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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Bifidobacterium longum (strain NCC 2705)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 56ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:BL1680
OrganismiBifidobacterium longum (strain NCC 2705)
Taxonomic identifieri206672 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000000439 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816551 – 388tRNA(Ile)-lysidine synthaseAdd BLAST388

Interactioni

Protein-protein interaction databases

STRINGi206672.BL1680.

Structurei

3D structure databases

ProteinModelPortaliQ8G3S4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RD1. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236508.
KOiK04075.
OMAiPWCVALS.
PhylomeDBiQ8G3S4.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G3S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYSARLRKA VGAVRATLSA VELCDVQAPE FAQHGDHAVA ADAPLVLVAC
60 70 80 90 100
SGGRDSMALA AVSHIVCTSM GVRCGAVIVD HGLQAGSERV ASEAADRCRA
110 120 130 140 150
LGLGPVIMRN ATVQARGEGL EAAARQARYD ELCAAAHESG AIAVLLAHTM
160 170 180 190 200
DDQAETVLIG LLRSRGVDAL AGMPQVFTRS GATFARPLLT LTRAETTGIC
210 220 230 240 250
EDLGVEYWDD PTNGDAVDGE LPNDYPLRSR VRHDLLPAIE RFAGFNVTRH
260 270 280 290 300
FAESARLARM DKEYLDQRSD EVMGEAVAAV DRPASSAAVS TDAPRACVAG
310 320 330 340 350
DTNDSGHGIG LMIGVKRIAR EPEAIRLRVI AHALSQAGVN ASAAQIAAID
360 370 380
RLVVDWHGQG GVSLPRGYSA NRKKHVIRVC QDGAHANR
Length:388
Mass (Da):40,903
Last modified:March 1, 2003 - v1
Checksum:i52EA8150881ACFC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014295 Genomic DNA. Translation: AAN25467.1.
RefSeqiNP_696831.1. NC_004307.2.
WP_011068788.1. NC_004307.2.

Genome annotation databases

EnsemblBacteriaiAAN25467; AAN25467; BL1680.
GeneIDi1021921.
KEGGiblo:BL1680.
PATRICi21120628. VBIBifLon107831_1735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014295 Genomic DNA. Translation: AAN25467.1.
RefSeqiNP_696831.1. NC_004307.2.
WP_011068788.1. NC_004307.2.

3D structure databases

ProteinModelPortaliQ8G3S4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi206672.BL1680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN25467; AAN25467; BL1680.
GeneIDi1021921.
KEGGiblo:BL1680.
PATRICi21120628. VBIBifLon107831_1735.

Phylogenomic databases

eggNOGiENOG4107RD1. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236508.
KOiK04075.
OMAiPWCVALS.
PhylomeDBiQ8G3S4.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_BIFLO
AccessioniPrimary (citable) accession number: Q8G3S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.