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Protein

NAD kinase

Gene

nadK

Organism
Bifidobacterium longum (strain NCC 2705)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei71 – 711NADUniRule annotation
Binding sitei152 – 1521NADUniRule annotation
Binding sitei171 – 1711NADUniRule annotation
Binding sitei206 – 2061NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672NADUniRule annotation
Nucleotide bindingi141 – 1422NADUniRule annotation
Nucleotide bindingi182 – 1876NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLON206672:GI1E-710-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:BL1044
OrganismiBifidobacterium longum (strain NCC 2705)
Taxonomic identifieri206672 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000000439: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342NAD kinasePRO_0000120601Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi206672.BL1044.

Structurei

3D structure databases

ProteinModelPortaliQ8G5G8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227223.
KOiK00858.
OMAiGVLWCDG.
OrthoDBiEOG6PZXDR.
PhylomeDBiQ8G5G8.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G5G8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKRNAVVVT HTRLRQTGTV VAEAVSQLRV AGFEVAIIDN TEAPDFGVQP
60 70 80 90 100
PCVSDDTEIV VVLGGDGTIL RAAELVHCTQ VPILGVNMGH VGFLAEFESF
110 120 130 140 150
QIDEAIRRVS THDYSIDERM IAHVDVWLPG ATKPIEDWAL NDITLERADR
160 170 180 190 200
GKMVELSIRV DDVEMNSFGA DGVIVSTPTG STAYAFSAGG PVMWPNVKAL
210 220 230 240 250
QLIPLAAHAL FARPLIIGSG STFTIDILDD SMSEGWICCD GRRQRALPQG
260 270 280 290 300
TRVMVRESRD TLRLARLSGV PFTNRLVSKF DLPVVGWREH ARNEASSQSL
310 320 330 340
HHGHTFPAAA YAAGVAVAVA GDAGVAGTDP DKPGERDGKA GA
Length:342
Mass (Da):36,613
Last modified:March 1, 2003 - v1
Checksum:i3C215EC0045DB211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014295 Genomic DNA. Translation: AAN24850.1.
RefSeqiNP_696214.1. NC_004307.2.

Genome annotation databases

EnsemblBacteriaiAAN24850; AAN24850; BL1044.
GeneIDi1022572.
KEGGiblo:BL1044.
PATRICi21118567. VBIBifLon107831_0748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014295 Genomic DNA. Translation: AAN24850.1.
RefSeqiNP_696214.1. NC_004307.2.

3D structure databases

ProteinModelPortaliQ8G5G8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi206672.BL1044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN24850; AAN24850; BL1044.
GeneIDi1022572.
KEGGiblo:BL1044.
PATRICi21118567. VBIBifLon107831_0748.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227223.
KOiK00858.
OMAiGVLWCDG.
OrthoDBiEOG6PZXDR.
PhylomeDBiQ8G5G8.

Enzyme and pathway databases

BioCyciBLON206672:GI1E-710-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
    Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
    Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCC 2705.

Entry informationi

Entry nameiNADK_BIFLO
AccessioniPrimary (citable) accession number: Q8G5G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.