Reviewed, UniProtKB/Swiss-Prot Q8G6C2 (SYT_BIFLO)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BL0724

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_0000100944
Regions
	Region	255 – 561	307	Catalytic HAMAP MF_00184
Sites
	Metal binding	360	1	Zinc; catalytic By similarity
	Metal binding	411	1	Zinc; catalytic By similarity
	Metal binding	538	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G6C2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3A7004F179094DCB	FASTA	677	76,835
	10 20 30 40 50 60 MAQATISITV NGEAKEVEAT TTGVELFAED KNIIAVKING ENRDLYTPLN DGDTVDPIAL 70 80 90 100 110 120 DSEDGLAIMR HSATHVMAQA VQEVYPNAKL GVGPVIKDGF YYDFQVDQPF TPNDLKDIEK 130 140 150 160 170 180 RMQRIIKSSQ SFRRRSVTEE EALKEEADQP FKIELIEDKE AHLDPAAATE ISEKELSFYD 190 200 210 220 230 240 NVDRDGNVVW KDLCRGPHLP NTRYIKAFKI ERSAAAYWRG SEKNPTMQRV YGTAWATKED 250 260 270 280 290 300 LKAYQTRLEE AAKRDHRKLG AEMDLFSFPD EIGPGLAVFH PKGAAVINAM EDYSREMHRK 310 320 330 340 350 360 HHYSFVQTPH ITKGGLYETS GHLHWYKDGM YPPMHLDEEK DADGNITKPG ADYYLKPMNC 370 380 390 400 410 420 PMHNLIFKSR QRSYRELPLR LFEFGTVYRY EKSGEVHGLT RVRGLTQDDS HIYCTREQMK 430 440 450 460 470 480 DELTSLLTFV LNLLKDFGLT DFYLELSTKD PNKYVGSDEI WEEATNTLAE VAKESNLELV 490 500 510 520 530 540 DDPCGAAFYG PKISVQARDA IGRTWQVSTI QLDFNLPERF QLEYIAKDGT HQRPVMIHRA 550 560 570 580 590 600 LFGSIERFFA VLLEHYAGAF PAWLAPVQVL GVPVADEFAP HLAGFVKSLE DEMVRCEIDY 610 620 630 640 650 660 SDDRFGKKIR NASKSKVPFI LIVGEEDMNN NAVSFRFRDG SQLNGVPVDT AREQILTVIK 670 KRVQVNSADD FNAAVAE

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24541.1.
RefSeq	NP_695905.1.
3D structure databases
HSSP	HSSP built from PDB template 1QF6 based on UniProtKB P00955.
ModBase	Search...
Genome annotation databases
GeneID	1021674.
GenomeReviews	Gene locus BL0724 in contig AE014295_GR.
KEGG	blo:BL0724.
NMPDR	fig|206672.1.peg.672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G6C2.
OMA	IQLDFML.
Enzyme and pathway databases
BioCyc	BLON206672:BL0724-MON.
BRENDA	6.1.1.3. 39917.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BIFLO

Accession

Primary (citable) accession number: Q8G6C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)