Q8G3H2 (ILVD_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BL1788

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103433
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G3H2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 463F654306027A8E	FASTA	620	66,558
	10 20 30 40 50 60 MTEMRSAKIM SGRVFAGARA LYRAAGVSGD DMGKKPIIAI ANSFDEFLPG HVHLNKVGRI 70 80 90 100 110 120 VSEAIREAGG IPREFNTMAV DDGIAMGHTG MLYSLPSRDI IADTVEYQCN AHCADALICI 130 140 150 160 170 180 PNCDKVVPGM LMAALRLNIP TVFVSGGPME AGTTVLADGT VKSTDLIDVM YATADDNVSD 190 200 210 220 230 240 EELLNYEKTV CPTCGSCAGM FTANSMNCLT EAIGLALPGN GTILASHSYR KDLFKRAAKQ 250 260 270 280 290 300 VVKIAHQYYD DSDDSVLPRS IATKEAFENA MTMDVAMGGS TNTVLHILAM AQSADVDFTL 310 320 330 340 350 360 DDIERISHTV PCICKASPSG KWEISDVHRA GGITGILGEL DRAGKLHTNV HSIDYPTLEA 370 380 390 400 410 420 KLADWDIMRP TCTEEAQQMY KAAPGHIISP EPWTHTTLFD SLDRDRTNGA IHDINHPEIH 430 440 450 460 470 480 EGGLAVLRGN LAPDGCVVKT AGVPPEIWKF RGPALVVDSQ EQAIEVILND TLKPGMALVI 490 500 510 520 530 540 RYEGPKGGPG MQEMLYPTSF VKGKGIGKQV AMLTDGRYSG GSSGLAIGHM APEAANKGPV 550 560 570 580 590 600 ALIKNGDIID IDIEARSVNV ELTDEQLDER RRELEAGDGY VAHRNRHVSQ ALKAYAAFAR 610 620 SADKGATRDP ELINKLSGLD

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN25571.1.
RefSeq	NP_696935.1. NC_004307.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022105.
GenomeReviews	Gene locus BL1788 in contig AE014295_GR.
KEGG	blo:BL1788.
NMPDR	fig|206672.1.peg.1702.
PATRIC	21120846. VBIBifLon107831_1841.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	MALVIRY.
PhylomeDB	Q8G3H2.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BLON206672:BL1788-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BIFLO

Accession

Primary (citable) accession number: Q8G3H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program