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Q8G2Y6 (GRPE_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GrpE
Alternative name(s):
HSP-70 cofactor
Gene names
Name:grpE
Ordered Locus Names:BR0171, BS1330_I0171
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01151

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01151.

Sequence similarities

Belongs to the GrpE family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionadenyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Protein GrpE HAMAP-Rule MF_01151
PRO_0000113756

Sequences

Sequence LengthMass (Da)Tools
Q8G2Y6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: C6A377466434FC11

FASTA23025,310
        10         20         30         40         50         60 
MADEKNKPEN PDLDQRDINN PRDREALKQA ADDFLKARRA EARAEAAADE AEGEVDETAN 

        70         80         90        100        110        120 
RIAVLEADNT ELKDQMLRVA AEMENLRKRT QRDVQDARAY AITNFARDML SVSDNLRRAL 

       130        140        150        160        170        180 
DAIPADALEA DSNLKSLSEG VEMTERAMLL ALERHGVKKL EPEGQKFDPN FHQAMFEVPN 

       190        200        210        220        230 
PDLPNNTVVQ VVQAGYAIGD RVLRPAMVGV SKGGPKVSAE NGASTSEDNA 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN29124.1.
CP002997 Genomic DNA. Translation: AEM17536.1.
RefSeqNP_697209.1. NC_004310.3.
YP_005615028.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8G2Y6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR0171.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN29124; AAN29124; BR0171.
AEM17536; AEM17536; BS1330_I0171.
GeneID1165828.
12138712.
KEGGbms:BR0171.
bsi:BS1330_I0171.
PATRIC17788610. VBIBruSui107850_0178.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0576.
HOGENOMHOG000252083.
KOK03687.
OMAKEPEFCE.
OrthoDBEOG6FJNJ9.

Family and domain databases

Gene3D2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPMF_01151. GrpE.
InterProIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERPTHR21237. PTHR21237. 1 hit.
PfamPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSPR00773. GRPEPROTEIN.
SUPFAMSSF51064. SSF51064. 1 hit.
PROSITEPS01071. GRPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRPE_BRUSU
AccessionPrimary (citable) accession number: Q8G2Y6
Secondary accession number(s): G0KBE4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names