Reviewed, UniProtKB/Swiss-Prot Q8G381 (DNAE2_BRUSU)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	BR0069

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	1077 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1077	1077	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103372

Sequences

	Sequence		Length	Mass (Da)
	Q8G381-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B5B34688FA6C171C	FASTA	1,077	120,961
	10 20 30 40 50 60 MVPYFEMAAA SNFSFLCGAS HPQELVERAH ALDLSGIGIA DRNTLAGVVR AHAQWKDIRK 70 80 90 100 110 120 ESGFRLFIGC RLSFIDGTPD MVVYPRDRAA YGQLCRLLTE GKHRAAIKGE CHLEWADLLF 130 140 150 160 170 180 RARQFQIAVF PPDEDEPDFA ARLTEIAQAA PGSVWLALTM PHQGQDGRRA ERIARFAAQA 190 200 210 220 230 240 GVPLIATNDV LYHHPDRRPL QDVLTATRHH TTVFAAGRLL EKNAERHLKP PHEMVRLFRD 250 260 270 280 290 300 YPEAIAATAD FVAPITFQLD ELKYAYPDEP IPPGKTAQQH LYDLVWEGAA RHYGADIIPP 310 320 330 340 350 360 KVQGLINKEL ALIARLEYEP YFLTVYDIVT HAREKGILCQ GRGSAANSVV CFCLGITGVN 370 380 390 400 410 420 PTQVDLLFER FISAERKEPP DIDVDFEHER REEVMQYVYD RYSRDRAAIV ATVISYRSRS 430 440 450 460 470 480 AIRDVGKALG LSEDVTAALA NTVWGLSGGG IDRQHIRQAG LDPDNPIIQR AVELAITLIG 490 500 510 520 530 540 FPRHLSQHVG GFVLARDRLD ETVPIGPAAM DKRSFIEWDK DDIDEVGLMK VDVLSLGMLT 550 560 570 580 590 600 CIRKAFDLIH QHKPQLYGGE KLTLASLPRE DKAVYDMLCK GDSLGVFQVE SRAQMNMLPR 610 620 630 640 650 660 LRPQEFYDLV IEVAIVRPGP IQGDMVHPYL RRRSGQEPCT LPSPSPQHGP ANELQQILGK 670 680 690 700 710 720 TKGVPLFQEQ AMRIAMEAAK FTPEEANQLR RAMATFRKMG TIHTMEKKMI DGMVNRGYDR 730 740 750 760 770 780 TFAENCFNQI KGFGEYGFPE SHAASFAHLV YISAWLKCHH PEVFAAALLN SQPMVFYAPA 790 800 810 820 830 840 QIVRDAREHG VTVLPVDVNF SQWDNILEET PDVHLALRLG FRQIDGFSKR DTELLIADRQ 850 860 870 880 890 900 EPYRTIEDMH RRLRLDRRAF TLLADADAFG SLDIDRRAAL WAVRRLPNDE TLPLFRAAAA 910 920 930 940 950 960 SELAQEPRTK LPEMAASEHV IADYETTRLS LKGHPLQYLR EGLAAEGVST CRAVQEGADG 970 980 990 1000 1010 1020 RRMKVAGVVT VRQRPGSAKG VVFLTIEDET GIANIVIWPK IMKVFRREVM SARLIHIEGR 1030 1040 1050 1060 1070 IQRSLEGVVH LVAAKLQDRS AALIEMSGRE AQRLIAPSQM AHHPRNVRVM PNSRDFH

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29026.1.
RefSeq	NP_697111.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165726.
GenomeReviews	Gene locus BR0069 in contig AE014291_GR.
KEGG	bms:BR0069.
NMPDR	fig|204722.1.peg.68.
TIGR	BR0069.
Phylogenomic databases
HOGENOM	Q8G381.
OMA	MGFYSPD
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0069-MON.
BRENDA	2.7.7.7. 281610.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BRUSU

Accession

Primary (citable) accession number: Q8G381

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)