Q8G4Q4 (MURC_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BL1324

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182059
Regions
	Nucleotide binding	132 – 138	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G4Q4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D41AC19B2CA2C15D	FASTA	512	53,398
	10 20 30 40 50 60 MSQDQPIVLD PTYASFDAAA RPADLGATHF IGIGGAGMSV LAEMLHAEGV AVDGSDRAHS 70 80 90 100 110 120 AKTDRLETLG ITVEFGQRAE NVAQAETVVY SSAIKPDNPE IVAAHAAGKR IVHRSDILAL 130 140 150 160 170 180 LMNGKRAVTV AGAHGKTTTS SMLSHILVNA GADPSYAIGG FIQGPDGTTL DGGHAGKGDI 190 200 210 220 230 240 LVAEADESDG SFAKYHPTIA IITNCEADHL DHYGDEAHYR AAFVAHAGRA TGHVIISIDD 250 260 270 280 290 300 PDGLAVLEAL PADVKSHTVA YGTTARESLP DLGGAAYVWI ASESETAGSG VEQLTLHLPA 310 320 330 340 350 360 AVTAGEPVSQ SVALKVPGVH NARNAAAAIS AAVLLGVSPA DAAKAAGTFL GAARRFQVRG 370 380 390 400 410 420 TVKQVTVVDD YAHHPTEIAA LLDAARRRYP DSTIRVIFQP HLFSRTKFFA HQFAKSLAKA 430 440 450 460 470 480 DDVIITGIFP AREKQADFPD ISPSTIVDAA AGLKDASAGT WIQPVEDMCL AAKMMAMRAH 490 500 510 HGDVIFTVGA GDITDMDQVL LTALEAHRES CE

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25124.1.
RefSeq	NP_696488.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G4Q4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1023159.
GenomeReviews	Gene locus BL1324 in contig AE014295_GR.
KEGG	blo:BL1324.
NMPDR	fig|206672.1.peg.1255.
PATRIC	21117385. VBIBifLon107831_0175.
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YIDDYAH.
PhylomeDB	Q8G4Q4.
ProtClustDB	CLSK573014.
Enzyme and pathway databases
BioCyc	BLON206672:BL1324-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BIFLO

Accession

Primary (citable) accession number: Q8G4Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program