Q8G5P2 (KPRS_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	BL0963

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAN24774.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141111
Regions
	Region	227 – 240	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	138	1	Magnesium Potential
	Metal binding	140	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential
	Metal binding	153	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G5P2 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 93A682F022A8A300	FASTA	340	36,863
	10 20 30 40 50 60 MVSAILEGKP DKNLILVTGR IHPKLAEDVA EQLGIDVLET TAYDFANGEM YVRYTESVRG 70 80 90 100 110 120 ADVFVLQSHY KPINKAIMEQ LIMIDALKRA SARSITAVCP LLGYSRQDKK HRGREPISCR 130 140 150 160 170 180 LVFDLLKTAG ADRIMSVDLH AAQSQGFFDG PVDHLVAMPV LVDYIRDRFQ GHLDNVAVVS 190 200 210 220 230 240 PDAGRIRVAE QWAQRLGGGP LAFVHKTRDI TRPNQAVANR VVGDVAGKDC VLVDDLIDTA 250 260 270 280 290 300 GTIAGACHVL QDAGAKSVTV VATHGVLSGP AVERLKNCGA REVVLTDTVP IPEEKRWDGL 310 320 330 340 TVLSIAPLLA SAIRAVFEDG SVAELFDTYP EHHGQGFLFA

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24774.1. Different initiation.
RefSeq	NP_696138.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G5P2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022464.
GenomeReviews	Gene locus BL0963 in contig AE014295_GR.
KEGG	blo:BL0963.
PATRIC	21118393. VBIBifLon107831_0667.
Phylogenomic databases
HOGENOM	HBG519284.
OMA	TDTIPLT.
PhylomeDB	Q8G5P2.
ProtClustDB	PRK03092.
Enzyme and pathway databases
BioCyc	BLON206672:BL0963-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BIFLO

Accession

Primary (citable) accession number: Q8G5P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program