Reviewed, UniProtKB/Swiss-Prot Q8G527 (DAPA_BIFLO)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BL1193

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103085
Regions
	Region	60 – 61	2	Pyruvate binding By similarity
Sites
	Active site	171	1	Schiff-base intermediate with substrate By similarity
	Binding site	116	1	Pyruvate By similarity
	Site	143	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G527-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 75D9FA988C9635C2	FASTA	301	31,850
	10 20 30 40 50 60 MSEHDMHLLD SAPFGRILPA MVTPMKSDGS VDFAAAQKLA KYLVADGADG LVVNGTTGES 70 80 90 100 110 120 PVTHMDEKVE LVRAVKEVVD VPVISGAGSN DTAHTVRMVE QTQEAGADAV LVVMPYYSRP 130 140 150 160 170 180 SQDGIVGHYK AVDESAEKPI IVYDVPGRTG LKVKVGTYDR LAELEHVKAV KDATGDLAAA 190 200 210 220 230 240 VEKQQRTGLA WYSGDDGLFL PFLSIGAVGI ISVIAHVASN PMQQLVQAFD RGDITTARRL 250 260 270 280 290 300 ANQLAPLVHA LNGDGYQAVM AKAALKVKGV IPSTTMRLPN IGPDATQLDK AEEGMRAAGL L

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24998.1.
RefSeq	NP_696362.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Genome annotation databases
GeneID	1022893.
GenomeReviews	Gene locus BL1193 in contig AE014295_GR.
KEGG	blo:BL1193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G527.
OMA	VAPRLMH.
Enzyme and pathway databases
BioCyc	BLON206672:BL1193-MON.
BRENDA	4.2.1.52. 39917.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BIFLO

Accession

Primary (citable) accession number: Q8G527

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)