Q8G651 (METE_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BL0798

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098614
Sites
	Metal binding	652	1	Zinc By similarity
	Metal binding	654	1	Zinc By similarity
	Metal binding	737	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G651 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 839AC629629F9D26	FASTA	767	85,359
	10 20 30 40 50 60 MSTLTSVSGF PRIGQNRELK KIIEGYWKGA NDLAAVKATA AELRAKHWKL QQAAGIDLIP 70 80 90 100 110 120 SNDFSYYDQM LDTAILLNVI PQRYARLSFD NQEDTLFAMA RGYQGDKGDV TALPMKKWFT 130 140 150 160 170 180 TNYHYLVPEV ESAAEIKLNS TKPFDEFNEA KALGIDTKPV FIGPYTFLKL ARTPEATELE 190 200 210 220 230 240 LDKGLVNAVA AVYVEVLAKF NELGAAWVQL DEPYLVLDKE PGDVELFKTL YTKILSAKGN 250 260 270 280 290 300 VKVLLNTYFG HIADVYETVN LLGFDGIGLD LNEGREENLE AVAKYGVASN TTIFAGVING 310 320 330 340 350 360 RNIWRNNYAT SLGLVDALKQ VTANVAVSTA SSLLHVPFST EGETGIPAED LKHFAFAVQK 370 380 390 400 410 420 LDELKEVAAL ADATEDEKKA SAALAANQAL FDGTRVAADP AVAERIGKLS DADYVRQPAR 430 440 450 460 470 480 EERQALQREA LGLPLLPTTT IGSFPQTKEI RAERAKLRKG EVTKEAYDEF IKAQIDAVIK 490 500 510 520 530 540 KQEEIGLDVL VHGEFERNDM VEYFGQNLNG FLFTKNAWVQ SYGTRCVKPP IVWGDVSRAN 550 560 570 580 590 600 PITVEWSAYA QSKTDHVMKG MLTGPVTILN WSWPREDITH EEQTKQLALA IRDEVLDLEA 610 620 630 640 650 660 AGIKVIQIDE AALREKLPLR KSDWHVKYLD WAVPAFRLVH SAVKPTTQIH THMCYSEFND 670 680 690 700 710 720 IIRDIDAMDA DVISFEASRG DLVVLDAIHD AHFETEAGPG VYDIHSPRIP SEKEIEDRIY 730 740 750 760 EILDKMDVKK VWINPDCGLK TRGNAETWPS LENLVAAAKA VRAKLDK

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24613.1.
RefSeq	NP_695977.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G651.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022116.
GenomeReviews	Gene locus BL0798 in contig AE014295_GR.
KEGG	blo:BL0798.
PATRIC	21118051. VBIBifLon107831_0499.
Phylogenomic databases
HOGENOM	HBG287495.
OMA	NIWANDF.
PhylomeDB	Q8G651.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BLON206672:BL0798-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BIFLO

Accession

Primary (citable) accession number: Q8G651

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program