Q8G2Y6 (GRPE_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein grpE

Alternative name(s):
HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	BR0171, BS1330_I0171

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding By similarity. HAMAP MF_01151
Subunit structure	Homodimer By similarity. HAMAP MF_01151
Subcellular location	Cytoplasm Probable HAMAP MF_01151.
Sequence similarities	Belongs to the grpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro chaperone binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Protein grpE HAMAP MF_01151		PRO_0000113756

Sequences

	Sequence		Length	Mass (Da)
	Q8G2Y6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C6A377466434FC11	FASTA	230	25,310
	10 20 30 40 50 60 MADEKNKPEN PDLDQRDINN PRDREALKQA ADDFLKARRA EARAEAAADE AEGEVDETAN 70 80 90 100 110 120 RIAVLEADNT ELKDQMLRVA AEMENLRKRT QRDVQDARAY AITNFARDML SVSDNLRRAL 130 140 150 160 170 180 DAIPADALEA DSNLKSLSEG VEMTERAMLL ALERHGVKKL EPEGQKFDPN FHQAMFEVPN 190 200 210 220 230 PDLPNNTVVQ VVQAGYAIGD RVLRPAMVGV SKGGPKVSAE NGASTSEDNA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29124.1. CP002997 Genomic DNA. Translation: AEM17536.1.
RefSeq	NP_697209.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G2Y6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165828.
GenomeReviews	Gene locus BR0171 in contig AE014291_GR.
KEGG	bms:BR0171.
NMPDR	fig|204722.1.peg.166.
PATRIC	17788610. VBIBruSui107850_0178.
TIGR	BR0171.
Phylogenomic databases
HOGENOM	HBG732630.
OMA	HEIYEIK.
PhylomeDB	Q8G2Y6.
ProtClustDB	PRK14141.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0171-MONOMER.
Family and domain databases
HAMAP	MF_01151. GrpE. [Tree]
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
Gene3D	G3DSA:3.90.20.20. GrpE_coiled_coil. 1 hit. G3DSA:2.30.22.10. GrpE_head. 1 hit.
KO	K03687.
PANTHER	PTHR21237. GrpE. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
SUPFAM	SSF51064. GrpE_head. 1 hit.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_BRUSU

Accession

Primary (citable) accession number: Q8G2Y6
Secondary accession number(s): G0KBE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program