Reviewed, UniProtKB/Swiss-Prot Q8G5F2 (ASSY_BIFLO)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	BL1058

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm Probable HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148573
Regions
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Binding site	89	1	Citrulline By similarity
	Binding site	119	1	ATP; via amide nitrogen By similarity
	Binding site	121	1	Aspartate By similarity
	Binding site	125	1	Aspartate By similarity
	Binding site	125	1	Citrulline By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	129	1	Citrulline By similarity
	Binding site	177	1	Citrulline By similarity
	Binding site	261	1	Citrulline By similarity
	Binding site	273	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G5F2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 488BD98E79E6EB17	FASTA	412	45,506
	10 20 30 40 50 60 MADNKRIVLA YSGGLDTSVA ISYLKERTGK DVVAVSLDVG QGGESLETIK QRALACGAVE 70 80 90 100 110 120 SYVVDARDEF ANEYCMKALK ANAMYEGVYP LVSAISRPLI SKHLVRAAHQ FGADTISHGC 130 140 150 160 170 180 TGKGNDQVRF EVSIASIDPT LKAISPIRDL SLTRDVEIAF AKEHKLPITQ TEKSPYSIDQ 190 200 210 220 230 240 NVWGRAIETG FLEDPWNGPT KDCYSYTDDP AFPPVEDEVV IEFKEGVPVK IDGRDVTPLQ 250 260 270 280 290 300 AIEEMNRRAG AQGVGRIDLI EDRLVGIKSR ELYEAPGAVA LITAHQELEN CCLEREQHRI 310 320 330 340 350 360 KRDIDKRWGE LVYDAQWFSP ATQSLNAFIE DTQKYVSGEI RMVLHGGRAV VTGRRSDSSL 370 380 390 400 410 YDYKLATYDS GDTFDQKSSN GFIDIYGLPS RVAAARDVKF GNGIEVPKNT VE

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24866.1.
RefSeq	NP_696230.1.
3D structure databases
SMR	Q8G5F2. Positions 6-396.
ModBase	Search...
Genome annotation databases
GeneID	1022588.
GenomeReviews	Gene locus BL1058 in contig AE014295_GR.
KEGG	blo:BL1058.
NMPDR	fig|206672.1.peg.997.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335267.
OMA	IAPVREW.
PhylomeDB	Q8G5F2.
Enzyme and pathway databases
BioCyc	BLON206672:BL1058-MONOMER.
BRENDA	6.3.4.5. 39917.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_BIFLO

Accession

Primary (citable) accession number: Q8G5F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)