Q8G3B2 (KDSB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	BR0038, BS1330_I0038

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP-Rule MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP-Rule MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP-Rule MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP-Rule MF_00057
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP-Rule MF_00057		PRO_0000370017

Sequences

	Sequence		Length	Mass (Da)
	Q8G3B2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8EFDB1A32037C2B6	FASTA	251	27,334
	10 20 30 40 50 60 MLQTMKTLTL IPTRLGSTRL PNKPLADICG KPMIVHVADR AAAAKLGRTV IATDSEEIFK 70 80 90 100 110 120 VVAAHGHEAI MTRGDHESGS DRIYEALAKL DPSGEIDAVV NVQGDLPTID PDTIRRALLP 130 140 150 160 170 180 LEDGPADIAT LGVEITVEEE KTNPNVVKIV GSPLAGNRRL RALYFTRATA PYGEGPLYHH 190 200 210 220 230 240 IGLYAYRRSA LERFVKLGPS PLEKREKLEQ LRALEAGMRI DVEIVKTVPL GVDTQADLDR 250 ARTFCSQAGT I

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN28995.1. CP002997 Genomic DNA. Translation: AEM17407.1.
RefSeq	NP_697080.1. NC_004310.3. YP_005614899.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G3B2.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0038.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN28995; AAN28995; BR0038. AEM17407; AEM17407; BS1330_I0038.
GeneID	1165695. 12137252.
KEGG	bms:BR0038. bsi:BS1330_I0038.
PATRIC	17788337. VBIBruSui107850_0042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1212.
HOGENOM	HOG000007602.
KO	K00979.
OMA	NSGTERC.
ProtClustDB	PRK05450.
Enzyme and pathway databases
UniPathway	UPA00030. UPA00358; UER00476.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth.
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. kdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_BRUSU

Accession

Primary (citable) accession number: Q8G3B2
Secondary accession number(s): G0KAL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program