Reviewed, UniProtKB/Swiss-Prot Q8G374 (RLMN_BRUSU)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase N
    EC=2.1.1.-
Alternative name(s):
    23S rRNA m2A2503 methyltransferase

Gene names

Name:	rlmN
Ordered Locus Names:	BR0077

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates position 2 of adenine 2503 in 23S rRNA By similarity.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the radical SAM superfamily. RlmN family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Ribosomal RNA large subunit methyltransferase N HAMAP MF_01849		PRO_0000350067
Sites
	Metal binding	145	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	149	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	152	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G374-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5FEA8B93A8B7A091	FASTA	411	45,850
	10 20 30 40 50 60 MSISFDLTID DTRDQLARHA RASLEAKPSL IGMSREEMAA ALIAAGVPER QVKMRISQLW 70 80 90 100 110 120 HWLYVRGVSD FADMRNISKD LRAMLAQHFT IARPEVVEEQ ISQDGTRKWL FRFPPRGAGR 130 140 150 160 170 180 PVEIESVYIP EEGRGTLCIS SQVGCTLTCS FCHTGTQKLV RNLTSEEILA QLLTARDRLG 190 200 210 220 230 240 DFPDKDTPDG AMVPAEGRKI TNIVMMGMGE PLYNFEEVKK ALLIASDGDG PSLSKRRITL 250 260 270 280 290 300 STSGVVPEIY RTGDEIGVML AISLHAVRDE LRDILVPINK KYPLAELIKA CREYPGLSNA 310 320 330 340 350 360 KRITFEYVML KDINDSLDDA KLLVKLLQGI PAKINLIPFN PWPGTNYQCS DWEQIEKFAD 370 380 390 400 410 YVNAAGYASP IRTPRGRDIL AACGQLKSES ERLRKSERLA LEAMMIAGHG E

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29033.1.
RefSeq	NP_697118.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165734.
GenomeReviews	Gene locus BR0077 in contig AE014291_GR.
KEGG	bms:BR0077.
NMPDR	fig|204722.1.peg.75.
TIGR	BR0077.
Phylogenomic databases
HOGENOM	HBG335345.
OMA	TFCSTAQ.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0077-MON.
Family and domain databases
HAMAP	MF_01849. [Tree]
InterPro	IPR006638. Elp3/MiaB/NifB. IPR007197. Radical_SAM. IPR004383. rRNA_lsu_MTrfase_RlmN. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF006004. CHP00048. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMN_BRUSU

Accession

Primary (citable) accession number: Q8G374

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2003
Last modified:	December 15, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)