Q8G6V2 (ILVC1_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase 1
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase 1
Alpha-keto-beta-hydroxylacil reductoisomerase 1

Gene names

Name:	ilvC1
Ordered Locus Names:	BL0530

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.
Sequence caution	The sequence AAN24357.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Ketol-acid reductoisomerase 1 HAMAP MF_00435		PRO_0000151278
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G6V2 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: FD3E3B700BF65247	FASTA	350	38,560
	10 20 30 40 50 60 MAAQIWYEND GDLSVLEGKK VAIIGYGSQG HAHALNLRDS GVDVVVGLRP TSKSVDFAKE 70 80 90 100 110 120 QGLEVKSVAE ASAEADVIMI LAPDQYQRTI WANDIEPNIK PGAAVAFAHG FNIHYGYIKP 130 140 150 160 170 180 SEDHPVFMVA PKGPGHIVRR EYAAGRGVPV VVAVEQDPRG DGWALTLAYA KALGALRAGA 190 200 210 220 230 240 IKTTFKEETE TDLFGEQNVL MGGVNKLVEM GFEVLTDAGY QPEIAYFEVC HELKMLVDLM 250 260 270 280 290 300 NEGGLNKARW SCSDTAQYGD YVNTVINEDC RKRMEYHLQR IQDGSFAKEF IDDQDAGAPH 310 320 330 340 350 FKELQEKYSN ERIETVGPKL RAMFSWNKDG VKDADEANSF TGKIARAQVQ

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24357.1. Different initiation.
RefSeq	NP_695721.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G6V2.
SMR	Q8G6V2. Positions 4-326.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1023093.
GenomeReviews	Gene locus BL0530 in contig AE014295_GR.
KEGG	blo:BL0530.
PATRIC	21119643. VBIBifLon107831_1270.
Phylogenomic databases
HOGENOM	HBG286525.
OMA	MAYAKGI.
PhylomeDB	Q8G6V2.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	BLON206672:BL0530-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC1_BIFLO

Accession

Primary (citable) accession number: Q8G6V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program