Q8G376 (ASSY_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate synthase
EC=6.3.4.5

Alternative name(s):
Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	BR0074, BS1330_I0074

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm By similarity HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW argininosuccinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148575
Regions
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Binding site	40	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	91	1	Citrulline By similarity
	Binding site	96	1	Citrulline By similarity
	Binding site	121	1	ATP; via amide nitrogen By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	128	1	Aspartate By similarity
	Binding site	131	1	Citrulline By similarity
	Binding site	182	1	Citrulline By similarity
	Binding site	191	1	Citrulline By similarity
	Binding site	267	1	Citrulline By similarity
	Binding site	279	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G376 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 534F12707F0E7A9D	FASTA	406	45,254
	10 20 30 40 50 60 MSKWKDVKKV VLAYSGGLDT SIILKWLQTE LGAEVVTFTA DLGQGEELEP ARKKAEMLGI 70 80 90 100 110 120 KEIFIEDVRE EFVRDFVFPM FRANAVYEGV YLLGTSIARP LISKHLIDIA KKTGADAIAH 130 140 150 160 170 180 GATGKGNDQV RFELSAYALN PDIKIIAPWR DWSFKSRTQL LEFAEQHQIP VAKDKKGEAP 190 200 210 220 230 240 FSVDANLLHS SSEGKVLEDP SQEAPEYVHM RTISPETAPD KATIIKIGFE KGDAVSINGE 250 260 270 280 290 300 RLSPATLLAK LNDYGRDNGI GRLDLVENRF VGMKSRGVYE TPGGTILLAA HRAIESITLD 310 320 330 340 350 360 RGAAHLKDEL MPRYAELIYY GFWFSPEREM LQAAIDHSQH HVEGEVTLKL YKGNVMVIGR 370 380 390 400 ESAKSLYSDK LVTFEDDQGA YDQKDAAGFI KLNALRLRTL AARDRK

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29031.1. CP002997 Genomic DNA. Translation: AEM17443.1.
RefSeq	NP_697116.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G376.
SMR	Q8G376. Positions 9-405.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165731.
GenomeReviews	Gene locus BR0074 in contig AE014291_GR.
KEGG	bms:BR0074.
NMPDR	fig|204722.1.peg.73.
PATRIC	17788407. VBIBruSui107850_0077.
TIGR	BR0074.
Phylogenomic databases
HOGENOM	HBG335267.
OMA	RANAIYE.
PhylomeDB	Q8G376.
ProtClustDB	PRK00509.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0074-MONOMER.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR023434. Arginosuc_synth_type_1_subfam. IPR024074. AS_cat/multimer_dom_body. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.90.1260.10. G3DSA:3.90.1260.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01940.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. ArgG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_BRUSU

Accession

Primary (citable) accession number: Q8G376
Secondary accession number(s): G0KAX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program