Reviewed, UniProtKB/Swiss-Prot Q8G353 (ILVD_BRUSU)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BR0099

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103445
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G353-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3DC7F16B2CA75093	FASTA	611	65,344
	10 20 30 40 50 60 MPPYRSRTTT HGRNMAGARG LWRATGMKDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIESAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMEA GKVVWEDSVK KLDLVDAMVA AADDHYTDEQ 190 200 210 220 230 240 VKAIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS TLATHADRKR LFVEAGHLIV 250 260 270 280 290 300 DLARRYYEQD DESVLPRSIA TFSAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMAD 310 320 330 340 350 360 IDRLSRRVPV LCKVAPAVSS VHMEDVHHAG GIMGILGQLD NAGLLTTSIP TVHSETLAKA 370 380 390 400 410 420 LDHWDVTRTN SEMVHKFYSA APGGVPTQVA FSQERRFDKV DTDREKGVIR SKEHAFSQDG 430 440 450 460 470 480 GLAVLYGNLA EDGCIVKTAG VDDSILKFSG PARIFESQDS AVLGILNGKI KPGDIVLIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGFGKACAL ITDGRFSGGS SGLSIGHVSP EAAEGGTIGL 550 560 570 580 590 600 VREGDIIDID IPNRKIHLAV DDATLAERRA EQDAAGWKPA EERKRKISTA LKAYAAMATS 610 AARGAVRKLP D

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29055.1.
RefSeq	NP_697140.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165756.
GenomeReviews	Gene locus BR0099 in contig AE014291_GR.
KEGG	bms:BR0099.
NMPDR	fig|204722.1.peg.97.
TIGR	BR0099.
Phylogenomic databases
HOGENOM	Q8G353.
OMA	SRKVPCL
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0099-MON.
BRENDA	4.2.1.9. 281610.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BRUSU

Accession

Primary (citable) accession number: Q8G353

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)