Reviewed, UniProtKB/Swiss-Prot Q8G2Y6 (GRPE_BRUSU)

Names and origin

Protein names

Recommended name:
    Protein grpE
Alternative name(s):
    HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	BR0171

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the grpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro protein import into mitochondrial matrix Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: InterPro
Molecular function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro chaperone binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Protein grpE HAMAP MF_01151		PRO_0000113756

Sequences

	Sequence		Length	Mass (Da)
	Q8G2Y6-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C6A377466434FC11	FASTA	230	25,310
	10 20 30 40 50 60 MADEKNKPEN PDLDQRDINN PRDREALKQA ADDFLKARRA EARAEAAADE AEGEVDETAN 70 80 90 100 110 120 RIAVLEADNT ELKDQMLRVA AEMENLRKRT QRDVQDARAY AITNFARDML SVSDNLRRAL 130 140 150 160 170 180 DAIPADALEA DSNLKSLSEG VEMTERAMLL ALERHGVKKL EPEGQKFDPN FHQAMFEVPN 190 200 210 220 230 PDLPNNTVVQ VVQAGYAIGD RVLRPAMVGV SKGGPKVSAE NGASTSEDNA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29124.1.
RefSeq	NP_697209.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165828.
GenomeReviews	Gene locus BR0171 in contig AE014291_GR.
KEGG	bms:BR0171.
NMPDR	fig|204722.1.peg.166.
TIGR	BR0171.
Phylogenomic databases
HOGENOM	HBG732630.
OMA	IENVMEN.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0171-MON.
Family and domain databases
HAMAP	MF_01151. [Tree]
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
Gene3D	G3DSA:3.90.20.20. GrpE_coiled_coil. 1 hit. G3DSA:2.30.22.10. GrpE_head. 1 hit.
PANTHER	PTHR21237. GrpE. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_BRUSU

Accession

Primary (citable) accession number: Q8G2Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	March 1, 2003
Last modified:	December 15, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)