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Q8G6B9 (RUVC_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:BL0727
OrganismBifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 194194Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183076

Sites

Metal binding71Magnesium By similarity
Metal binding681Magnesium By similarity
Metal binding1411Magnesium By similarity
Metal binding1441Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G6B9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 806A0978709D968E

FASTA19420,939
        10         20         30         40         50         60 
MIILGVDPGL TRCGVGVIEA GEHRRLSFIH VDVVRSSPDI TQDLRLLAIY NGLSEKMDRF 

        70         80         90        100        110        120 
APDAVSIERV FAQSNRNTVL GTAQAAGLAM LAAAQRNIPV ALHTPTEAKL AITGNGQAQK 

       130        140        150        160        170        180 
IQVERMVTRI LNLTKMPTPA DAADALALAI CHALRPAGAL QGGEREQHLT AAQRQWAEAA 

       190 
QNSTRRRINH DRGM 

« Hide

References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014295 Genomic DNA. Translation: AAN24544.1.
RefSeqNP_695908.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G6B9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING206672.BL0727.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN24544; AAN24544; BL0727.
GeneID1021745.
KEGGblo:BL0727.
PATRIC21117901. VBIBifLon107831_0424.

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012182.
KOK01159.
OMACHAWRGG.
OrthoDBEOG6RG044.
PhylomeDBQ8G6B9.

Enzyme and pathway databases

BioCycBLON206672:GI1E-394-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_BIFLO
AccessionPrimary (citable) accession number: Q8G6B9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families