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Q8G3I2

- SYI_BIFLO

UniProt

Q8G3I2 - SYI_BIFLO

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BL1777
Organism
Bifidobacterium longum (strain NCC 2705)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei652 – 6521ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBLON206672:GI1E-1762-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BL1777
OrganismiBifidobacterium longum (strain NCC 2705)
Taxonomic identifieri206672 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000000439: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11031103Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098524Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi206672.BL1777.

Structurei

3D structure databases

ProteinModelPortaliQ8G3I2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi65 – 7511"HIGH" regionUniRule annotation
Add
BLAST
Motifi649 – 6535"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.
PhylomeDBiQ8G3I2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8G3I2-1 [UniParc]FASTAAdd to Basket

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MSENVYPKAN EGGETAHVAP NPSFPDMEET VLDYWDKDDT FQKSVERNPS     50
GDHSQNEFVF FDGPPFANGL PHYGHLLTGY AKDVIPRYQT MKGRKVNRVF 100
GWDTHGLPAE LEAQKELGID SVDQIEKMGI DKFNDACRAS VLKYTNEWQN 150
YVHRQARWVD FEHGYKTLNI PYMESVMWAF KQLYDKGLAY QGYRVLPYCP 200
KDRTPLSAHE LRMDADVYQD RQDTTVSVAV KMRDEDDAYA VFWTTTPWTV 250
PTNFAIVVGG DIDYVEVRPT EGKFAGKKFY LGKDLLPHYE KELGENYEVV 300
RELKGSELEG RRYYPVFPYF AGDEAESEGH VPGPNGYTIF TADYVDTVEG 350
TGLVHQAPYG EDDMNTLNAK GIKSTDVLDD GCRFTAQCPD YEGDFVFDAN 400
LPILRNLRAG DGPLAEIPEE RRAILFQEKS YVHSYPHCWR CATPLIYKPV 450
SSWFVSVTKI KPRLLELNQQ INWIPGNVKD GQFGKWLANA RDWSISRNRF 500
WGSPIPVWVS DDPKYPRVDV YGSLEELKAD FGDYPRDKDG NINMHRPWID 550
NLVRVNPDDP TGKSHMHRIS DVLDCWFESG SMSFAQFHYP FENKEKFEQH 600
FPADYIVEYI GQTRGWFYLL HVMATALFDR PAFKNVICHG IVLGSDGQKM 650
SKHLRNYPDV NGVFDKYGSD AMRWFLMSSP ILRGGNLIVT ADGIRDTVRQ 700
VMLPVWSSYY FFTLYANAAN GGAGFDARQL RADEVAGLPE MDRYLLARTR 750
RLVLAAEKSL NEFAISDACD AVSDFIDVLT NWYIRNTRDR FWNEDASAFN 800
TLYTVLEAFM RVLAPLAPME AESVWRGLTG GESVHLAEWP FVVDEKTGAD 850
TELGRVLVDD PALVDAMEKV REVVSGTLSL RKAAKIRVRQ PLSKLTVVAG 900
NVEAVKAYDD LLKAELNIKN IEFSTLQDAA AHGLKIVHEL RVNARAAGPR 950
LGKQVQFAIK ASKSGDWHVD AASGAPVVST PSGDLALVEG EYELINRVEE 1000
ENATEAAASV SAALPTGGFV ILDTALDADL LAEGYARDVI RSVQDARKAA 1050
DLDIADRISL VLTVPAVDVA KVEQFRDLIA HETLATSFEV KEGAELGVEV 1100
VKA 1103
Length:1,103
Mass (Da):124,066
Last modified:March 1, 2003 - v1
Checksum:iB09FBEA401C18C28
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014295 Genomic DNA. Translation: AAN25560.1.
RefSeqiNP_696924.1. NC_004307.2.

Genome annotation databases

EnsemblBacteriaiAAN25560; AAN25560; BL1777.
GeneIDi1022079.
KEGGiblo:BL1777.
PATRICi21120828. VBIBifLon107831_1832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014295 Genomic DNA. Translation: AAN25560.1 .
RefSeqi NP_696924.1. NC_004307.2.

3D structure databases

ProteinModelPortali Q8G3I2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 206672.BL1777.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN25560 ; AAN25560 ; BL1777 .
GeneIDi 1022079.
KEGGi blo:BL1777.
PATRICi 21120828. VBIBifLon107831_1832.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.
PhylomeDBi Q8G3I2.

Enzyme and pathway databases

BioCyci BLON206672:GI1E-1762-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract."
    Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.
    Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCC 2705.

Entry informationi

Entry nameiSYI_BIFLO
AccessioniPrimary (citable) accession number: Q8G3I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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