Q8G353 (ILVD_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BR0099, BS1330_I0099

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103445
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G353 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3DC7F16B2CA75093	FASTA	611	65,344
	10 20 30 40 50 60 MPPYRSRTTT HGRNMAGARG LWRATGMKDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIESAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMEA GKVVWEDSVK KLDLVDAMVA AADDHYTDEQ 190 200 210 220 230 240 VKAIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS TLATHADRKR LFVEAGHLIV 250 260 270 280 290 300 DLARRYYEQD DESVLPRSIA TFSAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMAD 310 320 330 340 350 360 IDRLSRRVPV LCKVAPAVSS VHMEDVHHAG GIMGILGQLD NAGLLTTSIP TVHSETLAKA 370 380 390 400 410 420 LDHWDVTRTN SEMVHKFYSA APGGVPTQVA FSQERRFDKV DTDREKGVIR SKEHAFSQDG 430 440 450 460 470 480 GLAVLYGNLA EDGCIVKTAG VDDSILKFSG PARIFESQDS AVLGILNGKI KPGDIVLIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGFGKACAL ITDGRFSGGS SGLSIGHVSP EAAEGGTIGL 550 560 570 580 590 600 VREGDIIDID IPNRKIHLAV DDATLAERRA EQDAAGWKPA EERKRKISTA LKAYAAMATS 610 AARGAVRKLP D

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29055.1. CP002997 Genomic DNA. Translation: AEM17467.1.
RefSeq	NP_697140.1. NC_004310.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165756.
GenomeReviews	Gene locus BR0099 in contig AE014291_GR.
KEGG	bms:BR0099.
NMPDR	fig|204722.1.peg.97.
PATRIC	17788456. VBIBruSui107850_0101.
TIGR	BR0099.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q8G353.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0099-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BRUSU

Accession

Primary (citable) accession number: Q8G353
Secondary accession number(s): G0KAZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program