Reviewed, UniProtKB/Swiss-Prot Q8G4D1 (GCP_BIFLO)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BL1457

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303281
Sites
	Metal binding	113	1	Zinc Potential
	Metal binding	117	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G4D1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9E3158D57867AF08	FASTA	347	36,072
	10 20 30 40 50 60 MSEPVVLGIE STCDETAAAI VRGRELLSNV VASSMEEHAR YGGVIPEIAS RAHAEAFVPC 70 80 90 100 110 120 VSKALVDANM TLADVDAIAV SAGPGLAGCL AVGVSGAKAL AWAANKPIYG INHVIGHIAV 130 140 150 160 170 180 TQLQFGPFPK DTLALIVSGG HTSLLHVEDM PRKIDVVGTT LDDAAGECFD KVARLLGFPY 190 200 210 220 230 240 PGGPHIDRHG QNGDPHAIKV PMGLTQGKAG AAHPYDFSFS GVKTAVARWV ESEQAAGHEI 250 260 270 280 290 300 PVDDVCASLA DSVATVLARK AMRGCRQYDS NTLIVGGGFS ANSQLRAKLL EFGENYGVDV 310 320 330 340 RIPQIKLCTD NGAMVAMLGV NLVEAGVAPS APDFPIDSAM PLTKVSM

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25252.1.
RefSeq	NP_696616.1.
3D structure databases
SMR	Q8G4D1. Positions 5-331.
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	1021610.
GenomeReviews	Gene locus BL1457 in contig AE014295_GR.
KEGG	blo:BL1457.
NMPDR	fig|206672.1.peg.1383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	EHSAYGG.
PhylomeDB	Q8G4D1.
Enzyme and pathway databases
BioCyc	BLON206672:BL1457-MONOMER.
BRENDA	3.4.24.57. 39917.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BIFLO

Accession

Primary (citable) accession number: Q8G4D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)