL IGRCLRGVID MKALGENQIQ 130 140 150 160 170 180 LDCDVLQADG GTRTASVTGA YVALVDAVNW AEKHRHIKSA SRVLKDYVSA VSVGVINGTP 190 200 210 220 230 240 MLDLPYIEDS QAMTDMNVAM TGSGTFIEIQ GTAEHRPFNR AELGTLLDLA EKGNKELQAA QRAALSLD

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24126.1. Different initiation.
RefSeq	NP_695490.1.
3D structure databases
HSSP	HSSP built from PDB template 1R6L based on UniProtKB P50597.
ModBase	Search...
Genome annotation databases
GeneID	1023283.
GenomeReviews	Gene locus BL0286 in contig AE014295_GR.
KEGG	blo:BL0286.
NMPDR	fig|206672.1.peg.259.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G7I0.
Enzyme and pathway databases
BioCyc	BLON206672:BL0286-MON.
BRENDA	2.7.7.56. 39917.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BIFLO

Accession

Primary (citable) accession number: Q8G7I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 5, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)