Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q8G3T4 (TRMB_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BL1670
OrganismBifidobacterium longum (strain NCC 2705) [Reference proteome] [HAMAP]
Taxonomic identifier206672 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium ›

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171300

Regions

Region262 – 2654Substrate binding Potential

Sites

Binding site911S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1651S-adenosyl-L-methionine By similarity
Binding site1691Substrate By similarity
Binding site2011Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q8G3T4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: FDC5381468395365

FASTA28631,253
        10         20         30         40         50         60 
MTNPESTAID PVAAMGTDHT EAPAHPLHKV LSFVRRSGRL DDRLQRAWDN YAGTYLLDIA 

        70         80         90        100        110        120 
AGNLLDVREG VTLDRAFVES AWGNDNPLIV EIGTGQGENV VAAAAAHPET NFLALEVYDP 

       130        140        150        160        170        180 
GVAHTLLLAG KQGLTNIRVA QVNAPELFKV TAAGTVAEVW TFFPDPWPKK KHHKRRIVQK 

       190        200        210        220        230        240 
AMAGDIHRAL VTDGVWRIAT DIEDYALHVH EVMDGLDGWK NLGSVTVSLP LEHVGKGNAD 

       250        260        270        280 
MAADMPHADF TESERFEGRV LTNFEKKGLA AGRVIHDFTY QAVTLN

« Hide

References

[1]"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCC 2705.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014295 Genomic DNA. Translation: AAN25457.1.
RefSeqNP_696821.1. NC_004307.2.

3D structure databases

ProteinModelPortalQ8G3T4.
ModBaseSearch...

Protein-protein interaction databases

STRING206672.BL1670.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN25457; AAN25457; BL1670.
GeneID1021903.
KEGGblo:BL1670.
PATRIC21120604. VBIBifLon107831_1725.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMACEVHEPG.
ProtClustDBCLSK573176.

Enzyme and pathway databases

BioCycBLON206672:GI1E-1654-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_BIFLO
AccessionPrimary (citable) accession number: Q8G3T4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: May 29, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents