Reviewed, UniProtKB/Swiss-Prot Q8G5X4 (AROKB_BIFLO)

Names and origin

Protein names

Recommended name:
    Bifunctional shikimate kinase/3-dehydroquinate synthase
Including the following 2 domains:
    1- Recommended name:
            Shikimate kinase
                Short name=SK
              EC=2.7.1.71
    2- Recommended name:
            3-dehydroquinate synthase
              EC=4.2.3.4

Gene names

Name:	aroB
Synonyms:	aroK/B
Ordered Locus Names:	BL0877

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. HAMAP MF_00109 ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	NAD By similarity. HAMAP MF_00109 Divalent metal cations By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. HAMAP MF_00109 Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	In the N-terminal section; belongs to the shikimate kinase family. In the C-terminal section; belongs to the dehydroquinate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Kinase Lyase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	Bifunctional shikimate kinase/3-dehydroquinate synthase HAMAP MF_00109		PRO_0000192431
Regions
	Nucleotide binding	11 – 18	8	ATP By similarity
	Region	1 – 166	166	Shikimate kinase HAMAP MF_00109
	Region	167 – 540	374	3-dehydroquinate synthase HAMAP MF_00109

Sequences

	Sequence		Length	Mass (Da)
	Q8G5X4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4619CB23F87C5B5A	FASTA	540	58,712
	10 20 30 40 50 60 MTARPRAVII GMMGAGKTRV GKEVAHMLRL PFADADVEIE REVGMKIPSY FEEYGEPAFR 70 80 90 100 110 120 EVEADLIADM LEDFDGIFSL GGGAPMTPST QHALASYIDH GGRVVYLDAD PAEAMERANR 130 140 150 160 170 180 GGGRPMLNGN ANSRWKKLFK QRDPVFREVA NVHVHTRGLT PQGAAKKVID MVSERAVHVT 190 200 210 220 230 240 GAAIEPYDVV IGEGAMNHLV DVLGPKPAKI ALIHTQPVQR HSDRARALLR QGGYEVSDIV 250 260 270 280 290 300 IPDAEPGKTI TVANGIWERL GNEGFTRSDA VVGLGGGAAT DLAGFVAATW MRGVRYVNCP 310 320 330 340 350 360 TSLLAMVDAS TGGKTGINTP QGKNLVGSFY TPAGVLADTK TLATLPNDIF IEGLGEVAKS 370 380 390 400 410 420 GFIRDPEILH ILEDHAAELR AFDGSTFLGS PLEDVVAELI ERTVKVKAYH VSSDLKEKGL 430 440 450 460 470 480 REFLNYGHTM GHAIEKLEHF RWRHGNAVAV GMVYAAELAH LIGYIDQDLV DYHRSLLASM 490 500 510 520 530 540 GLPTSWNGGS FDDVLALMHR DKKARGNELR FVVLDEIGHV VHLDNPPAEA VEEAFRRIQQ

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24690.1.
RefSeq	NP_696054.1.
3D structure databases
SMR	Q8G5X4. Positions 7-172, 176-539.
ModBase	Search...
Genome annotation databases
GeneID	1022377.
GenomeReviews	Gene locus BL0877 in contig AE014295_GR.
KEGG	blo:BL0877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG632303.
OMA	ERIREIW.
PhylomeDB	Q8G5X4.
Enzyme and pathway databases
BioCyc	BLON206672:BL0877-MONOMER.
BRENDA	2.7.1.71. 39917. 4.2.3.4. 39917.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. Fused. [Tree] MF_00110. DHQ_synthase. Fused. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. IPR016037. DHQ_synth_AroB_sub. IPR000623. Shik_kinase. [Graphical view]
PANTHER	PTHR21090:SF1. DHQ_synth_AroB. 1 hit.
Pfam	PF01761. DHQ_synthase. 1 hit. PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
TIGRFAMs	TIGR01357. aroB. 1 hit.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROKB_BIFLO

Accession

Primary (citable) accession number: Q8G5X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)