Reviewed, UniProtKB/Swiss-Prot Q8G2Q1 (URED1_BRUSU)

Names and origin

Protein names

Recommended name:
    Urease accessory protein ureD 1

Gene names

Name:	ureD1
Synonyms:	ureD-1
Ordered Locus Names:	BR0267

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.2
Subunit structure	UreD, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ureD family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Urease accessory protein ureD 1 HAMAP MF_01384		PRO_0000340427

Sequences

	Sequence		Length	Mass (Da)
	Q8G2Q1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2555CA77107D953D	FASTA	280	30,230
	10 20 30 40 50 60 MLIINDNNLS GLSLQRVNGT GELSVQFKDG RSRISRLYQE GAAKIRMPQA VTGPLEAILI 70 80 90 100 110 120 NTSGGLTGGD RLKWDVALDD GASAVITTQA CERIYRSGGG EARIATRLKA AKGTRLAWLP 130 140 150 160 170 180 QETILFNRSI LSRRLDVELE EGAQMLVVEA TVFGRLAMGE RVVAARFADR WRVRLGGRVI 190 200 210 220 230 240 HAEEFRLGPD VGAELQAPAV AGGACAMATV LMVCEQAGRH LETARAIIGE EGGCSLWRVG 250 260 270 280 KASKLVVRLY APDSYALRRR LCPLVALLNG KAGLPKVWTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.
[2]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed: 17578575] [Abstract] Cited for: OPERON DISRUPTION, ROLE IN VIRULENCE. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29216.1.
RefSeq	NP_697301.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165927.
GenomeReviews	Gene locus BR0267 in contig AE014291_GR.
KEGG	bms:BR0267.
NMPDR	fig|204722.1.peg.258.
TIGR	BR0267.
Phylogenomic databases
HOGENOM	Q8G2Q1.
OMA	QETILFD
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0267-MON.
Family and domain databases
HAMAP	MF_01384. [Tree]
InterPro	IPR002669. UreD. [Graphical view]
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URED1_BRUSU

Accession

Primary (citable) accession number: Q8G2Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)