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Q8G2U9

- THIG_BRUSU

UniProt

Q8G2U9 - THIG_BRUSU

Protein

Thiazole synthase

Gene

thiG

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

    Catalytic activityi

    1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei96 – 961Schiff-base intermediate with DXPUniRule annotation
    Binding sitei157 – 1571DXP; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. 2-iminoacetate synthase activity Source: InterPro
    2. sulfurtransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
    Gene namesi
    Name:thiGUniRule annotation
    Ordered Locus Names:BR0215, BS1330_I0215
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256Thiazole synthasePRO_0000162798Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

    Protein-protein interaction databases

    STRINGi204722.BR0215.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8G2U9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni184 – 1852DXP bindingUniRule annotation
    Regioni206 – 2072DXP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ThiG family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2022.
    HOGENOMiHOG000248049.
    KOiK03149.
    OMAiGSQLVTM.
    OrthoDBiEOG6KMBD9.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00443. ThiG.
    InterProiIPR013785. Aldolase_TIM.
    IPR008867. ThiG.
    [Graphical view]
    PfamiPF05690. ThiG. 1 hit.
    [Graphical view]
    SUPFAMiSSF110399. SSF110399. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8G2U9-1 [UniParc]FASTAAdd to Basket

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    MLEFYGKRFE SRLLLGTAQY PSPSILADAV RASLSRIVTV SLRRESGEAR    50
    AGQDFWALIK ALGVAVLPNT AGCHTPREAI TTAHMAREVF GTNWIKLEVI 100
    GDTDTLQPDP FGLVEAARIL CDESFEVFPY MNDDLIVAER LIEAGCKVLM 150
    PWGAPIGSGR GLNNPYALKT MRAHFPDIPL VVDAGIGVPS HAAAAMELGF 200
    DAVLINTAVA KAGDPAAMAR AFALAVEAGR LAYEADPIEA RDMASPSTPL 250
    LGKAFL 256
    Length:256
    Mass (Da):27,351
    Last modified:March 1, 2003 - v1
    Checksum:iB4BB14EEA3EA18D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29164.1.
    CP002997 Genomic DNA. Translation: AEM17576.1.
    RefSeqiNP_697249.1. NC_004310.3.
    YP_005615068.1. NC_017251.1.

    Genome annotation databases

    EnsemblBacteriaiAAN29164; AAN29164; BR0215.
    AEM17576; AEM17576; BS1330_I0215.
    GeneIDi1165873.
    12138757.
    KEGGibms:BR0215.
    bsi:BS1330_I0215.
    PATRICi17788699. VBIBruSui107850_0221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29164.1 .
    CP002997 Genomic DNA. Translation: AEM17576.1 .
    RefSeqi NP_697249.1. NC_004310.3.
    YP_005615068.1. NC_017251.1.

    3D structure databases

    ProteinModelPortali Q8G2U9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 204722.BR0215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN29164 ; AAN29164 ; BR0215 .
    AEM17576 ; AEM17576 ; BS1330_I0215 .
    GeneIDi 1165873.
    12138757.
    KEGGi bms:BR0215.
    bsi:BS1330_I0215.
    PATRICi 17788699. VBIBruSui107850_0221.

    Phylogenomic databases

    eggNOGi COG2022.
    HOGENOMi HOG000248049.
    KOi K03149.
    OMAi GSQLVTM.
    OrthoDBi EOG6KMBD9.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00443. ThiG.
    InterProi IPR013785. Aldolase_TIM.
    IPR008867. ThiG.
    [Graphical view ]
    Pfami PF05690. ThiG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110399. SSF110399. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.

    Entry informationi

    Entry nameiTHIG_BRUSU
    AccessioniPrimary (citable) accession number: Q8G2U9
    Secondary accession number(s): G0KBR3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3