Reviewed, UniProtKB/Swiss-Prot Q8G3T9 (TYSY_BIFLO)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BL1665

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Thymidylate synthase HAMAP MF_00008		PRO_0000140937
Sites
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G3T9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E8BDC2C4B8B9DE70	FASTA	266	30,689
	10 20 30 40 50 60 MPYEDLVRKI LTEGTLKSDR TGTGTISLFG QQMRFDLSKY FPLLTTKTVF FKGLAYELLW 70 80 90 100 110 120 FLKGSSNINW LLEHNVHIWD EWADENGDLG PVYGVQWRSW PAPTPEDPNR TIDQISNVLD 130 140 150 160 170 180 LIKHHPDSRR MIVSAWNPAE VEKMALPPCH ALFQFYVADD KLSCQLYQRS CDMFLGVPFN 190 200 210 220 230 240 IASYSLLTMM MAQQAGLKPG EFVWTGGDCH VYDNHVDQFL EQLGRKPYPY PTIEIRKADS 250 260 LFDYQYEDFK VVGYQHHPTI KAPVAV

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25452.1.
RefSeq	NP_696816.1.
3D structure databases
SMR	Q8G3T9. Positions 3-266.
ModBase	Search...
Genome annotation databases
GeneID	1021894.
GenomeReviews	Gene locus BL1665 in contig AE014295_GR.
KEGG	blo:BL1665.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	Q8G3T9.
Enzyme and pathway databases
BioCyc	BLON206672:BL1665-MONOMER.
BRENDA	2.1.1.45. 39917.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BIFLO

Accession

Primary (citable) accession number: Q8G3T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)