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Q8G5L0 (GLGB_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:BL0999
OrganismBifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7377371,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188684

Sites

Active site4181Nucleophile By similarity
Active site4711Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G5L0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 035A1580871A21F9

FASTA73783,051
        10         20         30         40         50         60 
MPVAQGDLDA TSNAEFYNPH GVLGGHLGIG KHADTATIRV LRPLAKSVTI LTQDGEYPMT 

        70         80         90        100        110        120 
HEYNGVFVVT VPASGTKKQP TIPDYRVRTE WESGAVLIED DPYRYMPTVG DMDTYLFGEG 

       130        140        150        160        170        180 
RHEKLWETLG AHVLRYDDPM GGADGTPGEQ VVGTAFSVWA PNAHAVRVVG NFNAWDGRRH 

       190        200        210        220        230        240 
AMRELGSSGV WEIFIPGIGA GETYKFQILN ANYTWEMKAD PMERQHEVPP STASIVTEST 

       250        260        270        280        290        300 
YKWNDDAWMQ HRRTTNPHDG PVSIYEVHAG SWKQGLTYRD LAKQLVDYVK QEGFTHVEFM 

       310        320        330        340        350        360 
PLAQHPFSGS WGYQVTGYYA VDSRLGSPDD FRYLVDQFHQ AGIGVIMDWV PAHFPKDAFA 

       370        380        390        400        410        420 
LGRFDGTPLY EDPDPLRGEH PEWGTYVFNF GRREVRNFLV ANALFWLEDL HVDALRVDAV 

       430        440        450        460        470        480 
SSMLYLDYSR EPGHWRPNIY GGRENLEAID FLKEATATAY KNNPGVMVIA EESTAWPGIT 

       490        500        510        520        530        540 
APTSAGGIGF GMKWNMGWMH DTLEYLHEEP INRKWHHNEI TFSMVYAYSE HYVLPISHDE 

       550        560        570        580        590        600 
VVYGKGSLYG KMPGDDWQKL AGVRSMFAYQ WAHPGKKLSF MGNELAQWGE WDHDASIDWD 

       610        620        630        640        650        660 
CLNWQEHRQV QTMVADLNAF YKAHPALWSQ DFDPAGFQWL TSDDADHNTL SFLRIGTKGE 

       670        680        690        700        710        720 
TLAVVVNFSG EAWSDYQVAL PTGGKWTEVF TTDDAKYGGS DIHNGTFEAV EGEYHSRPFS 

       730 
AKITVPALGV VFLKPED 

« Hide

References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014295 Genomic DNA. Translation: AAN24807.1.
RefSeqNP_696171.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G5L0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING206672.BL0999.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN24807; AAN24807; BL0999.
GeneID1022518.
KEGGblo:BL0999.
PATRIC21118473. VBIBifLon107831_0702.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.
PhylomeDBQ8G5L0.

Enzyme and pathway databases

BioCycBLON206672:GI1E-666-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BIFLO
AccessionPrimary (citable) accession number: Q8G5L0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries