Q8G3B2 (KDSB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	BR0038, BS1330_I0038

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential HAMAP MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000370017

Sequences

	Sequence		Length	Mass (Da)
	Q8G3B2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8EFDB1A32037C2B6	FASTA	251	27,334
	10 20 30 40 50 60 MLQTMKTLTL IPTRLGSTRL PNKPLADICG KPMIVHVADR AAAAKLGRTV IATDSEEIFK 70 80 90 100 110 120 VVAAHGHEAI MTRGDHESGS DRIYEALAKL DPSGEIDAVV NVQGDLPTID PDTIRRALLP 130 140 150 160 170 180 LEDGPADIAT LGVEITVEEE KTNPNVVKIV GSPLAGNRRL RALYFTRATA PYGEGPLYHH 190 200 210 220 230 240 IGLYAYRRSA LERFVKLGPS PLEKREKLEQ LRALEAGMRI DVEIVKTVPL GVDTQADLDR 250 ARTFCSQAGT I

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN28995.1. CP002997 Genomic DNA. Translation: AEM17407.1.
RefSeq	NP_697080.1. NC_004310.3.
3D structure databases
HSSP	HSSP built from PDB template 1VIC based on UniProtKB P44490.
ProteinModelPortal	Q8G3B2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165695.
GenomeReviews	Gene locus BR0038 in contig AE014291_GR.
KEGG	bms:BR0038.
NMPDR	fig|204722.1.peg.37.
PATRIC	17788337. VBIBruSui107850_0042.
TIGR	BR0038.
Phylogenomic databases
HOGENOM	HBG637773.
OMA	INVQADE.
PhylomeDB	Q8G3B2.
ProtClustDB	PRK05450.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0038-MONOMER.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
KO	K00979.
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. KdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_BRUSU

Accession

Primary (citable) accession number: Q8G3B2
Secondary accession number(s): G0KAL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program