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Q8G4D8 (SYL_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BL1450
OrganismBifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length987 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 987987Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151978

Regions

Motif69 – 8012"HIGH" region HAMAP-Rule MF_00049
Motif760 – 7645"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7631ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G4D8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: A5EADD58993F6EA8

FASTA987110,295
        10         20         30         40         50         60 
MSDNEKSTQT EEPNFRYNAA LAQDIENKWQ KIWDEQGTFW AANVNGDLKD GKGRNAEGRT 

        70         80         90        100        110        120 
AYFAMDMFPY PSGKGLHVGH PLGYLASDVV SRYHRMKGEN VLHAMGYDAF GLPAEQYAVQ 

       130        140        150        160        170        180 
TGQHPRVTTE ANIANMSRQL HRMGLSFDNR RTFATIDPGY VRWTQWIFSR IYDSWYDEDA 

       190        200        210        220        230        240 
TNPSGSKGSA RPIAELVAKF ESGEKAIPGH ESDGKQWSDL TDAEQQDILN DFRLAYISKS 

       250        260        270        280        290        300 
PVNWCPGLGT VLANEEVTAE GKSERGNFPV FQRELRQWSM RITKYGHRLI ADLDGINWPE 

       310        320        330        340        350        360 
KVKLMQRNWI GESHGASVHF IVATADGDKD MEIYTTRPDT LFGTTFAVVS PEHHLLENVP 

       370        380        390        400        410        420 
AEWPADVPED WKGGYANPVE AVKAYRLAAE AKTAKDRVNE AGEKTGLFTG LYATNPITGA 

       430        440        450        460        470        480 
KLPLFTADYV LMDYGTGAIM AVPGGDQRDY DFAVKFGLPV IYTVTPLPDS GDDLANYEGK 

       490        500        510        520        530        540 
APFVSHDGIV INSSVEATEA KGDALSLNGL RVDDAIAKVN AWLESAGVGK GTVSYRLRDW 

       550        560        570        580        590        600 
LFSRQRYWGE PFPIVYGEDG TPHLLPDSAL PINLPDVPDY EPRTFDPMDA ESNPEAPLSR 

       610        620        630        640        650        660 
NEDWVKVELD LGDGKKTYYR DTNTMPNWAG SCWYYMRYID PTDTKHMVEK DEFDYWMGPN 

       670        680        690        700        710        720 
HNKYSGDEGG VDLYIGGVEH AVLHLLYSRF WHKVLFDLGY VDSAEPFHKL FNQGMIQAYA 

       730        740        750        760        770        780 
YTDDRGQYVP ADEVVEGPAD ASGEPTFTWN GEHANREFGK MGKSLKNIVT PDYMYENYGA 

       790        800        810        820        830        840 
DTFRLYEMSM GPLDESRPWN TRNVVGGMRF LQRLWRNVVD ETTGQAHVTE DTPDEKTLKL 

       850        860        870        880        890        900 
LNNTIAEVTA EMEGMRPNTA IAKLIVLNNH LTGLKAVPRA AVEPLILMLA PIAPHICEEM 

       910        920        930        940        950        960 
WSKLGHAESL SAEPWPVADE RYVGHDTVTA VVQIKGKVRA KLEVPVDIDP ADLEKQALAA 

       970        980 
VADRLGGKEP RKVIVKAPKI VSIVPAE 

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References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014295 Genomic DNA. Translation: AAN25245.1.
RefSeqNP_696609.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G4D8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING206672.BL1450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN25245; AAN25245; BL1450.
GeneID1021602.
KEGGblo:BL1450.
PATRIC21117653. VBIBifLon107831_0308.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAYARYHRM.
OrthoDBEOG63Z74X.
PhylomeDBQ8G4D8.

Enzyme and pathway databases

BioCycBLON206672:GI1E-287-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BIFLO
AccessionPrimary (citable) accession number: Q8G4D8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries