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Q8G709

- SYE_BIFLO

UniProt

Q8G709 - SYE_BIFLO

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bifidobacterium longum (strain NCC 2705)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei268 – 2681ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBLON206672:GI1E-1149-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:BL0469
    OrganismiBifidobacterium longum (strain NCC 2705)
    Taxonomic identifieri206672 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
    ProteomesiUP000000439: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506Glutamate--tRNA ligasePRO_0000119515Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi206672.BL0469.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8G709.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi21 – 3111"HIGH" regionAdd
    BLAST
    Motifi265 – 2695"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiPEGMLNY.
    OrthoDBiEOG6DRPF7.
    PhylomeDBiQ8G709.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8G709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDAENTKPE LPKNVRVRFC PSPTGTPHVG MIRTALFNWA EARATGGTLI    50
    FRIEDTDAVR DSEESYNQIL ESLRWLGIDW DEGIDVGGPH GPYRQSERTA 100
    IYKDVAAKLL EAGYAYESFS TPEEIKERNL AAGRPAEFGY DGYDRNLTDE 150
    QKAAFRAEGR KPALRIKMPD EDIAFDDLIR GTIEFKAGSV PDYVIVRPNG 200
    DPLYTLTNPV DDAMMEVNVV LRGEDLLSST PRQIVLYRYL MELGIAKEMP 250
    LFGHMPYVMG QGNKKLSKRD PESNLFNHRD NGFIREGLLN YLALLGWSIA 300
    PDRDVFSMDE MTEKFDVRDV KANPARFDID KAISINAEHI RMLEPEDFLR 350
    RSVPYLHRDG VVSADNWDAL TDREREVLTA AAPLVQPRVR LLGEVAGMVG 400
    SLLSTEGYLE PADDAKKQLK DSAGEVLDKA IAALEAVDEA DWKTDNLHET 450
    LNKALVEEGG YKPRLAFGPV RVAMSGRRVS PPLFESMEIV GKPVSLARLK 500
    GLREHL 506
    Length:506
    Mass (Da):56,681
    Last modified:March 1, 2003 - v1
    Checksum:i3FB6166D424431AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014295 Genomic DNA. Translation: AAN24300.1.
    RefSeqiNP_695664.1. NC_004307.2.

    Genome annotation databases

    EnsemblBacteriaiAAN24300; AAN24300; BL0469.
    GeneIDi1023225.
    KEGGiblo:BL0469.
    PATRICi21119527. VBIBifLon107831_1212.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014295 Genomic DNA. Translation: AAN24300.1 .
    RefSeqi NP_695664.1. NC_004307.2.

    3D structure databases

    ProteinModelPortali Q8G709.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 206672.BL0469.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN24300 ; AAN24300 ; BL0469 .
    GeneIDi 1023225.
    KEGGi blo:BL0469.
    PATRICi 21119527. VBIBifLon107831_1212.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi PEGMLNY.
    OrthoDBi EOG6DRPF7.
    PhylomeDBi Q8G709.

    Enzyme and pathway databases

    BioCyci BLON206672:GI1E-1149-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
      Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
      Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCC 2705.

    Entry informationi

    Entry nameiSYE_BIFLO
    AccessioniPrimary (citable) accession number: Q8G709
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3