Reviewed, UniProtKB/Swiss-Prot Q8G6V1 (ILVC2_BIFLO)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase 2
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 2
    Alpha-keto-beta-hydroxylacil reductoisomerase 2

Gene names

Name:	ilvC2
Ordered Locus Names:	BL0531

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Ketol-acid reductoisomerase 2 HAMAP MF_00435		PRO_0000151279
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G6V1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E91A4B60022CA63E	FASTA	350	38,552
	10 20 30 40 50 60 MAATIWYEKD ADLSVFDGKK VAILGYGSQG HAHALNLRDS GVDVVVGLRP TSKSVEFAKE 70 80 90 100 110 120 QGLEVKPVGE AVAEADVVMI LLPDQYQAAV YKKDVEPNLK PGAALAFAHG FNIHYGYIKP 130 140 150 160 170 180 SEDHPVFMVA PKGPGHIVRR EYAAGRGVPV VVAVEQDPDG KTWPLCLAYA KALGALRAGA 190 200 210 220 230 240 IKTTFTEETE TDLFGEQDVL MGGINHLCDL GFDVLTEAGY QPEIAYFEVF HELKMLVDLA 250 260 270 280 290 300 NEGGLNKARW SCSDTAQYGD YTSTVITEET KKRMQYQLKR IQDGSFAKEF MDDQAAGAPK 310 320 330 340 350 FKKLQEEYSH PHLETVGPKL RAMFSWNNAE AKDKDETESF NGKIARTQVQ

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24358.1.
RefSeq	NP_695722.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1021989.
GenomeReviews	Gene locus BL0531 in contig AE014295_GR.
KEGG	blo:BL0531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G6V1.
OMA	Q8G6V1. AHGFNIR.
Enzyme and pathway databases
BioCyc	BLON206672:BL0531-MON.
BRENDA	1.1.1.86. 39917.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC2_BIFLO

Accession

Primary (citable) accession number: Q8G6V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)