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Q8G6I4 (ISPE_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:BL0656
OrganismBifidobacterium longum (strain NCC 2705)
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3163164-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
PRO_0000189190

Regions

Nucleotide binding126 – 13611ATP Potential

Sites

Active site321 By similarity
Active site1681 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G6I4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: AEF623697DB7875B

FASTA31633,286
        10         20         30         40         50         60 
MSPVISHPRS AAARHQSDEL SPITITVDCP AKTNLTLEVG PAHDEWGGRH ELDTIYCAIG 

        70         80         90        100        110        120 
VYDTVTATAK QPGAGFSLEL EGAYLGDLAS SRSDMRRNHA VLALFAMAQA AEREPDVALT 

       130        140        150        160        170        180 
ITKRIPVGAG LGGGSADAAA TMLAVNRLWE LNWPIERLRT IAATLGADMP FCLTGGLAYG 

       190        200        210        220        230        240 
TGFGERITDI APGSRDELAL IEQGFSGEVL VGAYQSQLST PEVYHTFDIV GAAEGDRNHL 

       250        260        270        280        290        300 
QAAAISLHPR SGQAIDAATQ AGASHAFVSG SGPSVVAFAA DEAAAQRIIE VWRDTAVVDR 

       310 
IIRAKSPEHP NISVRQ 

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References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014295 Genomic DNA. Translation: AAN24478.1.
RefSeqNP_695842.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G6I4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1023299.
GenomeReviewsGene locus BL0656 in contig AE014295_GR.
KEGGblo:BL0656.
NMPDRfig|206672.1.peg.609.
PATRIC21119885. VBIBifLon107831_1387.

Phylogenomic databases

HOGENOMHBG734593.
OMACQLARMS.
PhylomeDBQ8G6I4.
ProtClustDBCLSK572735.

Enzyme and pathway databases

BioCycBLON206672:BL0656-MONOMER.

Family and domain databases

HAMAPMF_00061. IspE.
[Tree]
InterProIPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KOK00919.
PANTHERPTHR20861:SF2. IspE. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_BIFLO
AccessionPrimary (citable) accession number: Q8G6I4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families