Q8G700 (FTHS_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	BL0478

Organism

Bifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium longum

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199336

Sequences

	Sequence		Length	Mass (Da)
	Q8G700 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B5643626B9884BC8	FASTA	505	54,028
	10 20 30 40 50 60 MTTIEDFTSQ YGLVQKIDAF GYMKYLAENP DAPRKHGKVV LVTADTPLKA SRGEGKTTTT 70 80 90 100 110 120 IALIDALNKR GIDATAVLRQ PSMGITAAGS KGGASGGGKA SLTHPELIDW GLCGEMGAIE 130 140 150 160 170 180 AAQNLLVSFA EKAIDDGKLD TILVPRVSEV PSRSLRSTAV DYGKGNVPEK VVLTPTCELM 190 200 210 220 230 240 QIVVLSRSMD EISERVSKMI AGTKDGKAVT FGEFVDLWRI TGILADAVKP AKTETVNGSP 250 260 270 280 290 300 VYVHGGPFAN VSIGIPTLVS VEMACALHDV VIVEAGYGTD AGAQKWLDIA CREYGAQWPS 310 320 330 340 350 360 AAIVVTRAST WRDDPELAWR YPFHVQRLEN LDIPTFPLIN LWEGEDDQVP SLKATADELG 370 380 390 400 410 420 FRTPIIGNLF RDGGEALAPQ LDGFVDALQN GSMPAEPHSH KGMALTENAK WVAENAYGVP 430 440 450 460 470 480 AERVIYKPGF TESVSEAMEL CQSAGISLDD LAFVAVKSPA TMTDNDRAPE EERTVALKKV 490 500 EVHAGAGLVH VNLTTSLTTP MPKIV

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24309.1.
RefSeq	NP_695673.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G700.
ModBase	Search...
Protein-protein interaction databases
STRING	206672.BL0478.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN24309; AAN24309; BL0478.
GeneID	1022163.
KEGG	blo:BL0478.
PATRIC	21119545. VBIBifLon107831_1221.
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000112304.
KO	K01938.
OMA	KRSEENG.
ProtClustDB	CLSK572650.
Enzyme and pathway databases
BioCyc	BLON206672:GI1E-1110-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS. Divergent sequence.
InterPro	IPR027000. FHS_archaeal_bac. IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
PANTHER	PTHR10025:SF20. PTHR10025:SF20. 1 hit.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_BIFLO

Accession

Primary (citable) accession number: Q8G700

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program