Reviewed, UniProtKB/Swiss-Prot Q8G4T9 (PROB_BIFLO)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BL1285

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109646
Regions
	Domain	285 – 363	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q8G4T9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2196B4E8563223FA	FASTA	377	39,188
	10 20 30 40 50 60 MSTPSQAEVR RMIAAAGTIV VKVGSSSLTQ PSGHLDPDKL DALAAALAQV RLMGGRVVLV 70 80 90 100 110 120 SSGAIAAGFG PLGFDSRPVD VATQQATAAV GQGLLMARYE TAFGRFGIRV GQILITAEDT 130 140 150 160 170 180 IRATQYRNVE RTLDRLLDLG VVPIINENDS LASNEIRFGD NDRLSALVAN LVRAEALVLL 190 200 210 220 230 240 TDVDALYTAP PSQPGSRRVE YVPNVIDALG DIQVSGSGSK VGTGGMVTKL EAARVAAVSG 250 260 270 280 290 300 IPTVLTCASN AGPAMMGDPV GTVFAPVKAR GSSRRLWIGF AADPRGTIVV DAGAGQAIRG 310 320 330 340 350 360 GRASLLAAGA LEVHGDFSAG DPVWIDAESG EHLARGLAGF DSEEIPQMLG RNTAQLKRFL 370 GPQYAHPLVH RDNLVLV

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN25086.1.
RefSeq	NP_696450.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1023196.
GenomeReviews	Gene locus BL1285 in contig AE014295_GR.
KEGG	blo:BL1285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G4T9.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	BLON206672:BL1285-MON.
BRENDA	2.7.2.11. 39917.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BIFLO

Accession

Primary (citable) accession number: Q8G4T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)