Reviewed, UniProtKB/Swiss-Prot Q8G5Q9 (CLPP2_BIFLO)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit 2
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp 2

Gene names

Name:	clpP2
Ordered Locus Names:	BL0945

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444		PRO_0000179507
Sites
	Active site	102	1	By similarity
	Active site	127	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G5Q9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B2056BCDBD66B3DB	FASTA	207	22,724
	10 20 30 40 50 60 MSNTFATLPV MAGDDVPAGP VDPIFNRLLK DRIIWMGEEV KDDMANRICA QLLMLAAEDP 70 80 90 100 110 120 KKDIWLYINS PGGSITAGMA IYDTMQLIEP DVATVGLGMC ASMGQFLLSS GTKGKRYLTS 130 140 150 160 170 180 HARVLMHQPS GGIGGTATDV RINAELIMDM KKTMSELTAE QTGHTLEEIY RDNEYDHWFT 190 200 AQEALDYGFV DKLVTTPDTI GNNQQGE

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24757.1.
RefSeq	NP_696121.1.
3D structure databases
SMR	Q8G5Q9. Positions 23-196.
ModBase	Search...
Genome annotation databases
GeneID	1022447.
GenomeReviews	Gene locus BL0945 in contig AE014295_GR.
KEGG	blo:BL0945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	NTIPYVV.
Enzyme and pathway databases
BioCyc	BLON206672:BL0945-MONOMER.
BRENDA	3.4.21.92. 39917.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. False negative. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP2_BIFLO

Accession

Primary (citable) accession number: Q8G5Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)