Q8G4Q6 (MURD_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	BL1321

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108974
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G4Q6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 48C3E5D791A320B8	FASTA	481	51,054
	10 20 30 40 50 60 MQVADKTVVI AGLGVSGTSL AEVLRERGTH VIGVDERKPE ADLHSFDDVD WDHVDYVMSS 70 80 90 100 110 120 PVFNPRTPFV LEAQRRGIPV MSEVEFAWQL RVNNERTGTP APWIGITGTN GKTSTTEMTS 130 140 150 160 170 180 EMLTACGLDA PTAGNIASGD MSMSLSRCAT NPQHDVLCVE LSSFQLHFTD SLALDCAAIT 190 200 210 220 230 240 NIADDHLDWH GGRENYAADK SKVFHNAKRA IVYNAQDAKV SELAAEAQTA EGCRKVGFTL 250 260 270 280 290 300 EAPQAGQIGI EDGWIVDRSG VAGGAVGESV RLAAITDFTH LAEPDGSLYP HLVADVLTAL 310 320 330 340 350 360 ALVLGLGADR DTALKALTSF KPGGHRIETV AEAAVEGGSV RFVDDSKATN GHAARASLSS 370 380 390 400 410 420 FPAKSVIWIA GGLAKGSRFE DLVKDQAHTI KAAVIIGKDQ QPMIEAFASQ APDIPVTIID 430 440 450 460 470 480 PEDNDTVMDR AVEACGTYAA AGDIVLMAPA CASMDQFKSY ADRGNRFAAA AKTWSEVHGL H

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25121.1.
RefSeq	NP_696485.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G4Q6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022800.
GenomeReviews	Gene locus BL1321 in contig AE014295_GR.
KEGG	blo:BL1321.
NMPDR	fig|206672.1.peg.1252.
PATRIC	21117379. VBIBifLon107831_0172.
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	Q8G4Q6.
ProtClustDB	CLSK573012.
Enzyme and pathway databases
BioCyc	BLON206672:BL1321-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BIFLO

Accession

Primary (citable) accession number: Q8G4Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program