Reviewed, UniProtKB/Swiss-Prot Q8G5H9 (PROA_BIFLO)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BL1034

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189700

Sequences

	Sequence		Length	Mass (Da)
	Q8G5H9-1 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: 66243B4679DABEB0	FASTA	431	45,571
	10 20 30 40 50 60 MSDELSPEVF DAVCRQADQA ARAQERLAQA NTEAKNELLL AIADALDEHA ADIEAANALD 70 80 90 100 110 120 MLESKENGMD AGKLDRLLFD VPRVAAAAQG VRHVATLPDP VGEIVRGYNL PNGLRLTQTR 130 140 150 160 170 180 VPMGVIGMIY EARPNVTVDV ASLCLKSGNA ALLRGGHAAE RTNAATLGVI APVLEAHGFE 190 200 210 220 230 240 SALVQSVDQY GRAGATAMME ARGHIDVLVP RGGAGLIQAV VRNSKVPVIE TGAGNVHIYI 250 260 270 280 290 300 DKSGDLAKAI PIIINAKTQR VGVCNAAEKL LVHKDVAAEF LPQIAAALAE ANVVLQTDTT 310 320 330 340 350 360 SYGIISGAAI EGLDLNHAAE EDWDTEYLAL KMGIKVVSDL DAAIDHINTH STGHTESIIA 370 380 390 400 410 420 EDYAAIEEFT KRIDSAVVMV NASTRFTDGG VFGFGAELGI STQKMHARGP MGLREMTTTK 430 WIGYGTGQVR A

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24839.1. Different initiation.
RefSeq	NP_696203.1.
3D structure databases
SMR	Q8G5H9. Positions 12-431.
ModBase	Search...
Genome annotation databases
GeneID	1022561.
GenomeReviews	Gene locus BL1034 in contig AE014295_GR.
KEGG	blo:BL1034.
NMPDR	fig|206672.1.peg.970.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	Q8G5H9.
Enzyme and pathway databases
BioCyc	BLON206672:BL1034-MONOMER.
BRENDA	1.2.1.41. 39917.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BIFLO

Accession

Primary (citable) accession number: Q8G5H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)