Reviewed, UniProtKB/Swiss-Prot Q8G6V2 (ILVC1_BIFLO)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase 1
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 1
    Alpha-keto-beta-hydroxylacil reductoisomerase 1

Gene names

Name:	ilvC1
Ordered Locus Names:	BL0530

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Ketol-acid reductoisomerase 1 HAMAP MF_00435		PRO_0000151278
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G6V2-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: FD3E3B700BF65247	FASTA	350	38,560
	10 20 30 40 50 60 MAAQIWYEND GDLSVLEGKK VAIIGYGSQG HAHALNLRDS GVDVVVGLRP TSKSVDFAKE 70 80 90 100 110 120 QGLEVKSVAE ASAEADVIMI LAPDQYQRTI WANDIEPNIK PGAAVAFAHG FNIHYGYIKP 130 140 150 160 170 180 SEDHPVFMVA PKGPGHIVRR EYAAGRGVPV VVAVEQDPRG DGWALTLAYA KALGALRAGA 190 200 210 220 230 240 IKTTFKEETE TDLFGEQNVL MGGVNKLVEM GFEVLTDAGY QPEIAYFEVC HELKMLVDLM 250 260 270 280 290 300 NEGGLNKARW SCSDTAQYGD YVNTVINEDC RKRMEYHLQR IQDGSFAKEF IDDQDAGAPH 310 320 330 340 350 FKELQEKYSN ERIETVGPKL RAMFSWNKDG VKDADEANSF TGKIARAQVQ

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24357.1. Different initiation.
RefSeq	NP_695721.1.
3D structure databases
SMR	Q8G6V2. Positions 4-326.
ModBase	Search...
Genome annotation databases
GeneID	1023093.
GenomeReviews	Gene locus BL0530 in contig AE014295_GR.
KEGG	blo:BL0530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286525.
OMA	DSISNTA.
PhylomeDB	Q8G6V2.
Enzyme and pathway databases
BioCyc	BLON206672:BL0530-MONOMER.
BRENDA	1.1.1.86. 39917.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC1_BIFLO

Accession

Primary (citable) accession number: Q8G6V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)