Q8G2W6 (CYSD_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	BR0193, BS1330_I0193

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP-Rule MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP-Rule MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Sulfate adenylyltransferase subunit 2 HAMAP-Rule MF_00064		PRO_0000340187

Sequences

	Sequence		Length	Mass (Da)
	Q8G2W6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A2EC938F2C141D7A	FASTA	300	34,743
	10 20 30 40 50 60 MKNLTHLQRL EAEAIHVFRE VAATFSNPVM LYSVGKDSSV MLHLAMKAFY PAPPPFPFLH 70 80 90 100 110 120 VDTTWKFREM IEFRDAQARE KGFELLVHVN EQGVRDGIGP FTHGSNVHTH IMKTVGLRQA 130 140 150 160 170 180 LDKYRFDAAF GGARRDEEKS RAKERIFSFR NAQHGWDPKN QRPEMWKIYN TRVSKGESIR 190 200 210 220 230 240 VFPLSNWTEL DIWQYILQEN IPIVPLYFAA RRPVVERDGM LIMVDDDRMK LRPGEQVENR 250 260 270 280 290 300 LVRFRTLGCY PLTGAIPSSA ANLSDIVEEM LIARTSERQG RAIDRDEAGS MEKKKREGYF

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29146.1. CP002997 Genomic DNA. Translation: AEM17558.1.
RefSeq	NP_697231.1. NC_004310.3. YP_005615050.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G2W6.
SMR	Q8G2W6. Positions 4-210.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0193.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29146; AAN29146; BR0193. AEM17558; AEM17558; BS1330_I0193.
GeneID	1165850. 12138734.
KEGG	bms:BR0193. bsi:BS1330_I0193.
PATRIC	17788656. VBIBruSui107850_0201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HOG000263604.
KO	K00957.
OMA	MGCSPFV.
ProtClustDB	PRK05253.
Enzyme and pathway databases
UniPathway	UPA00140; UER00204.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00064. Sulf_adenylyltr_sub2.
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BRUSU

Accession

Primary (citable) accession number: Q8G2W6
Secondary accession number(s): G0KBG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program