Q8G3I2 (SYI_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	BL1777

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	1103 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003
Cofactor	Zinc By similarity. HAMAP MF_02003
Subunit structure	Monomer By similarity. HAMAP MF_02003
Subcellular location	Cytoplasm By similarity HAMAP MF_02003.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1103	1103	Isoleucine--tRNA ligase HAMAP MF_02003		PRO_0000098524
Regions
	Motif	65 – 75	11	"HIGH" region HAMAP MF_02003
	Motif	649 – 653	5	"KMSKS" region HAMAP MF_02003
Sites
	Binding site	652	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G3I2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B09FBEA401C18C28	FASTA	1,103	124,066
	10 20 30 40 50 60 MSENVYPKAN EGGETAHVAP NPSFPDMEET VLDYWDKDDT FQKSVERNPS GDHSQNEFVF 70 80 90 100 110 120 FDGPPFANGL PHYGHLLTGY AKDVIPRYQT MKGRKVNRVF GWDTHGLPAE LEAQKELGID 130 140 150 160 170 180 SVDQIEKMGI DKFNDACRAS VLKYTNEWQN YVHRQARWVD FEHGYKTLNI PYMESVMWAF 190 200 210 220 230 240 KQLYDKGLAY QGYRVLPYCP KDRTPLSAHE LRMDADVYQD RQDTTVSVAV KMRDEDDAYA 250 260 270 280 290 300 VFWTTTPWTV PTNFAIVVGG DIDYVEVRPT EGKFAGKKFY LGKDLLPHYE KELGENYEVV 310 320 330 340 350 360 RELKGSELEG RRYYPVFPYF AGDEAESEGH VPGPNGYTIF TADYVDTVEG TGLVHQAPYG 370 380 390 400 410 420 EDDMNTLNAK GIKSTDVLDD GCRFTAQCPD YEGDFVFDAN LPILRNLRAG DGPLAEIPEE 430 440 450 460 470 480 RRAILFQEKS YVHSYPHCWR CATPLIYKPV SSWFVSVTKI KPRLLELNQQ INWIPGNVKD 490 500 510 520 530 540 GQFGKWLANA RDWSISRNRF WGSPIPVWVS DDPKYPRVDV YGSLEELKAD FGDYPRDKDG 550 560 570 580 590 600 NINMHRPWID NLVRVNPDDP TGKSHMHRIS DVLDCWFESG SMSFAQFHYP FENKEKFEQH 610 620 630 640 650 660 FPADYIVEYI GQTRGWFYLL HVMATALFDR PAFKNVICHG IVLGSDGQKM SKHLRNYPDV 670 680 690 700 710 720 NGVFDKYGSD AMRWFLMSSP ILRGGNLIVT ADGIRDTVRQ VMLPVWSSYY FFTLYANAAN 730 740 750 760 770 780 GGAGFDARQL RADEVAGLPE MDRYLLARTR RLVLAAEKSL NEFAISDACD AVSDFIDVLT 790 800 810 820 830 840 NWYIRNTRDR FWNEDASAFN TLYTVLEAFM RVLAPLAPME AESVWRGLTG GESVHLAEWP 850 860 870 880 890 900 FVVDEKTGAD TELGRVLVDD PALVDAMEKV REVVSGTLSL RKAAKIRVRQ PLSKLTVVAG 910 920 930 940 950 960 NVEAVKAYDD LLKAELNIKN IEFSTLQDAA AHGLKIVHEL RVNARAAGPR LGKQVQFAIK 970 980 990 1000 1010 1020 ASKSGDWHVD AASGAPVVST PSGDLALVEG EYELINRVEE ENATEAAASV SAALPTGGFV 1030 1040 1050 1060 1070 1080 ILDTALDADL LAEGYARDVI RSVQDARKAA DLDIADRISL VLTVPAVDVA KVEQFRDLIA 1090 1100 HETLATSFEV KEGAELGVEV VKA

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25560.1.
RefSeq	NP_696924.1. NC_004307.2.
3D structure databases
ProteinModelPortal	Q8G3I2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022079.
GenomeReviews	Gene locus BL1777 in contig AE014295_GR.
KEGG	blo:BL1777.
NMPDR	fig|206672.1.peg.1691.
PATRIC	21120828. VBIBifLon107831_1832.
Phylogenomic databases
HOGENOM	HBG577712.
OMA	QFGKWLE.
PhylomeDB	Q8G3I2.
ProtClustDB	PRK06039.
Enzyme and pathway databases
BioCyc	BLON206672:BL1777-MONOMER.
Family and domain databases
HAMAP	MF_02003. Ile_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt. IPR023586. Ile-tRNA-synt_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01870.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. IleS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BIFLO

Accession

Primary (citable) accession number: Q8G3I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program