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Q8G2U9

- THIG_BRUSU

UniProt

Q8G2U9 - THIG_BRUSU

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Protein

Thiazole synthase

Gene

thiG

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961Schiff-base intermediate with DXPUniRule annotation
Binding sitei157 – 1571DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. 2-iminoacetate synthase activity Source: InterPro
  2. sulfurtransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:BR0215, BS1330_I0215
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Thiazole synthasePRO_0000162798Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi204722.BR0215.

Structurei

3D structure databases

ProteinModelPortaliQ8G2U9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1852DXP bindingUniRule annotation
Regioni206 – 2072DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2022.
HOGENOMiHOG000248049.
KOiK03149.
OMAiGSQLVTM.
OrthoDBiEOG6KMBD9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
PfamiPF05690. ThiG. 1 hit.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G2U9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLEFYGKRFE SRLLLGTAQY PSPSILADAV RASLSRIVTV SLRRESGEAR
60 70 80 90 100
AGQDFWALIK ALGVAVLPNT AGCHTPREAI TTAHMAREVF GTNWIKLEVI
110 120 130 140 150
GDTDTLQPDP FGLVEAARIL CDESFEVFPY MNDDLIVAER LIEAGCKVLM
160 170 180 190 200
PWGAPIGSGR GLNNPYALKT MRAHFPDIPL VVDAGIGVPS HAAAAMELGF
210 220 230 240 250
DAVLINTAVA KAGDPAAMAR AFALAVEAGR LAYEADPIEA RDMASPSTPL

LGKAFL
Length:256
Mass (Da):27,351
Last modified:March 1, 2003 - v1
Checksum:iB4BB14EEA3EA18D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29164.1.
CP002997 Genomic DNA. Translation: AEM17576.1.
RefSeqiNP_697249.1. NC_004310.3.
YP_005615068.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN29164; AAN29164; BR0215.
AEM17576; AEM17576; BS1330_I0215.
GeneIDi1165873.
12138757.
KEGGibms:BR0215.
bsi:BS1330_I0215.
PATRICi17788699. VBIBruSui107850_0221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29164.1 .
CP002997 Genomic DNA. Translation: AEM17576.1 .
RefSeqi NP_697249.1. NC_004310.3.
YP_005615068.1. NC_017251.1.

3D structure databases

ProteinModelPortali Q8G2U9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 204722.BR0215.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN29164 ; AAN29164 ; BR0215 .
AEM17576 ; AEM17576 ; BS1330_I0215 .
GeneIDi 1165873.
12138757.
KEGGi bms:BR0215.
bsi:BS1330_I0215.
PATRICi 17788699. VBIBruSui107850_0221.

Phylogenomic databases

eggNOGi COG2022.
HOGENOMi HOG000248049.
KOi K03149.
OMAi GSQLVTM.
OrthoDBi EOG6KMBD9.

Enzyme and pathway databases

UniPathwayi UPA00060 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00443. ThiG.
InterProi IPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view ]
Pfami PF05690. ThiG. 1 hit.
[Graphical view ]
SUPFAMi SSF110399. SSF110399. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiTHIG_BRUSU
AccessioniPrimary (citable) accession number: Q8G2U9
Secondary accession number(s): G0KBR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3