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Q8G2U9 (THIG_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thiazole synthase

EC=2.8.1.10
Gene names
Name:thiG
Ordered Locus Names:BR0215, BS1330_I0215
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S By similarity. HAMAP-Rule MF_00443

Catalytic activity

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O. HAMAP-Rule MF_00443

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00443

Subunit structure

Homotetramer. Forms heterodimers with either ThiH or ThiS By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00443.

Sequence similarities

Belongs to the ThiG family.

Ontologies

Keywords
   Biological processThiamine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processthiamine diphosphate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function2-iminoacetate synthase activity

Inferred from electronic annotation. Source: InterPro

sulfurtransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256Thiazole synthase HAMAP-Rule MF_00443
PRO_0000162798

Regions

Region184 – 1852DXP binding By similarity
Region206 – 2072DXP binding By similarity

Sites

Active site961Schiff-base intermediate with DXP By similarity
Binding site1571DXP; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G2U9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: B4BB14EEA3EA18D0

FASTA25627,351
        10         20         30         40         50         60 
MLEFYGKRFE SRLLLGTAQY PSPSILADAV RASLSRIVTV SLRRESGEAR AGQDFWALIK 

        70         80         90        100        110        120 
ALGVAVLPNT AGCHTPREAI TTAHMAREVF GTNWIKLEVI GDTDTLQPDP FGLVEAARIL 

       130        140        150        160        170        180 
CDESFEVFPY MNDDLIVAER LIEAGCKVLM PWGAPIGSGR GLNNPYALKT MRAHFPDIPL 

       190        200        210        220        230        240 
VVDAGIGVPS HAAAAMELGF DAVLINTAVA KAGDPAAMAR AFALAVEAGR LAYEADPIEA 

       250 
RDMASPSTPL LGKAFL 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN29164.1.
CP002997 Genomic DNA. Translation: AEM17576.1.
RefSeqNP_697249.1. NC_004310.3.
YP_005615068.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8G2U9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR0215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN29164; AAN29164; BR0215.
AEM17576; AEM17576; BS1330_I0215.
GeneID1165873.
12138757.
KEGGbms:BR0215.
bsi:BS1330_I0215.
PATRIC17788699. VBIBruSui107850_0221.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2022.
HOGENOMHOG000248049.
KOK03149.
OMAGSQLVTM.
OrthoDBEOG6KMBD9.

Enzyme and pathway databases

UniPathwayUPA00060.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00443. ThiG.
InterProIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
PfamPF05690. ThiG. 1 hit.
[Graphical view]
SUPFAMSSF110399. SSF110399. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIG_BRUSU
AccessionPrimary (citable) accession number: Q8G2U9
Secondary accession number(s): G0KBR3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella suis

Brucella suis (strain 1330): entries and gene names