Q8G4G1 (PKNA2_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase pknA2
EC=2.7.11.1

Gene names

Name:	pknA2
Ordered Locus Names:	BL1425

Organism

Bifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium longum

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 3 PASTA domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW penicillin binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	Probable serine/threonine-protein kinase pknA2		PRO_0000171184
Regions
	Domain	14 – 274	261	Protein kinase
	Domain	466 – 539	74	PASTA 1
	Domain	545 – 614	70	PASTA 2
	Domain	615 – 681	67	PASTA 3
	Nucleotide binding	20 – 28	9	ATP By similarity
Sites
	Active site	140	1	Proton acceptor By similarity
	Binding site	43	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G4G1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D7916F1B880268D1	FASTA	757	80,088
	10 20 30 40 50 60 MSENEPAQMI EGRYRIVRNI AEGGMATVYE AIDERLGRTV AIKVMHTQLA KGPHREQFVE 70 80 90 100 110 120 RFRREANSAA SIANPHIVQV YDTGEFNGLD FLVMEYVHGV NLRHEMNAQG TFSVRETLRV 130 140 150 160 170 180 VAETLDGLAS AHRAGVVHRD IKPENILIND RGHVQITDFG LAKAASQATL SSTGMLLGTA 190 200 210 220 230 240 AYLAPEMIEN NQATAQGDLY SVGIMAWEML TGKVPFDSDN PVTLVFKHVH EDVPSVATVC 250 260 270 280 290 300 QGIDPSVAAF IAHLTARQVD ARPTDGAAAA EELSQLAAKL PLEAWQYRLH AEPIGGDHTD 310 320 330 340 350 360 ATAAALVGNI AEQAPLTGVP AGAATFKPSV PAFLADDVAS NTVDTGGAAD VNPPAPPVAP 370 380 390 400 410 420 TTALDSSTPA DASAPHKTQI MAQSGSETQV LPQAGDAFTR ALAFSDEPDV ASNGTGPKKQ 430 440 450 460 470 480 RSKKPLIIVL VIVLVLAAIG GTAGWWWFAG PGSYWSVPKP DDVTCDANAS TECSLAGADW 490 500 510 520 530 540 ATYESTLKAL GIPYKTHKEY SDDVAEGKII SSSVNKTKAV VNSRISKRAN QELTVVVSKG 550 560 570 580 590 600 VRMTTIPKDI LDANSANGKD PLNALKKAGF DNVKHDESKD EYSMDTPQGV ALTISPDPGT 610 620 630 640 650 660 TAKHNDEVTV TLSKGPMPVT MPNIVGKTQD EMQAALGELK LTANVTEQYD DKVEAGQVIS 670 680 690 700 710 720 ASQEAGAQLK WGDSVDVVIS KGPEMATIPS GLVGKQESAV TKTLEGLGFE VKTDKVLGGL 730 740 750 FGTVRTVKSG DTDLSNGGKI RLRDANGNPT VITLTIV

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25222.1.
RefSeq	NP_696586.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G4G1.
ModBase	Search...
Protein-protein interaction databases
STRING	206672.BL1425.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN25222; AAN25222; BL1425.
GeneID	1021578.
KEGG	blo:BL1425.
PATRIC	21117603. VBIBifLon107831_0283.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000037187.
KO	K00870.
OMA	DFLVMEY.
ProtClustDB	CLSK573069.
Enzyme and pathway databases
BioCyc	BLON206672:GI1E-259-MONOMER.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR005543. PASTA. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF03793. PASTA. 3 hits. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00740. PASTA. 3 hits. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51178. PASTA. 3 hits. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNA2_BIFLO

Accession

Primary (citable) accession number: Q8G4G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program