Q8G2W6 (CYSD_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	BR0193, BS1330_I0193

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340187

Sequences

	Sequence		Length	Mass (Da)
	Q8G2W6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A2EC938F2C141D7A	FASTA	300	34,743
	10 20 30 40 50 60 MKNLTHLQRL EAEAIHVFRE VAATFSNPVM LYSVGKDSSV MLHLAMKAFY PAPPPFPFLH 70 80 90 100 110 120 VDTTWKFREM IEFRDAQARE KGFELLVHVN EQGVRDGIGP FTHGSNVHTH IMKTVGLRQA 130 140 150 160 170 180 LDKYRFDAAF GGARRDEEKS RAKERIFSFR NAQHGWDPKN QRPEMWKIYN TRVSKGESIR 190 200 210 220 230 240 VFPLSNWTEL DIWQYILQEN IPIVPLYFAA RRPVVERDGM LIMVDDDRMK LRPGEQVENR 250 260 270 280 290 300 LVRFRTLGCY PLTGAIPSSA ANLSDIVEEM LIARTSERQG RAIDRDEAGS MEKKKREGYF

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29146.1. CP002997 Genomic DNA. Translation: AEM17558.1.
RefSeq	NP_697231.1. NC_004310.3.
3D structure databases
HSSP	HSSP built from PDB template 1ZUN based on UniProtKB Q87WW0.
ProteinModelPortal	Q8G2W6.
SMR	Q8G2W6. Positions 4-210.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165850.
GenomeReviews	Gene locus BR0193 in contig AE014291_GR.
KEGG	bms:BR0193.
NMPDR	fig|204722.1.peg.188.
PATRIC	17788656. VBIBruSui107850_0201.
TIGR	BR0193.
Phylogenomic databases
HOGENOM	HBG758022.
OMA	RVIDHDA.
PhylomeDB	Q8G2W6.
ProtClustDB	PRK05253.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0193-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BRUSU

Accession

Primary (citable) accession number: Q8G2W6
Secondary accession number(s): G0KBG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program