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Q8G709 (SYE_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:BL0469
OrganismBifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119515

Regions

Motif21 – 3111"HIGH" region HAMAP-Rule MF_00022
Motif265 – 2695"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2681ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G709 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 3FB6166D424431AE

FASTA50656,681
        10         20         30         40         50         60 
MTDAENTKPE LPKNVRVRFC PSPTGTPHVG MIRTALFNWA EARATGGTLI FRIEDTDAVR 

        70         80         90        100        110        120 
DSEESYNQIL ESLRWLGIDW DEGIDVGGPH GPYRQSERTA IYKDVAAKLL EAGYAYESFS 

       130        140        150        160        170        180 
TPEEIKERNL AAGRPAEFGY DGYDRNLTDE QKAAFRAEGR KPALRIKMPD EDIAFDDLIR 

       190        200        210        220        230        240 
GTIEFKAGSV PDYVIVRPNG DPLYTLTNPV DDAMMEVNVV LRGEDLLSST PRQIVLYRYL 

       250        260        270        280        290        300 
MELGIAKEMP LFGHMPYVMG QGNKKLSKRD PESNLFNHRD NGFIREGLLN YLALLGWSIA 

       310        320        330        340        350        360 
PDRDVFSMDE MTEKFDVRDV KANPARFDID KAISINAEHI RMLEPEDFLR RSVPYLHRDG 

       370        380        390        400        410        420 
VVSADNWDAL TDREREVLTA AAPLVQPRVR LLGEVAGMVG SLLSTEGYLE PADDAKKQLK 

       430        440        450        460        470        480 
DSAGEVLDKA IAALEAVDEA DWKTDNLHET LNKALVEEGG YKPRLAFGPV RVAMSGRRVS 

       490        500 
PPLFESMEIV GKPVSLARLK GLREHL 

« Hide

References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014295 Genomic DNA. Translation: AAN24300.1.
RefSeqNP_695664.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G709.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING206672.BL0469.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN24300; AAN24300; BL0469.
GeneID1023225.
KEGGblo:BL0469.
PATRIC21119527. VBIBifLon107831_1212.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAPEGMLNY.
OrthoDBEOG6DRPF7.
PhylomeDBQ8G709.

Enzyme and pathway databases

BioCycBLON206672:GI1E-1149-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_BIFLO
AccessionPrimary (citable) accession number: Q8G709
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries