Reviewed, UniProtKB/Swiss-Prot Q8G7C4 (DDL_BIFLO)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Synonyms:	ddlA
Ordered Locus Names:	BL0345

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000177787
Regions
	Domain	205 – 424	220	ATP-grasp
	Nucleotide binding	237 – 299	63	ATP By similarity
Sites
	Metal binding	378	1	Magnesium or manganese 1 By similarity
	Metal binding	391	1	Magnesium or manganese 1 By similarity
	Metal binding	391	1	Magnesium or manganese 2 By similarity
	Metal binding	393	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G7C4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D501772E024C8F46	FASTA	428	46,045
	10 20 30 40 50 60 MRLLCAAFRA RFGPVGGAKH RLENGCSFNK RMVMAKKRVV VLYGGRADEH SISCISTAGV 70 80 90 100 110 120 LGAMDTERFE PIPVGITKDG KWIINGEDPR GWNLDGGELP TVKITPESRP VMLDPSRGQD 130 140 150 160 170 180 GFFIGEPSHI NSADSGFGTS FVSMSDPEMH HVLTSLGHVD AVLPVLHGPY GEDGTVQGLL 190 200 210 220 230 240 EMMGVPYVGC GVFASAACMD KHYTKVVLDA AGIPTAPGVT VDARNFTAAD VLAEIEDAGL 250 260 270 280 290 300 TYPLFVKPSR AGSSFGVTKV EKADDRETQQ DRLAAAIATA GEHDWKVLVE QGIDGREIEC 310 320 330 340 350 360 AVLCPKAGDE PEASWPGEIV LDHQNDDQFY DFDSKYMDAS ASHVEVPANL PVSVLEDVRD 370 380 390 400 410 420 VARRAFKAVD GAGLSRVDTF VTPDGTVMVN EINTMPGFTP ISMYPKAWDA TGVSYTELIT RLIEGVLR

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24184.1.
RefSeq	NP_695548.1.
3D structure databases
HSSP	HSSP built from PDB template 1E4E based on UniProtKB P25051.
ModBase	Search...
Genome annotation databases
GeneID	1023250.
GenomeReviews	Gene locus BL0345 in contig AE014295_GR.
KEGG	blo:BL0345.
NMPDR	fig|206672.1.peg.317.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G7C4.
OMA	Q8G7C4. VIVEAMI.
Enzyme and pathway databases
BioCyc	BLON206672:BL0345-MON.
BRENDA	6.3.2.4. 39917.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BIFLO

Accession

Primary (citable) accession number: Q8G7C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)