Q8G5P4 (GUAA_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	BL0960

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000140097
Regions
	Domain	20 – 210	191	Glutamine amidotransferase type-1
	Domain	211 – 409	199	GMPS ATP-PPase
	Nucleotide binding	238 – 244	7	ATP By similarity
Sites
	Active site	97	1	Nucleophile By similarity
	Active site	184	1	By similarity
	Active site	186	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G5P4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 252A91136E3EE8A0	FASTA	535	57,866
	10 20 30 40 50 60 MFSPRKIPMN VRGISMANGP VLVVDFGAQY AQLIARRVRE AGVYSELVPH SMPVDEILAK 70 80 90 100 110 120 DPKAIILSGG PASVFEPGAP TIDTKVFESG VPVLGICYGF QVMAYELGGK VDKAALGEYG 130 140 150 160 170 180 KTSATIDDAA GILADSPAEQ TTWMSHGVAV EQAPAGFEVL AHTEGAPVAA MADESRKLYG 190 200 210 220 230 240 VQWHPEVKHS PLGQKLIENF LHRCAALPND WDASSIIEDQ VKKIREQVGD AEVICGLSGG 250 260 270 280 290 300 VDSAVAAALV HKAIGDQLTC VFVDHGLLRK GEVEQVKHDF VAATGIRLIT VDAADDFLDA 310 320 330 340 350 360 LAGVSEPERK RKIIGEKFIR TFEKAQRQVL EEAGARGKEV KFLVQGTLYP DVVESGGGDG 370 380 390 400 410 420 AANIKSHHNV GGLPKDIKFQ LIEPLRTLFK DEVRAIGTEL GLPDEIVWRQ PFPGPGLGIR 430 440 450 460 470 480 IIGEITKERL DLLREADAIA REELSKAGLD RDIWQCPVVL LADVHSVGVQ GDERTYGSPI 490 500 510 520 530 VLRPVSSEDA MTADWSRVPY DVLATISTRI TNECRQINRV VLDCTSKPPA TIEWE

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24772.1.
RefSeq	NP_696136.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G5P4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022462.
GenomeReviews	Gene locus BL0960 in contig AE014295_GR.
KEGG	blo:BL0960.
NMPDR	fig|206672.1.peg.903.
PATRIC	21118387. VBIBifLon107831_0664.
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
PhylomeDB	Q8G5P4.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	BLON206672:BL0960-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_BIFLO

Accession

Primary (citable) accession number: Q8G5P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program