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Q8G527 (DAPA_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:BL1193
OrganismBifidobacterium longum (strain NCC 2705)
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000103085

Regions

Region60 – 612Pyruvate binding By similarity

Sites

Active site1711Schiff-base intermediate with substrate By similarity
Binding site1161Pyruvate By similarity
Site1431Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G527 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 75D9FA988C9635C2

FASTA30131,850
        10         20         30         40         50         60 
MSEHDMHLLD SAPFGRILPA MVTPMKSDGS VDFAAAQKLA KYLVADGADG LVVNGTTGES 

        70         80         90        100        110        120 
PVTHMDEKVE LVRAVKEVVD VPVISGAGSN DTAHTVRMVE QTQEAGADAV LVVMPYYSRP 

       130        140        150        160        170        180 
SQDGIVGHYK AVDESAEKPI IVYDVPGRTG LKVKVGTYDR LAELEHVKAV KDATGDLAAA 

       190        200        210        220        230        240 
VEKQQRTGLA WYSGDDGLFL PFLSIGAVGI ISVIAHVASN PMQQLVQAFD RGDITTARRL 

       250        260        270        280        290        300 
ANQLAPLVHA LNGDGYQAVM AKAALKVKGV IPSTTMRLPN IGPDATQLDK AEEGMRAAGL 


L

« Hide

References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014295 Genomic DNA. Translation: AAN24998.1.
RefSeqNP_696362.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G527.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1022893.
GenomeReviewsGene locus BL1193 in contig AE014295_GR.
KEGGblo:BL1193.
PATRIC21118887. VBIBifLon107831_0906.

Phylogenomic databases

HOGENOMHBG358848.
OMAHGCISVV.
PhylomeDBQ8G527.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycBLON206672:BL1193-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_BIFLO
AccessionPrimary (citable) accession number: Q8G527
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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