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Q8G7C4 (DDL_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:BL0345
OrganismBifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium ›

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity.

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177787

Regions

Domain205 – 424220ATP-grasp
Nucleotide binding237 – 29963ATP By similarity

Sites

Metal binding3781Magnesium or manganese 1 By similarity
Metal binding3911Magnesium or manganese 1 By similarity
Metal binding3911Magnesium or manganese 2 By similarity
Metal binding3931Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G7C4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D501772E024C8F46

FASTA42846,045
        10         20         30         40         50         60 
MRLLCAAFRA RFGPVGGAKH RLENGCSFNK RMVMAKKRVV VLYGGRADEH SISCISTAGV 

        70         80         90        100        110        120 
LGAMDTERFE PIPVGITKDG KWIINGEDPR GWNLDGGELP TVKITPESRP VMLDPSRGQD 

       130        140        150        160        170        180 
GFFIGEPSHI NSADSGFGTS FVSMSDPEMH HVLTSLGHVD AVLPVLHGPY GEDGTVQGLL 

       190        200        210        220        230        240 
EMMGVPYVGC GVFASAACMD KHYTKVVLDA AGIPTAPGVT VDARNFTAAD VLAEIEDAGL 

       250        260        270        280        290        300 
TYPLFVKPSR AGSSFGVTKV EKADDRETQQ DRLAAAIATA GEHDWKVLVE QGIDGREIEC 

       310        320        330        340        350        360 
AVLCPKAGDE PEASWPGEIV LDHQNDDQFY DFDSKYMDAS ASHVEVPANL PVSVLEDVRD 

       370        380        390        400        410        420 
VARRAFKAVD GAGLSRVDTF VTPDGTVMVN EINTMPGFTP ISMYPKAWDA TGVSYTELIT 


RLIEGVLR

« Hide

References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014295 Genomic DNA. Translation: AAN24184.1.
RefSeqNP_695548.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G7C4.
ModBaseSearch...

Protein-protein interaction databases

STRING206672.BL0345.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN24184; AAN24184; BL0345.
GeneID1023250.
KEGGblo:BL0345.
PATRIC21119251. VBIBifLon107831_1082.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBLON206672:GI1E-986-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 2 hits.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BIFLO
AccessionPrimary (citable) accession number: Q8G7C4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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