Q8G5R0 (CLPP1_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 1
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 1

Gene names

Name:	clpP1
Ordered Locus Names:	BL0944

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000179506
Sites
	Active site	136	1	By similarity
	Active site	161	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G5R0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A3EBA7E8C401F374	FASTA	233	25,829
	10 20 30 40 50 60 MASEEAKFAA RADRLAGRQG VAGFMPASAR AAQPAPTNRY IMPQFVEKTP YGMKTQDAYS 70 80 90 100 110 120 RLFEDRIIFL GVQVDDASAD DVMAQLLVLE SQDPNRDVMM YINSPGGSMT AMTAIYDTMQ 130 140 150 160 170 180 YIKPDVQTVC LGQAASAAAI LLASGTKGKR LMLPNARVLI HQPAIDQGFG KATEIEIQAK 190 200 210 220 230 EMLRMREWLE ETLAKHTGQD VEKIRRDIEV DTFLTAPEAK EYGIVDEVLE HRQ

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24756.1.
RefSeq	NP_696120.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G5R0.
ModBase	Search...
Protein family/group databases
MEROPS	S14.009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022444.
GenomeReviews	Gene locus BL0944 in contig AE014295_GR.
KEGG	blo:BL0944.
PATRIC	21118351. VBIBifLon107831_0646.
Phylogenomic databases
HOGENOM	HBG558421.
OMA	QDPYTKL.
ProtClustDB	PRK00277.
Enzyme and pathway databases
BioCyc	BLON206672:BL0944-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_BIFLO

Accession

Primary (citable) accession number: Q8G5R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program