Reviewed, UniProtKB/Swiss-Prot Q8G5L0 (GLGB_BIFLO)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	BL0999

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 737	737	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188684
Sites
	Active site	313	1	By similarity
	Active site	348	1	By similarity
	Active site	353	1	By similarity
	Active site	416	1	By similarity
	Active site	418	1	By similarity
	Active site	471	1	By similarity
	Active site	538	1	By similarity
	Active site	539	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G5L0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 035A1580871A21F9	FASTA	737	83,051
	10 20 30 40 50 60 MPVAQGDLDA TSNAEFYNPH GVLGGHLGIG KHADTATIRV LRPLAKSVTI LTQDGEYPMT 70 80 90 100 110 120 HEYNGVFVVT VPASGTKKQP TIPDYRVRTE WESGAVLIED DPYRYMPTVG DMDTYLFGEG 130 140 150 160 170 180 RHEKLWETLG AHVLRYDDPM GGADGTPGEQ VVGTAFSVWA PNAHAVRVVG NFNAWDGRRH 190 200 210 220 230 240 AMRELGSSGV WEIFIPGIGA GETYKFQILN ANYTWEMKAD PMERQHEVPP STASIVTEST 250 260 270 280 290 300 YKWNDDAWMQ HRRTTNPHDG PVSIYEVHAG SWKQGLTYRD LAKQLVDYVK QEGFTHVEFM 310 320 330 340 350 360 PLAQHPFSGS WGYQVTGYYA VDSRLGSPDD FRYLVDQFHQ AGIGVIMDWV PAHFPKDAFA 370 380 390 400 410 420 LGRFDGTPLY EDPDPLRGEH PEWGTYVFNF GRREVRNFLV ANALFWLEDL HVDALRVDAV 430 440 450 460 470 480 SSMLYLDYSR EPGHWRPNIY GGRENLEAID FLKEATATAY KNNPGVMVIA EESTAWPGIT 490 500 510 520 530 540 APTSAGGIGF GMKWNMGWMH DTLEYLHEEP INRKWHHNEI TFSMVYAYSE HYVLPISHDE 550 560 570 580 590 600 VVYGKGSLYG KMPGDDWQKL AGVRSMFAYQ WAHPGKKLSF MGNELAQWGE WDHDASIDWD 610 620 630 640 650 660 CLNWQEHRQV QTMVADLNAF YKAHPALWSQ DFDPAGFQWL TSDDADHNTL SFLRIGTKGE 670 680 690 700 710 720 TLAVVVNFSG EAWSDYQVAL PTGGKWTEVF TTDDAKYGGS DIHNGTFEAV EGEYHSRPFS 730 AKITVPALGV VFLKPED

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24807.1.
RefSeq	NP_696171.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Genome annotation databases
GeneID	1022518.
GenomeReviews	Gene locus BL0999 in contig AE014295_GR.
KEGG	blo:BL0999.
NMPDR	fig|206672.1.peg.938.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G5L0.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	BLON206672:BL0999-MON.
BRENDA	2.4.1.18. 39917.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BIFLO

Accession

Primary (citable) accession number: Q8G5L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)