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Protein

Release factor glutamine methyltransferase

Gene

prmC

Organism
Bifidobacterium longum (strain NCC 2705)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei148 – 1481S-adenosyl-L-methionineUniRule annotation
Binding sitei201 – 2011S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBLON206672:GI1E-1698-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Release factor glutamine methyltransferaseUniRule annotation (EC:2.1.1.297UniRule annotation)
Short name:
RF MTaseUniRule annotation
Alternative name(s):
N5-glutamine methyltransferase PrmCUniRule annotation
Protein-(glutamine-N5) MTase PrmCUniRule annotation
Protein-glutamine N-methyltransferase PrmCUniRule annotation
Gene namesi
Name:prmCUniRule annotation
Ordered Locus Names:BL1713
OrganismiBifidobacterium longum (strain NCC 2705)
Taxonomic identifieri206672 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000000439 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Release factor glutamine methyltransferasePRO_0000414502Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi206672.BL1713.

Structurei

3D structure databases

ProteinModelPortaliQ8G3P4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 2044Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076276.
KOiK02493.
OMAiILGAWEF.
OrthoDBiEOG68Q0SZ.
PhylomeDBiQ8G3P4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G3P4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLITDIINQA SSQLREAGIE TPEHDAKLLL AEAAGVELRD VDRALLMGEE
60 70 80 90 100
LGTTGQLAVF RAMLDRRAKR EPLQYITGHA PFRYLDLKVG PGVFIPRPET
110 120 130 140 150
ETVVQAGLDW LTKNGMIHPC VVDLCAGSGA IGLSVVSEVP GSQVWAVELS
160 170 180 190 200
PNTAEWTRRN LSETAKKYPS IASNYHLEIA DATSFATLAQ LDGTVDIVIT
210 220 230 240 250
NPPYVPQTDI PEQPEVRDWD PELALYGGSM DGTLIPERII ERACRLLKPG
260 270 280 290
GVLVMEHDVT QGDRLVAFAR ATGFAAASTG QDWTGRDRYL FAVS
Length:294
Mass (Da):32,022
Last modified:March 1, 2003 - v1
Checksum:i2CC4A7D270DAD4D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014295 Genomic DNA. Translation: AAN25497.1.
RefSeqiNP_696861.1. NC_004307.2.
WP_007055663.1. NC_004307.2.

Genome annotation databases

EnsemblBacteriaiAAN25497; AAN25497; BL1713.
GeneIDi1021962.
KEGGiblo:BL1713.
PATRICi21120694. VBIBifLon107831_1766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014295 Genomic DNA. Translation: AAN25497.1.
RefSeqiNP_696861.1. NC_004307.2.
WP_007055663.1. NC_004307.2.

3D structure databases

ProteinModelPortaliQ8G3P4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi206672.BL1713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN25497; AAN25497; BL1713.
GeneIDi1021962.
KEGGiblo:BL1713.
PATRICi21120694. VBIBifLon107831_1766.

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076276.
KOiK02493.
OMAiILGAWEF.
OrthoDBiEOG68Q0SZ.
PhylomeDBiQ8G3P4.

Enzyme and pathway databases

BioCyciBLON206672:GI1E-1698-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
    Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
    Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCC 2705.

Entry informationi

Entry nameiPRMC_BIFLO
AccessioniPrimary (citable) accession number: Q8G3P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.